A functional mutation at position -155 in porcine APOE promoter affects gene expression
- Shixin Li†2, 4,
- Hao Zhang†1, 2,
- Ping Gao1, 3,
- Zanmou Chen1,
- Chong Wang1, 2 and
- Jiaqi Li1, 2Email author
© Li et al; licensee BioMed Central Ltd. 2011
Received: 10 May 2010
Accepted: 9 May 2011
Published: 9 May 2011
Apolipoprotein E, a component of the plasma lipoproteins, plays an important role in the transport and metabolism of cholesterol and other lipids. Three single nucleotide polymorphisms (SNPs) -491A>T, -219T>G and +113G>C in the regulatory region of human apolipoprotein E gene (APOE) change the promoter activity and are associated with a wide variety of disorders including Alzheimer disease (AD). Functional SNPs in porcine APOE gene 5' regulatory region have not been explored.
We examined SNPs within this region (from -831 to +855), and the analysis revealed that the T>A SNP at position -155 among these three polymorphism sites (-440, -155, +501) was found to exert a marked influence on the transcription of the porcine APOE gene. Electrophoretic mobility shift assays showed that the binding affinity of oligonucletides containing the -155A to transcription factor(s) was stronger than that of the -155T. Transient transfection assays and quantitative real-time PCR results revealed that the -155T>A variant enhanced the activity of the APOE promoter and was associated with increased APOE mRNA levels in vivo.
These data suggest that the -155T>A mutation in the promoter region of the porcine APOE gene is an important functional variant. The results provided new insights into aspects of pig genetics and might also facilitate the application of pigs in biomedical studies addressing important human diseases.
Apolipoprotein E (apoE = protein; APOE = gene) is a component of lipoproteins, and thereby regulates lipoprotein metabolism; apoE also plays a key role in maintaining neuronal integrity [1–4]. Utermann et al.  were the first to identify three isoforms of human apoE, named E2, E3, and E4. The allele encoding apoE4 is a risk factor for atherosclerosis [6, 7], and AD [1, 8, 9]. In addition, humans with E4 allele responded to a lipid-lowering therapy poorly whereas those with E2 allele sensitively . There are four single nucleotide polymorphisms (SNPs) at -491, -427, -219 and +113 in human APOE promoter [11–13]. The base substitutions at -491A>T and -219T>G were found to alter promoter activity and transcription factor binding affinity [12, 14, 15]. The -491SNP and -219SNP were related to different plasma apoE , LDL and cholesterol concentrations , and the risks of atherosclerosis  and AD [14, 15]. Furthermore, +113SNP modulated lipid, lipoprotein concentrations and aortic atherosclerosis [11, 13]. However, there were inconsistent reports about APOE polymorphisms and coronary heart disease  or AD [19–21].
In pigs, APOE has been mapped to chromosome 6 [22, 23]. Porcine APOE is 4267 nucleotides in length, comprising of four exons and three introns, and a (CG)13 microsatellite located within intron 3 . Additionally, Brzozoeska et al.  studied the cDNA sequence of porcine APOE, and Kurył  described three isoforms of porcine apoE. Fan et al.  recently demonstrated that a SNP within intron 2 of porcine APOE was associated with body conformation traits. However, the functional SNPs in the 5' regulatory region of porcine APOE remain unclear.
To identify the functional SNPs in the 5' regulatory region of porcine APOE, mRNA expression levels and promoter activities associated with different genotypes were analyzed with quantitative RT-PCR (qRT-PCR) and transient transfection assays respectively, potential cis-acting elements surrounding the SNP were examined with electrophoretic mobility shift assays (EMSAs). Our results indicate that the -155 SNP modulates the expression level of porcine APOE.
Screening for SNPs in the APOE 5' regulatory region
Three overlapping fragments from -831 to +855 (1686 bp) were amplified. Three SNPs were identified in this region: -155T>A, -440G>A, and +501A>T.
Genotype frequencies of -155T>A SNP
Genotype frequencies of the porcine APOE -155 SNP in different populations.
Alternations of predicted transcription factor binding sites surrounding porcine APOE SNPs
The three polymorphic sites were examined with MatInspector http://www.genomatix.de . Results revealed that the mutation -155 T>A lost the transcription factor sites for GAGA, KLF6, PURα, KKLF, cKROX and MAZ, and gained sites for BKLF and CTCF (additional file 1), while the other two (-440G>A, and +501A>T) did not show changes (data not shown).
