Skip to main content

Influence of mutation rate on estimators of genetic differentiation - lessons from Arabidopsis thaliana

An Erratum to this article was published on 07 October 2010

Abstract

Background

The analysis of molecular variation within and between populations is crucial to establish strategies for conservation as well as to detect the footprint of spatially heterogeneous selection. The traditional estimator of genetic differentiation (F ST ) has been shown to be misleading if genetic diversity is high. Alternative estimators of F ST have been proposed, but their robustness to variation in mutation rate is not clearly established. We first investigated the effect of mutation and migration rate using computer simulations and examined their joint influence on Q ST , a measure of genetic differentiation for quantitative traits. We further used experimental data in natural populations of Arabidopsis thaliana to characterize the effect of mutation rate on various estimates of population differentiation. Since natural species exhibit various degrees of self-fertilisation, we also investigated the effect of mating system on the different estimators.

Results

If mutation rate is high and migration rate low, classical measures of genetic differentiation are misleading. Only Φ ST , an estimator that takes the mutational distances between alleles into account, is independent of mutation rate, for all migration rates. However, the performance of Φ ST depends on the underlying mutation model and departures from this model cause its performance to degrade. We further show that Q ST has the same bias. We provide evidence that, in A. thaliana, microsatellite variation correlates with mutation rate. We thereby demonstrate that our results on estimators of genetic differentiation have important implications, even for species that are well established models in population genetics and molecular biology.

Conclusions

We find that alternative measures of differentiation like F' ST and D are not suitable for estimating effective migration rate and should not be used in studies of local adaptation. Genetic differentiation should instead be measured using an estimator that takes mutation rate into account, such as Φ ST . Furthermore, in systems where migration between populations is low, such as A. thaliana, Q ST < F ST cannot be taken as evidence for homogenising selection as has been traditionally thought.

Background

Characterisation of population structure is a pivotal task in population genetics. It is important for inferring the evolutionary history of a species, assisting in conservation studies [1] and measuring dispersal [2–4]. In association mapping and forensic applications, population structure must be corrected for [see [5]]. The characterisation of population structure is also crucial for studies tracking the signature of local adaptation i. e. when natural selection maintains differences in allele frequencies between populations. Indeed, limited migration or population bottlenecks can also cause allele frequencies to diverge. A proper characterisation of population structure is absolutely required to infer the action of local selection on outlier loci with reasonable confidence [6].

One way to quantify population structure is to use the summary statistic F ST , which measures population differentiation. If allele frequencies are similar in a set of populations F ST will be low, and conversely, if allele frequencies are different F ST will be high. Basically, F ST and its hierarchical extensions quantify how genetic diversity is partitioned within and between populations or groups of populations (see [5, 7–9] for a review of the large body of theory on F ST ). F ST can also be defined in terms of the ratio of coalescence times, for pairs of alleles from the same population over pairs of alleles from different populations [7]. If we consider only identity by descent, the topology of the genealogy is independent of the number of mutations.

F ST can be sometimes related to quantities describing migration [2], although this approach has its caveats [4]. In the context of selection, by using many presumably neutral markers one can build a distribution of expected F ST values and then compare these to F ST values of genes that are hypothesised to be subject to selection [9]. This can also be done for phenotypes using Q ST , a measure of genetic differentiation in quantitative traits. It has been shown that under neutrality Q ST is equivalent to a single locus F ST , and this relationship is robust to different demographic scenarios [10–12]. This property can be used to compare the Q ST of a quantitative trait to the distribution of F ST from neutral markers and infer whether the trait is influenced by selection [reviewed in [13]].

Some recent studies have raised concerns about the reliability of F ST for characterisation of population structure using markers with high mutation rates, such as microsatellites [14–18]. High levels of within population diversity bias F ST estimates downwards, because F ST is estimated using heterozygosities or genetic variances. If a locus has multiple alleles, classical F ST can be low even if populations share no alleles [15, 16, 19]. In addition to the classical F ST , there are other estimators that have been proposed over the years. An analogous estimator to F ST , Φ ST , takes into account the distances between alleles thereby correcting for mutation rate [7, 20]. Classical F ST , estimated in the framework of Weir & Cockerham [21] considers only allele identity while Φ ST considers distances between the alleles, be it differences in repeat number (e.g. in the case of microsatellite) or number of pairwise differences between DNA haplotypes. Another measure, F' ST , standardises the observed F ST value with the maximum possible value that F ST could attain given the amount of observed diversity [15]. Finally, Jost derived recently a new measure of genetic differentiation, D[16] to replace G ST (or its equivalent for empirical studies F ST ). D measures allelic differentiation by partitioning heterozygosity into within and between population components [16]. D is defined as , where H T is total heterozygosity, H S subpopulation heterozygosity and n is the number of subpopulations [16]. These estimators provide improved measures of allelic differentiation between populations, yet their usefulness for estimating genetic differentiation in the context of migration or local adaptation is not clearly established. Most previous studies addressed these concerns using G ST instead of F ST . When G ST is used, the mathematics are less complicated. In practise, however, F ST has better statistical properties when empirical studies are concerned [see [5]]. For this reason, F ST is the statistic of interest in this study. Moreover, in practise the difference between these two statistics is small.

Here, we characterize the relationship between different estimators of genetic differentiation and mutation rate using simulations as well as experimental data collected from natural populations. Our study system is Arabidopsis thaliana (L.) Heyhn. (Brassicaceae). We use simulations to characterize the relationship between mutation rate and population differentiation for both molecular markers and quantitative traits and ask the following questions: i) are all estimators of genetic differentiation dependent on mutation rate, ii) is the estimator of differentiation for quantitative traits dependent on mutation rate, iii) does the mating system influence the relationship between mutation rate and estimators of population differentiation. We provide empirical data from Arabidopsis thaliana, a long-standing model for plant molecular biology that has recently become a model in plant population genetics [22]. We show that in A. thaliana, F ST is biased downwards for high mutation rate loci. We discuss these results and their implications for various applications of F ST analysis, including the reliable detection of variable selection.

Methods

Data analysis - Computer simulations

In order to investigate the behaviour of F ST , F' ST , Φ ST and D under high mutation rates, computer simulations using EasyPop 1.8 [23] were performed. The simulation scheme was set to 10 populations with 500 individuals each, 20 freely recombining loci and random mating hermaphrodites. All loci were set initially as polymorphic by allowing EasyPop to generate the genotypes in the first generation. Populations followed an island model of migration. Migration rates (probability that a given individual will migrate in each generation), m ranged from 0.1 to 0.00001 and mutation rates (probability that a given allele will mutate in each generation), μ from 0.00001 to 0.01. In order to simulate microsatellite loci we first examined a pure single step mutation model. Then we relaxed this assumption by using a mixed mutation model in which the loci followed a single step mutation model but with the probability of 0.2 to mutate to any state. The number of possible allelic states was set to 30. The effect of self-fertilisation was examined by doing simulations with proportion of self-fertilisation set to 0.9. Simulations were run for 2000 generations. In our simulations this was enough for F ST to reach equilibrium. To simulate a realistic sampling situation, 30 individuals were finally sampled from each population for parameter estimation. Each simulation was repeated 5 times for a given set of parameter values. For each simulated dataset we calculated the estimators of genetic differentiation as described below.

