Joint analysis of quantitative trait loci and major-effect causative mutations affecting meat quality and carcass composition traits in pigs
© Cherel et al; licensee BioMed Central Ltd. 2011
Received: 14 February 2011
Accepted: 29 August 2011
Published: 29 August 2011
Detection of quantitative trait loci (QTLs) affecting meat quality traits in pigs is crucial for the design of efficient marker-assisted selection programs and to initiate efforts toward the identification of underlying polymorphisms. The RYR1 and PRKAG3 causative mutations, originally identified from major effects on meat characteristics, can be used both as controls for an overall QTL detection strategy for diversely affected traits and as a scale for detected QTL effects. We report on a microsatellite-based QTL detection scan including all autosomes for pig meat quality and carcass composition traits in an F2 population of 1,000 females and barrows resulting from an intercross between a Pietrain and a Large White-Hampshire-Duroc synthetic sire line. Our QTL detection design allowed side-by-side comparison of the RYR1 and PRKAG3 mutation effects seen as QTLs when segregating at low frequencies (0.03-0.08), with independent QTL effects detected from most of the same population, excluding any carrier of these mutations.
Large QTL effects were detected in the absence of the RYR1 and PRKGA3 mutations, accounting for 12.7% of phenotypic variation in loin colour redness CIE-a* on SSC6 and 15% of phenotypic variation in glycolytic potential on SSC1. We detected 8 significant QTLs with effects on meat quality traits and 20 significant QTLs for carcass composition and growth traits under these conditions. In control analyses including mutation carriers, RYR1 and PRKAG3 mutations were detected as QTLs, from highly significant to suggestive, and explained 53% to 5% of the phenotypic variance according to the trait.
Our results suggest that part of muscle development and backfat thickness effects commonly attributed to the RYR1 mutation may be a consequence of linkage with independent QTLs affecting those traits. The proportion of variation explained by the most significant QTLs detected in this work is close to the influence of major-effect mutations on the least affected traits, but is one order of magnitude lower than effect on variance of traits primarily affected by these causative mutations. This suggests that uncovering physiological traits directly affected by genetic polymorphisms would be an appropriate approach for further characterization of QTLs.
Early research into the genetics of meat quality in pigs found causative mutations associated with marked effects on pig meat value, leading to worldwide applications in the pork production chain. The RYR1 Cys615 mutation  alters the function of a skeletal muscle sarcoplasmic calcium release channel, changes the contraction response to stimuli and gives rise to a malignant hyperthermia syndrome (MHS) on exposure to halothane gas. Characterization of this recessive phenotype first identified homozygous mutants, and subsequently the mutation itself. This mutation increases the pH fall rate in skeletal muscle post mortem and leads to the production of pale, soft, exsudative (PSE) meat, a long-recognized meat defect associated with malignant hyperthermia .
The PRKAG3 Gln200  mutation causes a dominant gain of function in adenosine monophosphate activated protein kinase (AMPK) complex regulating energy balance in skeletal muscle cells, which leads to an increased glycogen content of skeletal muscle tissue associated with an extended muscle pH fall post mortem and meat acidification. Measurements of muscle and meat glycogen concentrations, along with glycogen metabolites, were instrumental in the identification of mutation carriers and assisted the positional cloning of the causative mutation .
Outside these two causative mutations, phenotypic and genetic variation in meat quality traits is typically broad and seldom selected for, as pedigree record acquisition through the logistics of animal transport, slaughtering and meat appraisal is extremely costly. Selection efficiency is also limited by inaccurate estimation of breeding values for typical non-slaughtered selection candidates, as it relies solely on slaughtered relatives' phenotypes. With the benefits of genetic marker-assisted selection in mind, several studies of quantitative trait loci (QTLs) have dissected the genetic variation of meat quality traits in a range of swine populations, initially in exotic crosses to maximize segregating variation (Meishan, Erhualian and wild boar [4–8]) and then in more typical pig breeds used in pork production (Large-White, Duroc and Pietrain [9–16]).