Alignment of porcine and human APOE promoter sequences
Effects of -155 mutation on APOE mRNA levels
The -155SNP effects on APOE transcription
Impact of the -155T>A mutation on DNA-protein interactions
This study identified a functional SNP in the 5' regulatory region of porcine APOE. The behaviours of the allelic forms of the reporter gene expression, real-time PCR and EMSA in our study strongly suggested that -155T>A allelic differences of the APOE transcription may be a consequence of differential binding ability of transcriptional factors or differential transcriptional factors bound to a wild type or a mutation type present in cells.
To distinguish potentially functional SNPs from nonfunctional SNPs, we focused on cis-acting elements based on the suggestion of Knight  and used a predictive program http://www.genomatix.de . The results indicated there were different transcription factors binding the cis-acting elements surrounding the -155SNP (additional file 1). The -155T contains a CCCTCCC motif that is known as the SP1 binding site , and SP1 has been implicated for its function in regulating AD-associated genes . Meanwhile, the mutation at porcine APOE -155 site still had SP1 binding ability.
The existence of multiple transcription factors binding the -155 region was further reinforced by incubation of a -155T/A DNA probe with nuclear extracts from porcine fetal fibroblasts forming DNA-protein complexes as demonstrated by EMSA. Importantly, competition EMSA using excess unlabelled probes revealed a difference in the binding affinities of promoter alleles: excess -155A DNA cold probe effectively abolished complex formation, whereas 10-fold excess -155T DNA cold probe failed to prevent labelled -155A probe-protein complex formation. From the above results, we could consider that the -155A promoter had a significantly higher affinity for binding factors than that for the -155T promoter. Interestingly, for human APOE -491A>T mutation, the -491A displayed an increased affinity for human hepatic nuclear proteins , while the -491A showed an opposite trend for rat pheochromocytoma and human SK-N-SH neuroblastoma nuclear proteins .
It was demonstrated, in transfected cells, that the cloned promoter containing the -155A variant was significantly stronger than that of the corresponding -155T variant at driving luciferase expression (6.7-fold higher expression, P < 0.01). The cloned promoter in this research did not contain either of the other two identified polymorphic sites (-440 and +501). The difference in transcription efficiency was confirmed in vivo, and APOE mRNA levels were 11 times higher in liver tissue from -155AA homozygous pigs than that in the tissue from -155TT homozygotes (P < 0.01). In the report of Maloney et al. , human -491A>T variant interacted with -219G>T because both mutations altered the variety and binding affinity of transcription factors. However, the -401SNP and +501SNP in porcine APOE were not expected to change the transcription factors, which implied that there may be no interactive effect between porcine APOE -155SNP and the other two SNPs.
All these data suggest that polymorphism at SNP-155 modulates transcription of the porcine APOE gene by affecting the ability of the APOE promoter to bind to trans-acting factors. It is notable that the -155 T>A substitution alters the CCCTCCC sequence to CCCACCC. The CACCC box is a well-described cis-acting transcription element that can serve as a binding site for widely distributed transcription-activating factors that act collaboratively with other regulatory proteins . There are a larger family of zinc finger transcription factors bound to CACCC box . Mutation of this box is thought to change binding and transactivation by transcription factors and therefore to decrease transcription levels [33–36].
Our results suggest that the -155A allele is associated with increased levels of APOE transcription in vivo. It seems probable that the -155A variant can elevate levels of apoE protein. We also report that the frequency of the -155AA genotypes is significantly under-represented, compared to -155TT and -155TA, in each of the four different pig populations analyzed. Although the explanation for this depletion is unknown, there are several considerations as follows.
Firstly, it was previously reported that apoE enhanced cell lipid homeostasis [17, 37] and could reduce the risk of heart disease [16, 38]. In humans, the concentration of apoE in plasma or brain varies according to APOE genotype in the order E2 > E3 > E4 , and the E4 allele is an established risk factor for AD. The -491A allele of human APOE is also a risk factor for AD, but this function is associated with higher levels of APOE transcription versus the -491T allele, and these effects are exerted independently of E4 . In the experiments reported here we found that the -155A allele was associated with significantly increased transcription of porcine APOE both in transfected cells and hepatic tissue. Higher apoE concentration lead to increased cytotoxicity , thus -155A allele may be harmful to porcine survival.