Next we examined how mutation rate at underlying QTL affects Q ST . We used quantiNEMO [24], with the same settings as described above for neutral markers with the following exceptions: the number of QTL underlying the variation in the quantitative trait was 10 and there were 21 possible allelic states for each QTL. We used the random mutation model in quantiNEMO for the QTL alleles, in this model, allelic effects are drawn from a normal distribution, all effects are additive in our simulations. We also ran the simulations using the incremental mutation model, where the allelic effect of a new mutation resembles its ancestor. Variance of allelic effects was set to 0.1. The simulated quantitative trait was neutral and did not have any effect on fitness. The simulation was started at a state where all loci were monomorphic, the number of generations was 4000. The time to reach equilibrium was longer for low migration and mutation rates and in these cases number of generations was 6000. This is longer than for neutral markers because simulations had to be started from a monomorphic state; otherwise distribution of allelic effects becomes unrealistic. Variance components for Q ST were estimated from genotypic values, which are returned by quantiNEMO as output, using R-scripts written by IK. The statistical model was a mixed-effect model with populations as random factors; REML-estimates of variance components were used. This was done in order to calculate Q ST also in the presence of self-fertilisation, which quantiNEMO does not calculate as a standard output. Q ST was estimated from the equation [25], where is the between populations genetic variance component, is the within populations genetic variance component and F IS is reduction of heterozygosity within individuals due to inbreeding. Our method of estimating Q ST gives the same results as the standard output of quantiNEMO when mating is random (results not shown). For each parameter set, 50 replicates of simulations with a single quantitative trait were run.

We also performed coalescent simulations to investigate the effect of different marker types on F ST calculations. We investigated DNA haplotypes (these would be derived by re-sequencing short fragments, one locus is one fragment), independent single SNP markers and microsatellite markers following a single step mutation model. All coalescent simulations were performed using the program ms [26]. We simulated an island model of population structure with 10 populations, 20 individuals were sampled from each population. For sequence haplotypes and microsatellites 30 independent loci were simulated, for SNP markers we simulated 100 independent SNPs. For single SNPs and haplotypes, multiple hits were not permitted. The microsatellite mutation model was implemented via R-script. In the program ms migration and mutation rate are expressed in terms of effective population size, 4Nm and 4Nμ respectively. We set up the simulations so that the effective population size was 1000 for each population and then parameters m and μ ranged from 0.0001 to 0.1 for m and 0.00001 to 0.001 for μ. Each simulation was repeated 5 times for each parameter combination.

Data analysis - Genetic diversity and population structure

All statistical analyses were done using the statistical environment R [27] unless otherwise stated. Methods not implemented by R-packages were implemented via R-scripts written by IK and are available upon request.

Measure of genetic diversity, Nei's gene diversity (Hs) was calculated using FSTAT 2.9.3 [28]. The microsatellite population mutation rate, θ, is the product of effective population size and mutation rate at a locus was calculated following equation 15 of Kimmel et al. [29]. The performance of this summary statistic based method has been shown to be comparable to likelihood-based methods [30]. θ was calculated for each locus within each region. For SNP data the minor allele frequency was calculated for each locus in each region.

F ST was estimated according to Weir & Cockerham [21] for microsatellites and SNP markers, using the R-package "hierfstat" [31]. All other genetic differentiation methods were implemented via R-scripts written by IK. For microsatellites the standardised genetic differentiation measure, F' ST [15], was estimated using the maximised variance component method of Meirmans [32]. In order to take the distance between the microsatellite alleles or sequence haplotypes into account [20] we estimated Φ ST using the method of Michalakis and Excoffier [33]. Differentiation indices between regions were calculated in a hierarchical setting, taking into account the partition of variation between populations within regions [7]. Confidence intervals for different measures of genetic differentiation were generated by bootstrapping over loci. An estimator for D was calculated following Jost [16].

The expected F ST was calculated for the forward and coalescent simulations using the relationship between F ST and coalescence times in the island model. The expected F ST was calculated as [7], where = average coalescence time of alleles from different populations and = average coalescence time of two alleles from the same population. For an island model of population structure, and , where N = population size, d = number of populations and m = migration rate [34]. This provides us with an analytical estimate of the expected value of F ST . We used this as a baseline when comparing the different estimators.

To check whether Φ ST > F ST for the microsatellite loci, we used a permutation test [35]; if Φ ST > F ST stepwise mutations may contribute to genetic differentiation, thereby providing one explanation for the difference. The test was implemented in the program SPAGeDi 1.2 [36]. This is done by permuting microsatellite allele sizes among allelic states to test if stepwise mutations contribute to genetic differentiation.

Population samples

In total 289 individuals from 41 populations were genotyped. Detailed information about the populations can be found in the supplementary material (Additional file 1). We analysed 7, 15, 13 and 6 populations from Spain, France, Norway and Central Asia, respectively. Number of sampled individuals from each population ranges from 3 to 11 with a mean of 7. Three regions in Western Europe: Spain, France, Norway create a South - North cline. The Central Asian region is composed of populations from Kyrgyzstan and Tajikistan. The Spanish populations are described in Pico et al.[37]. French populations were collected by Valerie Le Corre and some of them are described in Le Corre [38]. The Norwegian populations were kindly provided by Odd-Arne Rognli through NARC (Norway). Populations from Central Asia were collected by OL and are described at http://www.inra.fr/vast/collections.htm. Field collected plants were subjected to one or two generations of self-fertilisation in the greenhouse before DNA extraction.

Genotyping

DNA was extracted from young leaves using BioSprint 96 robot and BioSprint 96 DNA Plant Kit (Qiagen) according to manufacturer's instructions.

Plants were genotyped at 20 microsatellite loci. Details of the microsatellite loci used and genotyping procedures can be found in the supplementary material (Additional file 2). Microsatellites were amplified using standard PCR methods and allele sizes were determined using capillary electrophoresis. To determine the actual number of repeats in each allele, the accession Col-0 was genotyped for each locus as a reference. Using the genome sequence and the Col-0 PCR product size the actual number of repeats was deduced for each allele. The Spanish accessions had already been genotyped previously for some of the loci used here, as described in Pico et al.[37]. We verified that our allele sizes corresponded to the allele sizes reported previously by re-genotyping a subsample at selected alleles.