We set out to revisit QTL detection of meat quality traits in the context of the genetic variation in commercial pig lines, purposely including in our reference population a limited proportion of RYR1 and PRKAG3 mutation carriers to directly compare examples of causative polymorphisms identified by QTL positional cloning, and newly detected QTLs.
Results and Discussion
Detection of eight significant QTLs contributing to genetic variation in meat quality traits
Meat quality, carcass and growth traits definition and summary
LL pH, 24-h post mortem.
SM pH, 24-h post mortem.
LL Glycolytic potential, 24 h
Loin pH, 45 min post mortem.
Lightness LL, 30-h post mortem
Red versus Green LL color
Yellow versus blue LL color
Shear force on cooked loin
Shear force on raw loin
Intramuscular fat %, LL
Loin drip losses, 48-h +4°C
Loin cooking yield, %
Lightness SM, 96-h post mortem
Red versus green SM color
Yellow versus blue SM color
Individual birth weight
Average daily gain 35-105 kg
Fat depth on ham cut section
Backfat depth, ultrasonic record
Loin area, ultrasonic record
Loin eye area, on sliced chop
Ham weight, including feet
Loin weight, including bones
Fat depth, back last rib, F.O.M.
Loin depth, back last rib, F.O.M.
Fat depth, lumbar; F.O.M.
Loin depth, lumbar, F.O.M.
Fat depth, back last rib, Probe
F- oP -L
Fat depth, lumbar, Probe
Fat depth, back, ruler on cut
F- HCr -L
Fat depth, lumbar, ruler on cut
Significant QTLs detected for meat quality, carcass composition and growth traits
Max (95% CI)1
QTL confirmed for meat colour on SSC6
We detected a significant QTL for loin meat colour parameters (redness, yellowness) on SSC6 in a confidence interval overlapping confidence intervals reported for a QTL detected on this chromosome for loin redness a* in several populations: half-sib families generated in a four-way cross using Pietrain × Large White composite/Large White F1 sires  a Duroc × Pietrain F2 population , and a Duroc × Large White F2 population . The QTL affecting a* in loin was the most significant of the meat quality QTLs detected in our study and explained 12% of the phenotypic variance. A QTL affecting the same trait, loin a* on SSC6 was also the most significant QTL reported from a QTL detection scan using a Japanese wild boar × Large White intercross population . In this case, the NUDT7 gene was subsequently proposed as a candidate gene underlying this QTL, and alleles of this gene were described as associated with different NUDT7 transcription levels . Recently, in vitro over-expression of this gene was shown to down-regulate heme synthesis, further substantiating claims for a biological mechanism leading to a meat colour change .
QTL confirmed for glycolytic potential and ultimate pH on SSC1
QTL detected for intramuscular fat percentage
We detected a significant QTL affecting intramuscular fat on the distal q arm of SSC5. A QTL has been reported for both intramuscular fat loin content and loin marbling on the same chromosome segment in an Erhualian × Duroc population .
A suggestive QTL (1% chromosome-wise significance) for intramuscular fat percentage was detected on SSC6 in a confidence interval overlapping those estimated for QTLs affecting the same trait in different F2 populations: Iberic × Landrace , Duroc × Landrace , Duroc × Pietrain  and Pietrain × Synthetic . If the QTLs detected in these different backgrounds are the same, then identified QTL alleles may be segregating in a large range of pig populations contributing to actual pork production and may reflect the lack of selection for this trait in many pig breeding programs, or antagonistic effects on other traits.
A third QTL affecting the same trait was detected on SSC7. Neighbouring locations on chromosome 7 were also suggested to contribute to genetic variation of this trait in a Duroc × Meishan F2 population  and in a Large White × Meishan F2 population . This QTL was subsequently not confirmed as segregating within a purebred Duroc population . The most likely location of this QTL in our study is between markers Sw2155 and LRA1, very close to the most likely location reported in the Large White × Meishan F2 population , but somewhere distant from the distal location proposed in the study of a Duroc × Meishan population . Our population showed no contribution from Meishan alleles but may share Large White alleles with the Large White × Meishan study  or Duroc alleles with the Duroc × Meishan F2 population .