Secondly, some recessive genes that terminate fetal development are known to be located on swine chromosome SSC6 between SW855 and SW122 , a region that includes the porcine APOE gene [22, 40]. The affected gene(s) in the homozygous state can prevent embryo development after 9 days post-coitus when the spherical embryo grows to the filamentous form, leading to embryonic death due to implantation failure . This suggests that under-representation of -155AA could be a consequence of linkage disequilibrium between porcine APOE and other genes in the vicinity.
We have identified a functional SNP in the regulatory region of the porcine APOE gene that was associated with altered interactions with DNA-binding factors, marked differences in the activity of the APOE promoter and levels of APOE mRNA in vivo.
Screening for porcine APOE 5' regulatory SNPs
Primers for SNP detection in the porcine APOE 5'-regulatory region
PCR product length
Real-time quantitative PCR
Total RNA was extracted from the hepatic tissue of adult Dahuabai pig (-155TT, -155TA, -155AA, 6 animals of each of the three genotypes) with TRIzol reagent (Invitrogen). QRT-PCR analysis was performed using the ABI 7500 system (Applied Biosystems) (primers: 5'-CGCAGGATGCCGGACAGA-3' and 5'-CCTCCTGCACTTGGTCAGACA-3'). The gene expression levels were qualified relative to the expression of β-actin by the comparative CT method .
The DNA segment spanning the -268 to -11 region of porcine APOE and containing either the -155T or -155A variants was amplified with the primers 5'- CACGCGT AGTGGCATGGAGAAA-3' and 5'- ACTCGAG ACTCCTCCAGAACT -3', thereby creating the restriction sites of Mlu I and Xho I (underlined). Following restriction enzyme digestion the PCR products were inserted to vector pGL3-basic (Promega). The integrity of cloned sequences was confirmed by sequencing.
Transient transfection assays
Porcine fetal fibroblast cell line was established as described previously . Cells (1.5 × 106 per well) were seeded into 96-well plates and grown to 80-90% confluence, and transfected with APOE promoter/firefly luciferase reporter plasmids by Lipofectamine 2000 transfection reagent (Invitrogen). To control for transfection efficiency, cells were co-transfected with 2 ng of Renilla luciferase reporter plasmid (Promega). After 48 h of transfection, cells were lysed and assayed for promoter activity using the dual luciferase reporter assay system. Firefly luciferase activity was expressed relative to Renilla luciferase activity.
Electrophoretic mobility shift assays
Nuclear proteins were extracted from porcine fetal fibroblast cells cultured at third passage (Nuclear Extract Kit, Active Motif). The sequences used for EMSAs were 5'-CCCCTCCCCC(T / A ) CCCTGCTGTGTG-3' surrounding the -155 site. The oligonucleotides were 5'-labeled with [γ-32P]-ATP using a Megalabel DNA 5'-labelling Kit according to the specifications of the manufacturer (Takara). The 5'-labelled probes used in subsequent experiments were double stranded.
The binding mixture included 0.5 μg poly (dI-dC) (Amersham Biosciences), 2 μl of 5 × gel-shift binding buffer, 2 μg of nuclear extract. The mixture was maintained at room temperature for 10 min, 2 μl of radiolabelled oligonucleotide probe was added, and incubation was kept for a further 20 min at room temperature in the presence or absence of a 10- to 50-fold molar excess of unlabelled probes. DNA-protein complexes were fractionated by electrophoresis on 5% non-denaturating polyacrylamide gels.
For continuous data, comparisons of two means employed independent t tests, q values were calculated for comparisons of three means. Genotype frequencies were analyzed with probit models .
apoliprotein E gene
electrophoretic mobility shift assays
single nucleotide polymorphism
quantitative real-time polymerase chain reaction.
We wish to thank Changli Wu and Liangliang Wang for their help in PCR and animal genotyping.
The Work was supported by National Science Foundation of China (30771545, 30871781), the 863 Project of China (2007AA10Z161, 2008AA10Z135) and the earmarked fund for Modern Agro-industry Technology Research System (CARS-36).