The plants were also genotyped for a set of 149 single nucleotide polymorphism (SNP) markers [developed by [39]] by Sequenom, inc. (San Diego, CA). Detailed description of the SNP markers is found in supplementary material (Additional file 3). Out of the 149 SNP markers, 12 loci had to be excluded because the genotyping failed or because there was no variation, leaving 137 loci used in the analysis. Microsatellite and SNP genotypes can be found in supplementary material (Additional file 4)

Data analysis - SNP ascertainment bias

The SNP markers we used were discovered in the study of Nordborg et al. [40] and subsequently developed for mapping purposes [39]. The criterion for ascertainment was that one allele of a SNP had to be carried by the standard laboratory accession Col-0 and the other allele had to be in high frequency in the rest of the sample. Hereafter we refer to the publicly available dataset of Nordborg et al. as Nordborg data. To investigate the ascertainment bias in our sample we calculated minor allele frequency distribution for all of the SNPs in the Nordborg data and the 137 SNPs used in this study. Because the sample in which the SNPs were discovered is different from the sample in this study ascertainment bias cannot be corrected in a straightforward manner. Therefore, we examined the effect of ascertainment bias on F ST in the dataset from which they were selected. We used the 137 SNPs used in our study to calculate F ST between genetic clusters defined by Nordborg et al.[40] in the Nordborg dataset. Then we sampled 137 SNPs at random from the Nordborg dataset 1000 times and calculated F ST between the genetic clusters for each sampled dataset.

Results

Computer simulations

We used forward population genetic simulations to investigate the behaviour of different estimators with varying migration and mutation rates. The best estimator, in the context of local adaptation, should be robust to mutation rate to allow comparisons between different marker types.

For a low mutation rate, F ST and Φ ST were reasonably good, but F' ST and D differed from the expected value (Table 1), although for extremely small migration rates the situation was reversed. Results of forward population genetic simulations showed that F ST tended to zero when mutation rate increased (Figure 1). Replicate simulations clustered very well showing that there was little variance among replicates. These results follow the analytical expectation presented in Hedrick [15] and the results of Balloux & Goudet [18]. If mutations followed a pure single step model, Φ ST was essentially independent from mutation rate (Figure 1) as expected; this is true for all migration rates. F' ST and D are not independent from mutation rate. In our simulations, we observed that when migration rate was very low, increasing mutation rate up to 0.01 also caused F' ST and D to go downward (Figure 1, panels C and D). If the assumptions of single step mutation model were relaxed, Φ ST had the same trend as F ST although the effect was somewhat weaker (Figure 2). A self-fertilisation rate of 0.9 did not qualitatively alter the results (Additional file 5). We note that, when mutation rate was increased up to 0.01, all estimators decreased (Figure 1). This is not a cause for concern, since this rate is biologically unrealistic. This effect is explained by the limited number of alleles in the simulations, which at high mutation rate can cause homoplasy.

Table 1 Comparison of expected and observed values for F S T
Figure 1
figure 1

Results of computer simulations for single step mutation model. Different estimators of genetic differentiation were plotted against mutation rate. Different lines represent different migration rates. Migration rates 0.1, 0.01, 0.001, 0.0001 and 0.00001 correspond to different lines as indicated by the legend in panel A. Different estimators are F ST , Φ ST , F' ST and D in panels A, B, C and D respectively.

Figure 2
figure 2

Results of computer simulations for mixed mutation model. The effect of mutation rate on genetic differentiation calculated from Φ ST using mixed mutation model. In this model, there was a probability of 0.2 that when a mutation occurs the allele will mutate to any state. Different lines represent different migration rates. Migration rates 0.1, 0.01, 0.001, 0.0001 and 0.00001 correspond to different lines as indicated by the legend.

We then examined the effect of mutation rate at QTL on Q ST . We observed the same behaviour as for F ST . When migration rates are low, increasing the mutation rate at underlying QTLs caused Q ST to take lower values (Figure 3). We also performed the simulations using an increment mutation model for the QTL, where each new mutation had an allelic value that is close to the allelic value of its ancestor. In this case Q ST behaved like Φ ST and was not affected by mutation rate (Figure 3, panel B). Here again, a self-fertilisation rate of 0.9 did not qualitatively alter the results (Additional file 6).

Figure 3
figure 3

Results for computer simulations for Q ST . The effect of mutation rate and migration rate on Q ST . Different lines represent different migration rates. Migration rates 0.1, 0.01, 0.001, 0.0001 and 0.00001 correspond to different lines as indicated by the legend in panel A. Points are means of 50 replicate simulations and vertical lines correspond to 95% confidence intervals. In panel A) results for the random mutation model, panel B) results for the incremental mutation model.

Next we examined the effect of mutation rate on different marker types. We simulated DNA haplotypes (as would be derived by re-sequencing short fragments from multiple individuals), microsatellite markers and single SNP markers. Results from the simulations are presented in figure 4. We calculated Φ ST that takes into account distance between different haplotypes or microsatellite alleles. Applying this method to both haplotypes and microsatellites gave essentially the same results (Figure 4 and Table 1) and Φ ST was independent from mutation rate for both marker types. Single SNP markers also gave F ST values that were nearly identical to the ones obtained with other types of markers (Table 1). This is in accordance with DNA haplotype and microsatellite markers (Figure 4). Therefore, Φ ST for DNA haplotypes, microsatellites (following single step mutation model) and F ST for single SNPs (free of ascertainment bias) gave comparable estimates of differentiation.

Figure 4
figure 4

Results of coalescent simulations for different marker types. The effect of mutation rate on genetic differentiation, calculated from Φ ST . Black lines represent estimates from DNA haplotypes, grey lines are estimates from microsatellite alleles. Different line types represent different migration rates. Migration rates 0.1, 0.01, 0.001 and 0.0001 correspond to different lines as indicated by the legend.

Genetic differentiation in Arabidopsis thaliana

We characterised diversity within 41 populations distributed in four broad geographic regions. We observed that genetic differentiation (F ST ) for microsatellite loci correlates with gene diversity (Hs) (Figure 5). For instance, in the Spanish populations the correlation between Hs and F ST was r = -0.862 (95% CI = -0.944 - -0.678) with p < 0.001 (Table 2). We further examined the correlation between diversity and various alternative estimators of differentiation. There was positive albeit non-linear relationship between Hs and F' ST , (r = 0.479, [95% CI = 0.076 - 0.760], p = 0.033). Φ ST was not correlated with Hs, (r = -0.294, [95% CI = -0.652 - 0.170], p = 0.208). A similar pattern was observed when the population mutation rate (θ) was used instead of Hs. For θ and F ST r = -0.682, p < 0.001, for θ and F' ST r = 0.500, p = 0.025 and for θ and Φ ST r = -0.301, p = 0.197, respectively. Φ ST is independent from genetic diversity and mutation rate in our data, except in Central Asian populations (Table 2, Figure 5).