QTL segregation for post mortem pH fall rate
We detected a suggestive QTL (1% chromosome-wise significance) on SSC12 affecting loin pH at 45 minutes post mortem between markers FH1993 and Sw957, where a QTL was detected for pH fall between 45 minutes and 3 hours post mortem in a Hampshire × Landrace cross . This result may point to segregation of Hampshire breed derived alleles in both of these populations.
Detection of 20 significant QTLs affecting carcass composition and growth traits
We detected 20 QTLs significant at a 5% genome-wise level for 16 carcass composition and growth traits analyzed and listed in Table 1. These significant QTLs explained 5.7-17.6% of phenotypic variance in our dataset. Significant QTLs were detected for fat thickness traits (11 QTLs), muscle development traits (7 QTLs) and birth weight (1 QTL). For completeness, we also report 49 suggestive QTLs for carcass and growth traits, significant at a 1% or 5% chromosome-wise threshold. A genome position-sorted list of significant QTL is presented in Table 2. A genome position-sorted list of suggestive QTL is presented in Additional File 1. These results point to several colocalized QTLs affecting fat deposition (5 QTLs on SSC5) or muscle development (4 QTLs on SSC11 and 4 QTLs on SSC13), suggesting pleiotropic effects of common determinants. Overlaid LRT profiles for detection of QTLs affecting these groups of traits on chromosomes 5, 11, and 13 are reported in Additional File 2.
A significant QTL was detected for birth weight on SSC10, where a QTL affecting the same trait was also reported at a similar position in a Duroc × Large White population . This independent confirmation is noteworthy considering the low number of QTLs reported for this trait, and this result points to major-effect alleles (10% phenotypic variance in our population) segregating from Duroc and/or Large White breeds.
PRKAG3 Gln200 mutation detected as a QTL on SSC15
The causative major-effect PRKAG3 Gln200 allele was detected as an extremely significant QTL explaining 53% of phenotypic variation in loin glycolytic potential in a control population including 3% of the mutated allele. Long-range linkage in this F2 population generates a significant QTL statistical test on most of SSC15. Highly significant QTLs were also detected at the same position for ultimate pH values in semimbranosus and longissimus muscles but not for pH-45, cooked loin shear force or intramuscular fat (Additional File 3). While QTL detection statistic at the PRKGA3 position on cooked loin shear force might be too low to reach significance thresholds in this population, the lack of any QTL detection affecting IMF or pH-45 suggests that previously reported significant genotype effects on these traits in association studies performed using this same dataset  could result from an association between genotype classes and undocumented class effects. Alternatively, the analysis of within-family segregation based on described IBD may have less power than population-wide comparison of genotype effects.
RYR1 Cys615 mutation detected as a QTL on SSC6
QTLs were also detected in this control population at the same or neighbouring locations (Figure 3) for a backfat thickness trait (F-op-B) and a loin muscle development trait (LMA-us). These results would fit generally accepted effects for the RYR1 mutation on carcass composition traits . These apparent genotype effects have also been verified in this same dataset using a model of analysis fitting RYR1 genotypes as fixed effects in addition to a polygenic additive effect . However, the exclusion of RYR1 mutation carriers from the QTL analysis does not stop detection of significant QTLs for those two carcass composition traits at the same chromosome locations (Figure 3, Additional File 3). For each of those two traits, our result suggests that in addition to the RYR1 mutation, an independent QTL located at a neighbouring location is segregating in this population. We also observed persisting suggestive or significant QTLs in the absence of the RYR1 mutation at similar positions on SSC6 when analyzing a related fatness trait (F-us) and a related loin muscle development trait (Loin-W), as summarized in Additional File 3.