- Mahley R, Huang Y: Apolipoprotein E: from atherosclerosis to Alzheimer's disease and beyond. Curr Opin Lipidol. 1999, 10 (3): 207-217. 10.1097/00041433-199906000-00003.PubMed
- Kim J, Basak JM, Holtzman DM: The role of apolipoprotein E in Alzheimer's disease. Neuron. 2009, 63 (3): 287-303. 10.1016/j.neuron.2009.06.026.PubMed CentralPubMed
- Mahley RW: Apolipoprotein E: cholesterol transport protein with expanding role in cell biology. Science. 1988, 240 (4852): 622-630. 10.1126/science.3283935.PubMed
- Rogers JT, Weeber EJ: Reelin and apoE actions on signal transduction, synaptic function and memory formation. Neuron Glia Biol. 2008, 4 (3): 259-270. 10.1017/S1740925X09990184.PubMed
- Utermann G, Langenbeck U, Beisiegel U, Weber W: Genetics of the apolipoprotein E system in man. Am J Hum Genet. 1980, 32 (3): 339-347.PubMed CentralPubMed
- Heeren J, Beisiegel U, Grewal T: Apolipoprotein E recycling: implications for dyslipidemia and atherosclerosis. Arterioscler Thromb Vasc Biol. 2006, 26 (3): 442-448.PubMed
- Kypreos KE, Karagiannides I, Fotiadou EH, Karavia EA, Brinkmeier MS, Giakoumi SM, Tsompanidi EM: Mechanisms of obesity and related pathologies: role of apolipoprotein E in the development of obesity. FEBS J. 2009, 276 (20): 5720-5728. 10.1111/j.1742-4658.2009.07301.x.PubMed
- Lehtimäki T, Pirttilä T, Mehta PD, Wisniewski HM, Frey H, Nikkari T: Apolipoprotein E (apoE) polymorphism and its influence on ApoE concentrations in the cerebrospinal fluid in Finnish patients with Alzheimer's disease. Hum Genet. 1995, 95 (1): 39-PubMed
- Ilveskoski E, Perola M, Lehtimaki T, Laippala P, Savolainen V, Pajarinen J, Penttila A, Lalu KH, Mannikko A, Liesto KK, Koivula T, Karhunen PJ: Age-dependent association of apolipoprotein E genotype with coronary and aortic atherosclerosis in middle-aged men: an autopsy study. Circulation. 1999, 100 (6): 608-613.PubMed
- Nieminen T, Kähönen M, Viiri L, Grönroos P, Lehtimäki T: Pharmacogenetics of apolipoprotein E gene during lipid-lowering. Pharmacogenomics. 2008, 9 (10): 1486-1745.
- Viiri LE, Loimaala A, Nenonen A, Islam S, Vuori I, Karhunen PJ, Lehtimaki T: The association of the apolipoprotein E gene promoter polymorphisms and haplotypes with serum lipid and lipoprotein concentrations. Atherosclerosis. 2005, 179 (1): 161-167. 10.1016/j.atherosclerosis.2004.10.004.PubMed
- Artiga MJ, Bullido MJ, Sastre I, Recuero M, Garcia MA, Aldudo J, Vazquez J, Valdivieso F: Allelic polymorphisms in the transcriptional regulatory region of apolipoprotein E gene. Febs Lett. 1998, 421 (2): 105-108. 10.1016/S0014-5793(97)01543-3.PubMed
- Viiri LE, Viiri KM, Ilveskoski E, Huhtala H, Maki M, Tienari PJ, Perola M, Lehtimaki T, Karhunen PJ: Interactions of functional apolipoprotein E gene promoter polymorphisms with smoking on aortic atherosclerosis. Circ Cardiovasc Genet. 2008, 1 (2): 107-116. 10.1161/CIRCGENETICS.108.791764.PubMed
- Bullido MJ, Artiga MJ, Recuero M, Sastre I, Carcía MA, Alaudo J, Lendon C, Han SW, Morris JC, Frank A, Várquez J, Goate A, Valdivivso F: A polymorphism in the regulatory region of APOE associated with risk for Alzheimer's dementia. Nat Genet. 1998, 18:
- Maloney B, Ge YW, Petersen RC, Hardy J, Rogers JT, Perez-Tur J, Lahiri DK: Functional characterization of three single-nucleotide polymorphisms present in the human APOE promoter sequence: Differential effects in neuronal cells and on DNA-protein interactions. Am J Med Genet B Neuropsychiatr Genet. 2010, 153B (1): 185-201.PubMed