Table 2 Correlations between genetic diversity and genetic differentiation
Figure 5
figure 5

Correlations between genetic diversity and genetic differentiation in Spanish populations. Gene diversity, Hs, was calculated for each locus and was plotted against different estimators of genetic differentiation. A) F ST B) F' ST C) Φ ST D) D

The 137 polymorphic SNP markers used in this study were biased towards high frequency as shown in Additional file 7, where minor allele frequency is plotted for each SNP in different regions. This was apparent also from comparing the minor allele frequency spectrum of the full Nordborg data and the 137 SNP set (Additional file 7). Using the 137 SNP set we obtained F ST = -0.0018 between clusters defined previously in the Nordborg data [40]. Sampling 137 SNPs at random from the Nordborg data gave us a 95% confidence interval of F ST -0.0051 - 0.0271. This suggests that ascertainment bias perhaps has only a minor effect in our dataset.

Next we calculated measures of genetic differentiation for microsatellites and SNP markers between populations within regions and between regions (Figure 6). For microsatellites, genetic differentiation between populations was the lowest in Spain (F ST = 0.2900, Φ ST = 0.3556), intermediate for France (F ST = 0.4937, Φ ST = 0.6818) and for Asia (F ST = 0.6026, Φ ST = 0.3101) and the highest in Norway (F ST = 0.8004, Φ ST = 0.8128). A similar trend was observed for both microsatellites and SNP markers (Figure 6). However, it should be noted that the confidence intervals were sometimes broad (Figure 6), especially in Central Asia. Genetic differentiation between geographic regions was smaller than between populations within regions (Figure 6). Differentiation measured by F' ST from microsatellites was F' ST = 0.7208 for Spain, 0.8115 for France, 0.9436 for Norway and 0.8413 for Asia. Values for D were 0.6393, 0.6509, 0.7241 and 0.6334 for the Spanish, French, Norwegian and Cental Asian populations respectively.

Figure 6
figure 6

Genetic differentiation within and between regions. Genetic differentiation between populations within regions and between regions. Differentiation was estimated from microsatellite markers using four different estimators (for explanation, see methods). Points are estimates of differentiation over all loci, bars represent its 95% CI, obtained by bootstrapping over loci. For SNP markers F ST was used. Between group values were not calculated for D, because as of yet D has not been hierarchically extended.

We tested whether Φ ST was higher than F ST by using a permutation test that permutes allele sizes between different alleles [35]. If Φ ST is higher than F ST , one possibility is that stepwise mutations contribute to differentiation [35]. Within Spanish populations, the difference is suggestive albeit not significant, 2-sided test p = 0.0629. Within French populations, the difference was significant p = 0.0210. In Norwegian and Asian populations, differences were not significant (p = 0.1009 and p = 0.8561 respectively). This suggests that stepwise mutations may contribute to genetic differentiation in Spain and France. In the Norwegian and Asian populations instead, microsatellite loci possibly exhibit some departure from a stepwise mutation model. However, this does not prove that the loci follow SSM model.

Discussion

Implications for detecting local adaptation

We used computer simulations to analyze the behaviour of different estimators of genetic differentiation as a function of various migration and mutation rates. Our simulations show that D or F' ST both depend on mutation rate (Figure 1). For D, this is lucidly shown by Jost [16] but the fact that F' ST is dependent on mutation rate is not made clear by Hedrick [15]. New mutations increase differentiation between populations, especially if migration rate is low. D and F' ST measure actual allelic differentiation, regardless of the process that generates these differences [41]. This means that F' ST or D are useful for studies where the amount of genetic differentiation is of interest per se, such as in conservation studies [15, 16]. Instead, these estimators become problematic for studies interested in comparing levels of differentiation across loci, as in studies of local adaptation. Also, they cannot be directly related to coalescence times (Table 1). This is not necessarily a criticism of F' ST and D, since D was derived to measure true allelic differentiation. These measures address the question of the partition of genetic diversity among populations, irrespective of the mechanisms by which this pattern arises [16]. Further work is needed to characterise the properties of these estimators.

Our results show that, because it takes distances between different alleles into account, Φ ST is the only estimator that is completely independent from mutation rate (Figure 1) [18, 20]. We also showed that if the assumptions of Φ ST are met, both DNA haplotypes and microsatellites give estimates comparable to F ST calculated for bi-allelic SNPs (Figure 4). In a large empirical dataset in humans [42], where microsatellites analysed using a stepwise mutation model and SNPs gave comparable results, a similar conclusion was reached. In A. thaliana as in many other species, microsatellite loci were often shown to deviate from pure single step mutation model [43–46]. Our simulations further show that this estimator became dependent on mutation rate, if there was some deviation from the single step mutation model (Figure 2). This is also seen in our dataset in some geographic regions, as we find that Φ ST is not different from F ST . Yet, Φ ST might still be preferred over F ST . Even if there are deviations from the SMM model, Φ ST still performs better than F ST (Figure 1, Figure 2).

SNP markers are unlikely to suffer from these issues in F ST estimation because mutation rate for SNP markers is considerably lower than for microsatellites. However, they may have some ascertainment bias. This effect, however, was minor in the 96 genotypes in which our SNP-marker set was ascertained. Although it is not certain that this behaviour would be the same in our set of 41 populations, estimates of differentiation based on SNPs are roughly concordant with Φ ST estimates based on microsatellites (Figure 6). In Norway and central Asia however, microsatellite Φ ST seems to be lower than F ST for SNP markers (Figure 6). In these regions, we do not have any evidence that microsatellites follow a stepwise mutation model, so this seems a likely cause for the discrepancy. However, ascertainment bias cannot be completely excluded. In many cases, confidence intervals for Φ ST are broad in our data, a likely consequence of the high sampling variance displayed by Φ ST type estimators [47]. This consideration suggests that, whenever possible, DNA haplotypes generated by re-sequencing should be used in studies of local adaptation.

It is known that there is considerable variation in mutation rates between different genes due to evolutionary constraints [48]. In order to directly compare differentiation across genes and correctly assign loci subject to local adaptation, mutation rate has to be taken into account. This problem has been considered earlier in the context of detecting loci that have outlier F ST values [49]. The method of Beaumont & Nichols jointly considers heterozygosity and F ST . This method was shown to be robust to mutation rate variation among loci [49]. Yet if the types of markers combined in one analysis are different and mutation rate has a strong influence for one type of marker because of low migration rates, results have to be interpreted with caution. Indeed, this method gave non sensical results with our A. thaliana dataset combining microsatellite and SNP markers (data not shown).