Known causative mutations in QTL detection analyses
Segregation of known mutations was readily detected as highly significant QTLs at the genomic locations of the causative polymorphisms, using a population including a low frequency of mutant allele (p = 0.03-0.08) but using marker genotypes fully informative for causative mutations. This outcome was verified with pH-45 for RYR1 and glycolytic potential for PRKAG3, measurements of muscle physiology known to be directly affected by these gene functions. These results not only act as quality controls for record acquisition, genotyping procedures and statistical analysis but also illustrate the fact that the practical power to detect a major-effect haplotype in such an experiment is very high, provided genotypes are informative for QTL genotypes. QTL contribution to trait variance as reported here is a function of allele substitution effects, which were not estimated in this work (QTLs were modelled here as random effects, allowing fitting of any allelic series), and allele frequencies, which are population-dependent. The overall impact of RYR1 and PRKAG3 mutations in this dataset is obviously the result of very large allele substitution effects, as frequency of mutated alleles are known and relatively low (p = 0.03-0.08). Other QTLs detected in this work and showing a substantial impact on trait variance may result from smaller effects of more frequent alleles. Overall, these results point to the absence of any additional major-effect polymorphism affecting the same set of traits in this population, beyond the known RYR1 and PRKAG3 mutations. The very large effects of the RYR1 and PRKAG3 mutations can be seen as a consequence of the specific nature of these two polymorphisms, which are both non-conservative changes in the sequence of proteins encoded by these genes. However, even using these best possible conditions, we also observed that those major-effect mutations were detected as more typical significant or suggestive QTLs when analyzing less severely affected traits. Trait exposure to QTL effects appears to define opportunities for unambiguous QTL detection and ultimately QTL genotype prediction, as illustrated in Figure 4 for QTL genotypic values predicted from the pH-45 QTL analysis.
Whereas subtraction of PRKAG3 Gln200 carriers from the control population effectively suppressed QTL detection on SSC15 for all affected traits (Figure 2), we uncovered a complex picture of genetic effects segregating on SSC6 (Figure 3). The RYR1 mutation carriers contribute to but cannot fully explain QTL segregation for backfat thickness, while exclusion or inclusion of RYR1 Cys615 carriers does not interfere with QTLs detected for loin muscle section area. These results suggest that at least one extra polymorphism is segregating in this population in addition to the RYR1 mutation that affects backfat thickness. The RYR1-mutated animals contribute to the backfat QTL detection, but so do others. The detection of QTLs affecting loin muscle section area essentially takes no contribution from RYR1 mutation carriers, which might be interpreted as a fixation of either allele in families segregating for RYR1 alleles, or limited linkage disequilibrium of the two polymorphisms. The different contributions of RYR1 mutation carriers to backfat thickness and loin muscle area QTL detection on SSC6 as well as the different positions for most likely QTL locations (Figure 3, after exclusion of mutation carriers) further suggests that backfat thickness and loin muscle area QTLs could be driven by two independent polymorphisms in addition to the RYR1 mutation. QTLs affecting backfat thickness and loin muscle development located in overlapping SSC6 intervals had been reported previously in populations free of RYR1 mutation, further supporting this interpretation: a Duroc × Pietrain F2 population  and an Iberian × Landrace F2 population . A similar observation was made using a different approach in an F2 population including carriers of the RYR1 Cys615 mutation when analyzing QTL segregation on SSC6 after the correction of phenotypes for RYR1 mutation effects .
The RYR1 Cys615 mutation was fixed in the Pietrain breed and showed a high frequency in heavily muscled pig breeds  intensively selected for low backfat thickness and increased muscle development. These breeding programs may have contributed to the building of SSC6 haplotypes combining the RYR1 mutation and QTL alleles reducing backfat thickness and increasing muscle development, detected as a combined effect [29, 32].