- Lambert JC, Brousseau T, Defosse V, Evans A, Arveiler D, Ruidavets JB, Haas B, Cambou JP, Luc G, Ducimetiere P, Cambien F, Chartier-Harlin MC, Amouyel P: Independent association of an APOE gene promoter polymorphism with increased risk of myocardial infarction and decreased APOE plasma concentrations-the ECTIM study. Hum Mol Genet. 2000, 9 (1): 57-61. 10.1093/hmg/9.1.57.PubMed
- Viiri LE, Raitakari OT, Huhtala H, Kahonen M, Rontu R, Juonala M, Hutri-Kahonen N, Marniemi J, Viikari JS, Karhunen PJ, Lehtimaki T: Relations of APOE promoter polymorphisms to LDL cholesterol and markers of subclinical atherosclerosis in young adults. J Lipid Res. 2006, 47 (6): 1298-1306. 10.1194/jlr.M600033-JLR200.PubMed
- Ward H, Mitrou PN, Bowman R, Luben R, Wareham NJ, Khaw KT, Bingham S: APOE genotype, lipids, and coronary heart disease risk: a prospective population study. Arch Intern Med. 2009, 169 (15): 1424-1429. 10.1001/archinternmed.2009.234.PubMed
- Tycko B, Lee JH, Ciappa A, Saxena A, Li CM, Feng L, Arriaga A, Stern Y, Lantigua R, Shachter N, Mayeux R: APOE and APOC1 promoter polymorphisms and the risk of Alzheimer disease in African American and Caribbean Hispanic individuals. Arch Neurol. 2004, 61 (9): 1434-1439. 10.1001/archneur.61.9.1434.PubMed
- Thome J, Gewirtz JC, Sakai N, Zachariou V, Retz-Junginger P, Retz W, Duman RS, Rosler M: Polymorphisms of the human apolipoprotein E promoter and bleomycin hydrolase gene: risk factors for Alzheimer's dementia?. Neurosci Lett. 1999, 274 (1): 37-40. 10.1016/S0304-3940(99)00662-X.PubMed
- Zurutuza L, Verpillat P, Raux G, Hannequin D, Puel M, Belliard S, Michon A, Pothin Y, Camuzat A, Penet C, Martin C, Brice A, Campion D, Clerget-Darpoux F, Frebourg T: APOE promoter polymorphisms do not confer independent risk for Alzheimer's disease in a French population. Eur J Hum Genet. 2000, 8 (9): 713-716. 10.1038/sj.ejhg.5200513.PubMed
- Bosak N, Faraut T, Mikawa S, Uenishi H, Kiuchi S, Hiraiwa H, Hayashi T, Yasue H: Construction of a high-resolution comparative gene map between swine chromosome region 6q11-->q21 and human chromosome 19 q-arm by RH mapping of 51 genes. Cytogenet Genome Res. 2003, 102 (1-4): 109-115. 10.1159/000075734.PubMed
- Kurył J: Phenotyping of porcine Apoliprotein E using isoelectric focusing and localization of the apoE gene within the Halothane-susceptibility linkage group. Biochem Genet. 1999, 37 (9-10): 281-287.PubMed
- Ramsoondar JJ, Rucker EB, Vasquez JC, Gallagher DS, Grimm DR, Lunney JK, Schook LB, Piedrahita JA: Isolation and genetic characterization of the porcine apolipoprotein E gene. Anim Genet. 1998, 29 (1): 43-47. 10.1046/j.1365-2052.1998.00273.x.PubMed
- Brzozowska A, Grimholt U, Kulseth MA, Wold I, Rogne S: The sequence of porcine apolipoprotein E (APOE) cDNA. DNA Seq. 1993, 4 (3): 207-210.PubMed
- Fan B, Onteru SK, Mote BE, Serenius T, Stalder KJ, Rothschild MF: Large-scale association study for structural soundness and leg locomotion traits in the pig. Genet Sel Evol. 2009, 41: 14-10.1186/1297-9686-41-14.PubMed CentralPubMed
- Cartharius K, Frech K, Grote K, Klocke B, Haltmeier M, Klingenhoff A, Frisch M, Bayerlein M, Werner T: MatInspector and beyond: promoter analysis based on transcription factor binding sites. Bioinformatics. 2005, 21 (13): 2933-2942. 10.1093/bioinformatics/bti473.PubMed
- Knight JC: Regulatory polymorphisms underlying complex disease traits. J Mol Med. 2005, 83 (2): 97-109. 10.1007/s00109-004-0603-7.PubMed CentralPubMed