Our results have further implications for studies based on Q ST vs. F ST comparisons, aiming at detecting local adaptation in quantitative traits. A recent meta-analysis of F ST vs. Q ST studies [50] noted that using F' ST would generally change the conclusions of F ST vs. Q ST studies. However, our study shows that using F' ST or D in Q ST studies is not appropriate, because these measures of genetic differentiation are not independent from the high mutation rate of microsatellites. Yet, this is a concern only for those cases where migration rate is low.

Another concern is the fact that Q ST , the estimate for quantitative traits, is affected by the mutation rate at underlying QTLs (Figure 3). In retrospect, Q ST being an equivalent of F ST in the absence of selection [10], this result is not completely surprising. More interesting however, is the parameter range at which this bias is observed. In our simulations we varied the parameter μ, the genic mutation rate. It should be noted that the overall polygenic mutation rate, or variance contributed by mutation in each generation (assuming additive effects), of a quantitative trait is V m = 2 μN L V α [51], where N L is the number of QTL, 2 accounts for diploidy and V α is the variance of allelic effects. In our simulations N L = 10 and V α = 0.1 were held constant in all simulations. This translates into V m of twice as large as genic mutation rate, so that for μ = 0.0001 V m = 0.0002. In our simulations environmental variance, V E , was set to 1 so mutational variance scaled by environmental variance, h2 m = V m / V E , [51, 52] was equal to V m . In our simulations when genic mutation rate of QTL was μ = 0.001, effects on Q ST were rather large when migration rates were low (Figure 3). This corresponded to h2 m = 0.002. It is interesting to note that empirical estimates of mutational heritabilities frequently fall around this value [52]. For instance, h2 m for the well studied trait of bristle number in Drosophila seems to be around 0.0035 or 0.0043. Schultz et al. [53] estimated h2 m for few life-history traits in A. thaliana and found that they fall around 0.003. Estimating mutational heritability empirically is not easy, but empirical data from several sources suggests that for many traits it is around 0.002 - 0.003 [52].

Interestingly, if new alleles display incremental changes of function, Q ST becomes independent of the mutation rate. However, alleles of large effect contributing to quantitative trait variation have been observed frequently in natural populations [reviewed in [54]]. A prominent example is provided by the FRIGIDA locus, a gene contributing to quantitative variation in flowering time in A. thaliana. Several loss-of-function mutations were reported to segregate in natural populations [38]. Thus QTL are likely to frequently deviate from a purely incremental mutation model.

A comforting fact is that high mutation rates will bias Q ST downwards, so if the goal is to study local adaptation, that is, the case where Q ST > F ST is of interest, then the test will remain conservative. Traditionally it was thought that Q ST < F ST would indicate the action of homogenising selection [13], that is, selection for the same phenotypic optima in different populations. Recently this view has been challenged. Firstly, non-additive gene action seems to bias Q ST downwards [11, 55, 56]. Secondly, the large variance in estimating Q ST and in evolutionary outcomes of single locus (trait) may be a even more serious problem [56–58]. Intuitively, systems where F ST is large, because migration is low, offer potentially greatest statistical power to detect Q ST < F ST . Our results instead suggest that the bias in Q ST may be the greatest in such cases and that the utility of Q ST - F ST comparisons may be limited to detecting diversifying selection.

Correlating estimates of population differentiation to levels of diversity has highlighted the effect of mutation rate on estimates of differentiation in Arabidopsis thaliana. To date, several studies have investigated patterns of population structure of A. thaliana, [37, 59–63] but none has examined the effect of mutation rates on estimates of differentiation. We observe this effect in the four distinct geographical regions. Therefore, mutation rates seem to significantly impact estimates of population differentiation in this species. This relationship has also been found in Arabidopsis lyrata, a relative of A. thaliana exhibiting a markedly different life-history and more genetic diversity than A. thaliana[64, 65]. Some fish species also exhibit this relationship. For example, in two subspecies of the triplefin fish, F ST was low and negatively correlated with expected heterozygosity (r = -0.9) [66]. Similarly O'Reilly et al.[67] found a relationship between heterozygosity and F ST in the fish walleye pollock, which they attributed to homoplasy. As shown by our simulations, this problem is more severe in systems where diversity is very high and migration between populations is low (Figure 3). These examples show that a wide variety of organisms are in the parameter space where variation in F ST reflects variation in both migration and mutation rates. Therefore, studies of population differentiation should systematically investigate this effect.

Effect of the mating system

Simulations were performed under random-mating as well as with a self-fertilizing rate of 0.9 and yielded essentially the same results. From a population genetics perspective, self-fertilisation should reduce effective population size and thus coalescence times of alleles within populations [68]. This is precisely what we observe in our simulations: absolute F ST values are higher, but the relationship of the statistics to mutation rate remains qualitatively the same (Additional file 5). This is also true for Q ST (Additional file 6). Thus the effect of self-fertilisation is mainly to increase F ST values, but it does not alter the effect of mutation rates on its estimates. Although a rate of 0.9 is slightly lower than selfing rates reported for A. thaliana so far, we believe that our simulations reflect faithfully the situation in this species [59, 69, 70]. Indeed, there is one study that found the rate to be closer to 0.9 in one particular population [61] and recently Pico et al. [37] found rates to be variable, from 0.97 down to 0.93 in Iberian populations. Considering that some of these studies used a very limited set of markers, it is conceivable that true rates of self-fertilisation are closer to 0.9 than 0.99. This is also evident when more markers and more individuals from a single population are sampled, because much more heterozygosity is uncovered (IK, unpublished data).

Conclusions

In this study, we lift the ambiguity lying over the effect of variable mutation rate on estimates of population differentiation and show that virtually all estimates show a dependence on higher mutation rates if migration is low. Only Φ ST , which takes mutation rate into account, controls this bias. However, in practise, Φ ST assumes a stepwise mutation model for microsatellites, which may not be correct in all cases. Several microsatellite mutation models have been proposed [46]. Future studies could examine the possibility of incorporating different models of mutation in Φ ST estimation. For this, the rules by which distance matrices between alleles or haplotypes are calculated could be modified. For sequence data, changes would be straightforward to incorporate different models of sequence evolution. More importantly, we show that Q ST estimates are not independent of the mutation rate of QTLs underlying quantitative traits for low migration rates. This result is obtained with a model simulating traits with realistic mutational heritabilities. Therefore Q ST < F ST cannot be interpreted as strong evidence for homogenising selection in systems where genetic differentiation is high. Our study in A. thaliana shows that caution is needed when tracking the signature of local adaptation on loci controlling adaptive phenotypes in this and other model species for genetics. Further studies will eventually be needed to address the properties of these various estimates in non-equilibrium populations or to investigate the impact of the genetic architecture of a trait on Q ST .

References

  1. Avise JC: Molecular markers, natural history and evolution. 2004, Sunderland: Sinauer Associates Inc, 2

    Google Scholar 

  2. Rousset F: Genetic Differentiation and Estimation of Gene Flow from F-Statistics Under Isolation by Distance. Genetics. 1997, 145 (4): 1219-1228.