Identification of the RYR1 gene as causative for MHS initially raised questions regarding the likelihood of physiological effects of the RYR1 Cys615 mutation on fat deposition, and suggested neighbouring functional candidate genes (APOE, TGF-β1, LIPE) as causative for those effects . A later study using a wild boar × domestic pig F2 population found an absence of QTL linked to the RYR1 mutation . Our data provide evidence for such linked QTL and show that assessing pleiotropic versus linked effects in F2 populations is restricted to the availability of observable recombinations between causative polymorphisms in the population under study. Recombined haplotypes that we detected in this work are unlikely to be the product of the F2 intercross design we used, but were more likely sampled from the Pietrain parental population where the RYR1 mutation was segregating at a low frequency (p < 0.20). These recombined haplotypes may be more common in contemporary pig sire lines as a result of breeding practices limiting the frequency of the RYR1 mutated allele (owing to its detrimental effects on meat quality), while selecting for decreased backfat and increased muscle development. This observation also suggests that re-estimating the supposedly favourable effects of the RYR1 Cys615 mutation on carcass composition within different purebred pig populations where it segregated for a sufficiently large number of generations would be more appropriate for a bias-free estimation of the mutation causative effects. Re-evaluating less favourable effects of this mutation on carcass composition traits would encourage pig breeders to eradicate the mutant allele in most contexts considering the unfavourable effects of the RYR1 Cys615 mutation on meat quality .
The occurrence of multiple linked QTLs has been commonly described as underlying the segregation of genetic effects in the pig , mouse [35, 36] and tomato . Successive removal of major-effect loci has been proposed as an explicit strategy in yeast  to further characterize alternative sources of genetic variation from the same given chromosome segment. Our observations incidentally support the relevance of this strategy and illustrate the specific opportunities of incomplete linkage arising from recombination within outbred livestock populations.
QTLs were identified for nearly all the meat quality and carcass composition traits analyzed, but more QTLs were detected for fat deposition, muscle development, and meat colour.
The interpretation of causative mutation control detection in addition to the detection of independent QTLs at neighbouring loci affecting the same traits suggests that part of the muscle development and backfat thickness effects that have been commonly associated with the RYR1 mutation [39, 40] results from linkage with neighbouring QTLs affecting the same traits.
Four to twenty percent of phenotypic variance is explained by each of the newly detected QTLs in this study, which remains within the range of major-effect mutation effects on traits not directly affected by RYR1 and PRKAG3 mutations (Drip-L/RYR1 5%; LL-L*/PRKAG3 19%). Considering the large impact achieved by control of the RYR1 and PRKAG3 mutations on pork production worldwide, this direct comparison suggests that QTLs detected in this study may also drive a valuable proportion of the variation in pork production, thereby encouraging the identification of underlying polymorphisms.
Conversely, the very large effects of the RYR1 and PRKAG3 mutations on pH-45 and glycolytic potential demonstrate the efficiency of QTL analysis in discriminating QTL genotypes. This observation suggests that the identification of physiological traits directly affected by the segregation of detected QTL, such as the muscle glycogenic potential measurement  would be key in QTL genotype prediction and hence in the dissection of underlying causative polymorphisms. This conclusion calls for a systematic search of primarily affected traits as the most informative ones for predicting unknown QTL genotypes. Both candidate biochemical traits and measurements derived from functional genomic screens or metabolomic profiles are relevant here.
An F2 population of females and barrows was produced from an intercross between two production sire lines: FH016 (Pietrain type, France Hybrides, St-Jean-de-Braye, France) and FH019 (Synthetic line from Duroc, Hampshire and Large White founders, France Hybrides). Eighteen F1 males and 72 F1 females were produced from 16 FH016 F0 males and 25 FH019 F0 females. F2 animals (1,370) were produced by mating each of the 18 F1 boars to the same group of one to four full sib F1 sows for the production of one to three litters. All animals were raised on the same farm and were slaughtered in the same abattoir in 32 successive batches. The number of records collected on F2 animals ranged from 700 to 1,350 according to the trait (Table 1 and Additional File 3).