- Bockamp EO, Fordham JL, Gottgens B, Murrell AM, Sanchez MJ, Green AR: Transcriptional regulation of the stem cell leukemia gene by PU.1 and Elf-1. J Biol Chem. 1998, 273 (44): 29032-29042. 10.1074/jbc.273.44.29032.PubMed
- Dosunmu R, Wu J, Adwan L, Maloney B, Basha MR, McPherson CA, Harry GJ, Rice DC, Zawia NH, Lahiri DK: Lifespan profiles of Alzheimer's disease-associated genes and products in monkeys and mice. J Alzheimers Dis. 2009, 18 (1): 211-230.PubMed
- Hartzog G, Myers R: Discrimination among potential activators of the beta-globin CACCC element by correlation of binding and transcriptional properties. Mol Cell Biol. 1993, 13 (1): 44-56.PubMed CentralPubMed
- Wang X, Talamantez JL, Adamo ML: A CACCC box in the proximal exon 2 promoter of the rat insulin-like growth factor I gene is required for basal promoter activity. Endocrinology. 1998, 139 (3): 1054-1066. 10.1210/en.139.3.1054.PubMed
- Kulozik AE, Bellan-Koch A, Bail S, Kohne E, Kleihauer E: Thalassemia intermedia: moderate reduction of beta globin gene transcriptional activity by a novel mutation of the proximal CACCC promoter element. Blood. 1991, 77 (9): 2054-2058.PubMed
- Faustino P, Lavinha J, Marini MG, Moi P: beta-Thalassemia mutation at -90C-->T impairs the interaction of the proximal CACCC box with both erythroid and nonerythroid factors. Blood. 1996, 88 (8): 3248-3249.PubMed
- Feng WC, Southwood CM, Bieker JJ: Analyses of beta-thalassemia mutant DNA interactions with erythroid Kruppel-like factor (EKLF), an erythroid cell-specific transcription factor. J Biol Chem. 1994, 269 (2): 1493-1500.PubMed
- Moi P, Faa V, Marini MG, Asunis I, Ibba G, Cao A, Rosatelli MC: A novel silent beta-thalassemia mutation in the distal CACCC box affects the binding and responsiveness to EKLF. Br J Haematol. 2004, 126 (6): 881-884. 10.1111/j.1365-2141.2004.05146.x.PubMed
- Poirier J: Apolipoprotein E, cholesterol transport and synthesis in sporadic Alzheimer's disease. Neurobiol Aging. 2005, 26 (3): 355-361. 10.1016/j.neurobiolaging.2004.09.003.PubMed
- Hirashiki A, Yamada Y, Murase Y, Suzuki Y, Kataoka H, Morimoto Y, Tajika T, Murohara T, Yokota M: Association of gene polymorphisms with coronary artery disease in low- or high-risk subjects defined by conventional risk factors. J Am Coll Cardiol. 2003, 42 (8): 1429-1437. 10.1016/S0735-1097(03)01062-3.PubMed
- Miyata M, Smith JD: Apolipoprotein E allele-specific antioxidant activity and effects on cytotoxicity by oxidative insults and beta-amyloid peptides. Nat Genet. 1996, 14 (1): 55-61. 10.1038/ng0996-55.PubMed
- Mikawa S, Kishi H, Ogawa H, Iga K, Uenishi H, Yasue H: Analysis of recessive lethality on swine chromosome 6 in a Gottingen miniature resource family. Anim Genet. 2005, 36 (5): 376-380. 10.1111/j.1365-2052.2005.01322.x.PubMed
- Sambrook J, Russell DW: Molecular Cloning: A Laboratory Manual. 2001, NY: Cold Spring Harbor Laboratory, 3
- Schmittgen TD, Livak KJ: Analyzing real-time PCR data by the comparative CT method. Nature Protocols. 2008, 3 (6): 1101-1108. 10.1038/nprot.2008.73.PubMed
- Boquest AC, Day BN, Prather RS: Flow cytometric cell cycle analysis of cultured porcine fetal fibroblast cells. Biol Reprod. 1999, 60 (4): 1013-1019. 10.1095/biolreprod60.4.1013.PubMed
- Kaps M, Lamberson W: Biostatistics for animal science. 2004, Wallingford: CABI Publishing
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