    PubMed Central  CAS  PubMed  Google Scholar 

  3. Ouborg NJ, Piquot Y, van Groenendael JM: Population genetics, molecular markers and the study of dispersal in plants. J Ecol. 1999, 87: 551-568. 10.1046/j.1365-2745.1999.00389.x.

    Article  Google Scholar 

  4. Whitlock MC, McCauley DE: Indirect measures of gene flow and migration: F ST ≠ 1/(4Nm + 1). Heredity. 1999, 82 (Pt 2): 117-125. 10.1038/sj.hdy.6884960.

    Article  PubMed  Google Scholar 

  5. Holsinger KE, Weir BS: Genetics in geographically structured populations: defining, estimating and interpreting F ST . Nat Rev Genet. 2009, 10: 639-650. 10.1038/nrg2611.

    Article  CAS  PubMed  Google Scholar 

  6. Storz JF: Using genome scans of DNA polymorphism to infer adaptive population divergence. Mol Ecol. 2005, 14 (3): 671-688. 10.1111/j.1365-294X.2005.02437.x.

    Article  CAS  PubMed  Google Scholar 

  7. Excoffier L: Analysis of population subdivision. Handbook of statistical genetics. Edited by: Balding DJ, Bishop M, Cannings C. 2007, Chichester, Wiley, 271-307.

    Google Scholar 

  8. Rousset F: Inferences from spatial population genetics. Handbook of statistical genetics. Edited by: Balding DJ, Bishop M, Cannings C. 2007, Chichester, Wiley, 945-979. full_text. 3

    Chapter  Google Scholar 

  9. Beaumont MA: Adaptation and speciation: what can F ST tell us. Trends Ecol Evol. 2005, 20 (8): 435-440. 10.1016/j.tree.2005.05.017.

    Article  PubMed  Google Scholar 

  10. Lande R: Neutral theory of quantitative genetic variance in an island model with local extinction and colonization. Evolution. 1992, 46 (2): 381-389. 10.2307/2409859.

    Article  Google Scholar 

  11. Whitlock MC: Neutral additive genetic variance in a metapopulation. Genet Res. 1999, 74 (3): 215-221. 10.1017/S0016672399004127.

    Article  CAS  PubMed  Google Scholar 

  12. Spitze K: Population structure in Daphnia obtusa: quantitative genetic and allozymic variation. Genetics. 1993, 135 (2): 367-374.

    PubMed Central  CAS  PubMed  Google Scholar 

  13. Merilä J, Crnokrak P: Comparison of genetic differentiation at marker loci and quantitative traits. J Evol Biol. 2001, 14 (6): 892-903. 10.1046/j.1420-9101.2001.00348.x.

    Article  Google Scholar 

  14. Balloux F, Brunner H, Lugon-Moulin N, Hausser J, Goudet J: Microsatellites can be misleading: an empirical and simulation study. Evolution. 2000, 54 (4): 1414-1422.

    Article  CAS  PubMed  Google Scholar 

  15. Hedrick PW: A standardized genetic differentiation measure. Evolution. 2005, 59 (8): 1633-1638.

    Article  CAS  PubMed  Google Scholar 

  16. Jost L: G ST and its relatives do not measure differentiation. Mol Ecol. 2008, 17 (18): 4015-4026. 10.1111/j.1365-294X.2008.03887.x.

    Article  PubMed  Google Scholar 

  17. Hedrick PW: Highly variable loci and their interpretation in evolution and conservation. Evolution. 1999, 53 (2): 313-318. 10.2307/2640768.

    Article  Google Scholar 

  18. Balloux F, Goudet J: Statistical properties of population differentiation estimators under stepwise mutation in a finite island model. Mol Ecol. 2002, 11: 771-783. 10.1046/j.1365-294X.2002.01474.x.

    Article  CAS  PubMed  Google Scholar 

  19. Kalinowski ST: Evolutionary and statistical properties of three genetic distances. Mol Ecol. 2002, 11 (8): 1263-1273. 10.1046/j.1365-294X.2002.01520.x.

    Article  PubMed  Google Scholar 

  20. Slatkin M: A Measure of Population Subdivision Based on Microsatellite Allele Frequencies. Genetics. 1995, 139 (1): 457-462.

    PubMed Central  CAS  PubMed  Google Scholar 

  21. Weir BS, Cockerham CC: Estimating F-statistics for the analysis of population structure. Evolution. 1984, 38 (6): 1358-1370. 10.2307/2408641.

    Article  Google Scholar 

  22. Mitchell-Olds T, Schmitt J: Genetic mechanisms and evolutionary significance of natural variation in Arabidopsis. Nature. 2006, 441 (7096): 947-952. 10.1038/nature04878.

    Article  CAS  PubMed  Google Scholar 

  23. Balloux F: A computer program for the simulation of population genetics. J Heredity. 2001, 92: 301-302. 10.1093/jhered/92.3.301.

    Article  CAS  Google Scholar 

  24. Neuenschwander S, Hospital F, Guillaume F, Goudet J: quantiNemo: an individual-based program to simulate quantitative traits with explicit genetic architecture in a dynamic metapopulation. Bioinformatics. 2008, 24 (13): 1552-1553. 10.1093/bioinformatics/btn219.

    Article  CAS  PubMed  Google Scholar 

  25. Bonnin I, Prosperi JM, Olivieri I: Genetic markers and quantitative genetic variation in Medicago truncatula (Leguminosae): a comparative analysis of population structure. Genetics. 1996, 143 (4): 1795-1805.

    PubMed Central  CAS  PubMed  Google Scholar 

  26. Hudson RR: Generating samples under a Wright-Fisher neutral model of genetic variation. Bioinformatics. 2002, 18 (2): 337-338. 10.1093/bioinformatics/18.2.337.

    Article  CAS  PubMed  Google Scholar 

  27. R Development Core Team: R: A language and environment for statistical computing. 2006, R Foundation for Statistical Computing, Vienna, Austria, [http://www.r-project.org]

    Google Scholar 

  28. Goudet J: FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). 2001, [http://www2.unil.ch/popgen/softwares/fstat.htm]

    Google Scholar 

  29. Kimmel M, Chakraborty R, King JP, Bamshad M, Watkins WS, Jorde LB: Signatures of Population Expansion in Microsatellite Repeat Data. Genetics. 1998, 148 (4): 1921-1930.

    PubMed Central  CAS  PubMed  Google Scholar 

  30. Roychoudhury A, Stephens M: Fast and Accurate Estimation of the Population-Scaled Mutation Rate, {theta}, From Microsatellite Genotype Data. Genetics. 2007, 176 (2): 1363-1366. 10.1534/genetics.105.049080.