Genomic DNA was extracted from white blood cell samples from F0 and F1 animals using a salting-out procedure  and from piglet tails docked at birth for F2 animals using a DNeasy tissue extraction kit (QIAGEN, Courtabeuf, France). One hundred and seventy microsatellite loci spanning all autosomes with an average spacing of 17 cM were selected based on predicted information content of available markers in this population. Information content was assessed from the number of alleles observed and average heterozygosity when genotyping all F1 males (data not shown). Microsatellites were genotyped by PCR and electrophoretic sizing of the PCR products. The sequence of primers used in PCR followed the reference pig genetic maps (, NCBI Map viewer Sscrofa5) or are summarized in Additional File 4 for complementary markers. Amplification was carried out with fluorescently labelled primers (M13 tag-tailed primer and labelled M13 primer in the same PCR reaction, Dyes IRD700/IRD800, Sigma-Genosys, Lyon, France). PCR was followed by pooling markers across different size ranges and a denaturing polyacrylamide gel electrophoresis revealed on a LICOR 4200 automated sequencer (Li-COR Inc., NE). Microsatellite alleles were called by PCR fragment sizing and binning using Li-COR SAGA genotyping software and a custom set of co-migrating size standards (fluorescently labelled PCR products from a set of plasmid inserts). We separated F1 parents and F2 progeny segregating alleles on common gels to allow a direct match between parents and progeny alleles. All gels were manually reviewed and edited for the assessment of genotyping quality and genotype validation. In addition to microsatellite genotypes, the MC4R and PRKAG3 V199I RFLP polymorphisms were genotyped as described [44, 45] and used as genetic markers. All F2, F1 and F0 animals were genotyped for all markers. We performed iterative Mendelian inheritance checks for each marker allele throughout the whole pedigree using the peeling algorithm implemented in the LOKI software package .
Multipoint linkage maps were constructed ab initio using CRIMAP 2.4 software . Genetic maps used for QTL detection were built from all the genotypes available from this population including carriers of the RYR1 and PRKAG3 mutations using the CRIMAP build option. However, the order of the closest markers was fixed as inferred from physical maps [48, 49] when a limited number of recombination events in this population did not allow proper assessment of marker order. The sex-averaged genetic maps used subsequently in IBD coefficient estimation and QTL detection analyses are reported in Additional File 4.
RYR1 and PRKAG3 genotypes
All F2 animals were individually genotyped for the RYR1 R615C and PRKAG3 R200Q mutations using previously described RFLP tests [3, 50]. These genotypes were used to define a population excluding carriers of either of the mutations we used for de novo QTL detection, while a control population included all the animals and was used for control detection of known mutations (mutant allele frequencies p RYR1 Cys615 = 0.08; p PRKAG3 Gln200 = 0.03). Additional File 3 lists the number of records per trait and per genotype analyzed in this control population including all animals or selectively removing mutation carriers. Mutation genotypes were subsequently included in the genetic marker genotypes used in QTL detection procedures, although they were only informative in the control population.
Meat quality traits
Early pH fall in loin muscle was recorded as pH at 45 minutes in the loin (pH-45) and was measured from a solution of 1 g of longissimus lumborum muscle dispersed 45 minutes post mortem in 9 ml of a 5 mM sodium iodoacetate - 150 mM potassium chloride buffer. Ultimate meat pH was recorded in loin (longissimus lumborum muscle, pH-LL) and ham (semimembranosus muscle, pH-SM) 24 h post mortem on half-carcasses. Intramuscular fat content (IMF) was measured as percent lipid (lipid weight:meat weight) as determined by chloroform/methanol extraction from freeze-dried longissimus lumborum sampled 30 h post mortem. Glycolytic potential (Glyc-P) was measured on the same longissimus lumborum samples and calculated from the enzymatic quantification of glycogen, glucose-6P and lactate concentrations .
Loins were sliced 30 hours post mortem, and two 3-4 cm slices (326 g +/- 65 g; including 11th-12th ribs) were collected. A first chop was used for loin colour measurement, raw meat shear force measurements and chemical analysis. The other chop was kept at 4°C for 48 h in a closed polyethylene plastic bag and then grill-cooked in a 240°C dry oven for 30 min. Drip loss was calculated as 100(1 − weight after storage/weight before storage). The same chop was weighed again after cooking and dripping and loin cooking yield was calculated as 100(weight after cooking/weight after storage). Raw meat and cooked loin instrumental tenderness were assessed by the measurement of shear force (Warner-Bratzler 60° cell, maximum strength over shearing of 1 cm diameter meat cylinders, average of ten repetitions). Loin lightness (CIE L*), redness (CIE a*), and yellowness (CIE b*) colorimetric parameters were recorded using a Minolta CR300 colorimeter (Konica Minolta Sensing Europe BV, Roissy, France). CIE L*, a* and b* colour measurements were acquired with the same equipment from deboned semimembranosus muscle 96 h post mortem. An in-depth account of procedures used for recording meat quality traits was reported previously [51, 52].