    Article  PubMed Central  PubMed  Google Scholar 

  31. Goudet J: hierfstat, a package for R to compute and test hierarchical F-statistics. Mol Ecol Notes. 2005, 5 (1): 184-186. 10.1111/j.1471-8286.2004.00828.x.

    Article  Google Scholar 

  32. Meirmans PG: Using the AMOVA framework to estimate a standardized genetic differentiation measure. Evolution. 2006, 60 (11): 2399-2402.

    Article  PubMed  Google Scholar 

  33. Michalakis Y, Excoffier L: A generic estimation of population subdivision using distances between alleles with special reference for microsatellite loci. Genetics. 1996, 142 (3): 1061-1064.

    PubMed Central  CAS  PubMed  Google Scholar 

  34. Slatkin M: Inbreeding coefficients and coalescence times. Genet Res. 1991, 58: 167-175. 10.1017/S0016672300029827.

    Article  CAS  PubMed  Google Scholar 

  35. Hardy OJ, Charbonnel N, Freville H, Heuertz M: Microsatellite allele sizes: a simple test to assess their significance on genetic differentiation. Genetics. 2003, 163 (4): 1467-1482.

    PubMed Central  CAS  PubMed  Google Scholar 

  36. Hardy OJ, Vekemans X: SPAGeDi: a versatile computer program to analyse spatial genetic structure at the individual or population levels. Mol Ecol Notes. 2002, 2: 618-620. 10.1046/j.1471-8286.2002.00305.x.

    Article  Google Scholar 

  37. Pico FX, Mendez-Vigo B, Martinez-Zapater JM, Alonso-Blanco C: Natural Genetic Variation of Arabidopsis thaliana is Geographically Structured in the Iberian Peninsula. Genetics. 2008, 180: 1009-1021. 10.1534/genetics.108.089581.

    Article  PubMed Central  PubMed  Google Scholar 

  38. Le Corre V: Variation at two flowering time genes within and among populations of Arabidopsis thaliana: comparison with markers and traits. Mol Ecol. 2005, 14 (13): 4181-4192. 10.1111/j.1365-294X.2005.02722.x.

    Article  CAS  PubMed  Google Scholar 

  39. Warthmann N, Fitz J, Detlef W: MSQT for choosing SNP assays from multiple DNA alignments. Bioinformatics. 2007, 23 (20): 2784-2787. 10.1093/bioinformatics/btm428.

    Article  CAS  PubMed  Google Scholar 

  40. Nordborg M, Hu TT, Ishino Y, Jhaveri J, Toomajian C, Zheng H, Bakker E, Calabrese P, Gladstone J, Goyal R, et al: The pattern of polymorphism in Arabidopsis thaliana. PLoS Biol. 2005, 3 (7): e196-10.1371/journal.pbio.0030196.

    Article  PubMed Central  PubMed  Google Scholar 

  41. Jost L: D vs. G ST : Response to Heller and Siegismund (2009) and Ryman and Leimar (2009). Mol Ecol. 2009, 18 (10): 2088-2091. 10.1111/j.1365-294X.2009.04186.x.

    Article  Google Scholar 

  42. Sun JX, Mullikin JC, Patterson N, Reich DE: Microsatellites Are Molecular Clocks That Support Accurate Inferences about History. Mol Biol Evol. 2009, 26 (5): 1017-1027. 10.1093/molbev/msp025.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  43. Symonds VV, Lloyd AM: An analysis of microsatellite loci in Arabidopsis thaliana: mutational dynamics and application. Genetics. 2003, 165 (3): 1475-1488.

    PubMed Central  CAS  PubMed  Google Scholar 

  44. Ellegren H: Microsatellites: simple sequences with complex evolution. Nat Rev Genet. 2004, 5 (6): 435-445. 10.1038/nrg1348.

    Article  CAS  PubMed  Google Scholar 

  45. Calabrese P, Sainudiin R: Models of microsatellite evolution. Statistical methods in Molecular Evolution. Edited by: Nielsen R. 2005, New York: Springer, 289-305.

    Google Scholar 

  46. Bhargava A, Fuentes FF: Mutational dynamics of microsatellites. Molecular Biotechnology. 2010, 44: 250-266. 10.1007/s12033-009-9230-4.

    Article  CAS  PubMed  Google Scholar 

  47. Balloux F, Lugon-Moulin N: The estimation of population differentiation with microsatellite markers. Mol Ecol. 2002, 11 (2): 155-165. 10.1046/j.0962-1083.2001.01436.x.

    Article  PubMed  Google Scholar 

  48. Clark RM, Schweikert G, Toomajian C, Ossowski S, Zeller G, Shinn P, Warthmann N, Hu TT, Fu G, Hinds DA, et al: Common Sequence Polymorphisms Shaping Genetic Diversity in Arabidopsis thaliana. Science. 2007, 317 (5836): 338-342. 10.1126/science.1138632.

    Article  CAS  PubMed  Google Scholar 

  49. Beaumont MA, Nichols RA: Evaluating loci for use in the genetic analysis of population structure. Proc R Soc Lond B Biol Sci. 1996, 263: 1619-1626. 10.1098/rspb.1996.0237.

    Article  Google Scholar 

  50. Leinonen T, O'Hara RB, Cano JM, Merilä J: Comparative studies of quantitative trait and neutral marker divergence: a meta-analysis. J Evol Biol. 2008, 21 (1): 1-17.

    CAS  PubMed  Google Scholar 

  51. Lynch M: The rate of polygenic mutation. Genet Res. 1988, 51: 137-148. 10.1017/S0016672300024150.

    Article  CAS  PubMed  Google Scholar 

  52. Lynch M, Walsh B: Genetics and the analysis of quantitative traits. 1998, Sunderland: Sinauer Associates, Inc.

    Google Scholar 

  53. Schultz ST, Lynch M, Willis JH: Spontaneous deleterious mutation in Arabidopsis thaliana. Proc Nat Acad Sci USA. 1999, 96 (20): 11393-11398. 10.1073/pnas.96.20.11393.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  54. Orr HA: The genetic theory of adaptation: a brief history. Nat Rev Genet. 2005, 6 (2): 119-127. 10.1038/nrg1523.

    Article  CAS  PubMed  Google Scholar 

  55. Goudet J, Büchi L: The effects of dominance, regular inbreeding and sampling design on Q ST , an estimator of population differentiation for quantitative traits. Genetics. 2006, 172 (2): 1337-1347. 10.1534/genetics.105.050583.

    Article  PubMed Central  PubMed  Google Scholar 

  56. Goudet J, Martin G: Under neutrality, Q ST ≤ F ST when there is dominance in an island model. Genetics. 2007, 176: 1371-1374. 10.1534/genetics.106.067173.

    Article  PubMed Central  PubMed  Google Scholar 

  57. Miller JR, Wood BP, Hamilton MB: F ST and Q ST Under Neutrality. Genetics. 2008, 180 (2): 1023-1037. 10.1534/genetics.108.092031.

    Article  PubMed Central  PubMed  Google Scholar 

  58. Whitlock MC: Evolutionary inference from Q ST . Mol Ecol. 2008, 17 (8): 1885-1896. 10.1111/j.1365-294X.2008.03712.x.

    Article  PubMed  Google Scholar 

  59. Bakker EG, Stahl EA, Toomajian C, Nordborg M, Kreitman M, Bergelson J: Distribution of genetic variation within and among local populations of Arabidopsis thaliana over its species range. Mol Ecol. 2006, 15 (5): 1405-1418. 10.1111/j.1365-294X.2006.02884.x.

    Article  CAS  PubMed  Google Scholar 

  60. He F, Kang D, Ren Y, Qu LJ, Zhen Y, Gu H: Genetic diversity of the natural populations of Arabidopsis thaliana in China. Heredity. 2007, 99 (4): 423-431. 10.1038/sj.hdy.6801020.

    Article  CAS  PubMed  Google Scholar 

  61. Bergelson J, Stahl E, Dudek S, Kreitman M: Genetic Variation Within and Among Populations of Arabidopsis thaliana. Genetics. 1998, 148 (3): 1311-1323.

    PubMed Central  CAS  PubMed  Google Scholar 

  62. Beck JB, Schmuths H, Schaal BA: Native range genetic variation in Arabidopsis thaliana is strongly geographically structured and reflects Pleistocene glacial dynamics. Mol Ecol. 2008, 17 (3): 902-915.

    Article  CAS  PubMed  Google Scholar 

  63. Stenoien HK, Fenster CB, Tonteri A, Savolainen O: Genetic variability in natural populations of Arabidopsis thaliana in northern Europe. Mol Ecol. 2005, 14 (1): 137-148. 10.1111/j.1365-294X.2004.02359.x.

    Article  CAS  PubMed  Google Scholar 

  64. Muller MH, Leppälä J, Savolainen O: Genome-wide effects of postglacial colonization in Arabidopsis lyrata. Heredity. 2007, 100 (1): 47-58. 10.1038/sj.hdy.6801057.

    Article  PubMed  Google Scholar 

  65. Clauss MJ, Mitchell-Olds T: Population genetic structure of Arabidopsis lyrata in Europe. Mol Ecol. 2006, 15 (10): 2753-2766.

    Article  CAS  PubMed  Google Scholar 

  66. Carreras-Carbonell J, Macpherson E, Pascual M: Population structure within and between subspecies of the Mediterranean triplefin fish Tripterygion delaisi revealed by highly polymorphic microsatellite loci. Mol Ecol. 2006, 15: 3527-3539. 10.1111/j.1365-294X.2006.03003.x.

    Article  CAS  PubMed  Google Scholar 

  67. O'Reilly P, Canino M, Bailey K, Bentzen P: Inverse relationship between F ST and microsatellite polymorphism in the marine fish walleye pollock (Theragra chalcogramma): implications for resolving weak population structure. Mol Ecol. 2004, 13: 1799-1814. 10.1111/j.1365-294X.2004.02214.x.

    Article  PubMed  Google Scholar 

  68. Nordborg M, Donnelly P: The coalescent process with selfing. Genetics. 1997, 146 (3): 1185-1195.

    PubMed Central  CAS  PubMed  Google Scholar 

  69. Abbott RJ, Gomes MF: Population genetic structure and outcrossing rate of Arabidopsis thaliana (L.) Heynh. Heredity. 1989, 62: 411-418. 10.1038/hdy.1989.56.

    Article  Google Scholar 

  70. Hoffmann MH, Bremer M, Schneider K, Burger F, Stolle E, Moritz G: Flower Visitors in a Natural Population of Arabidopsis thaliana. Plant Biol. 2003, 5: 491-494. 10.1055/s-2003-44784.

    Article  Google Scholar 

Download references

Acknowledgements

We thank Valerie Le Corre for providing the French populations, Odd-Arne Rognli and Anna Monika Lewandowska for providing the Norwegian populations and Carlos Alonso-Blanco and Xavier Pico for providing the Spanish populations. We thank Sinead Collins, Maarten Koornneef, Maria Clauss and Marie-Hélène Muller for comments on an earlier version of the manuscript, Samuel Neuenschwander gave advice on using the program quantiNEMO. We also thank an anonymous reviewer for constructive comments. Funding was provided by Max Planck Gesellschaft and by a grant from DFG to JdM within the collaborative research network SFB680.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Juliette de Meaux.

Additional information

Authors' contributions

The study was conceived by IK and JdM, IK performed the experiments and analysed the data. OL contributed samples. The manuscript was written by IK with input from all other authors. All authors read and approved the final manuscript.

An erratum to this article is available at http://dx.doi.org/10.1186/1471-2156-11-88.

Electronic supplementary material

12863_2009_780_MOESM1_ESM.XLS

Additional file 1:Population information. An Excel file with supplementary tables for information on sampled populations. details about microsatellite and SNP loci and genotyping (XLS 29 KB)

12863_2009_780_MOESM2_ESM.XLS

Additional file 2:Microsatellite genotyping. An Excel file with the microsatellite genotyping information and primer sequences. (XLS 26 KB)

Additional file 3:SNP positions. An Excel file with the genomic positions of the SNPs used in this study. (XLS 22 KB)

12863_2009_780_MOESM4_ESM.XLS

Additional file 4:Genotypic data. An Excel file with the microsatellite and SNP genotypes of the lines used in this study. (XLS 624 KB)

12863_2009_780_MOESM5_ESM.PDF

Additional file 5:Computer simulations with selfing. A PDF file with a supplementary figure for results of computer simulations when the rate of self-fertilisation is set to 0.9. (PDF 176 KB)

12863_2009_780_MOESM6_ESM.PDF

Additional file 6:Computer simulations for Q ST with selfing. A PDF file with a supplementary figure for results of computer simulations for Q ST when rate of self-fertilisation is set to 0.9. (PDF 68 KB)

12863_2009_780_MOESM7_ESM.PDF

Additional file 7:SNP ascertainment bias. A PDF file with supplementary figure for SNP ascertainment bias in the four different geographic regions and the Nordborg dataset. (PDF 161 KB)

Authors’ original submitted files for images

Rights and permissions

Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and permissions

About this article

Cite this article

Kronholm, I., Loudet, O. & de Meaux, J. Influence of mutation rate on estimators of genetic differentiation - lessons from Arabidopsis thaliana. BMC Genet 11, 33 (2010). https://doi.org/10.1186/1471-2156-11-33

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/1471-2156-11-33

Keywords