Carcass composition and growth traits
Backfat thickness and loin muscle cross-section area were recorded on live pigs at 105 kg bodyweight from echographic pictures of loin sections at the last rib level acquired using a 3.5 MHz linear transducer probe and an Aloka 500-V echograph (Aloka, Tokyo, Japan). Fat thickness and muscle depth were recorded from carcasses at slaughtering using the carcass grading system in use at the abattoir, Fat-O-Meter (SFK, Herlev, Denmark). Fat depth and muscle depth from this equipment are calculated from readings of an optical probe inserted through the skin, the back fat layers and the loin muscle and recording penetration depths at transitions between fat (white) and muscle (red) layers. Records were acquired at a dorsal (last rib) and a lumbar position, on a lateral line located 10 cm from the mid-line. A hand-operated optical probe (Introvison Ltd, Hitchin, UK) used on carcasses 24 h post mortem allowed a direct visual score of fat depths at similar locations. Fat depth was also recorded with a ruler on ham cuts directly above the humerus and on half-carcass mid-line cuts at a dorsal and lumbar position. The primal cut weights of ham without feet or loin but with bones were recorded at carcass cutting. Loin rib eye area was measured from digitized pictures of sliced chop. Individual birth weight was recorded on the first day of life and growth average daily gain was calculated as total weight gain during the ad libitum fed growth period (35 kg to 105 kg) divided by the number of days for this period. Elementary statistics for all traits analyzed along with abbreviations used and short definitions are presented in Table 1 for the F2 population excluding carriers of RYR1 and PRKAG3 mutations.
The genetic variability of the population used in this study, where phenotypes were recorded in a single F2 generation, can be described as a segregation of F1 alleles and can be analyzed by interval mapping for QTL segregation within each of half-sib or full-sib F2 families . However, parental haplotypes from these outbred populations cannot be treated as fixed in each parent population, and actual sizes of half-sib or full-sib families in our F2 population limits the detection power of within-family analyses or restrict analysis to the largest families. However, additional relationships exist in our pedigree beyond segregation within full-sib and half-sib families as F1 males and females used in different half-sib families were selected from the same F0 litters. Additionally, as all F1 females mated to the same F1 male were full sibs, relationships between F2 half sibs include sharing of identical F0 alleles transmitted by the different F1 females in addition to the segregation of paternal alleles. Use of sampling-based approaches associated with pedigree peeling makes it possible to infer sharing of identical-by-descent (IBD) founder alleles among all phenotyped (F2) animals .
We present results from a QTL detection analysis based on population-wide analyses of covariance between relatives associated with their IBD coefficients for each particular genomic position considered, as could be inferred from pedigree and inheritance of linked marker alleles. QTL detection was performed using a two-step variance component estimation method as proposed by George  and subsequently demonstrated in livestock [9, 55]. Briefly, univariate mixed models of variance were fitted to trait observations under either an additive polygenic model (H0) or a QTL model (H1: additive polygenic effect and QTL effect) for each of the tested positions. Both variance analysis models included the same fixed effects describing slaughter or fattening batches (all traits) and sex (carcass composition traits, cooked loin shear force, glycolytic potential and intramuscular fat percentage). Carcass weight or live weight was used as a covariate in all carcass composition trait models. A covariate for chop weight was used in the loin cooking yield and cooked loin shear force models. An independent random effect was used to fit a common environment defined as birth in a common litter in the analysis of individual birth weight and average daily gain. Additive genetic effect was set up using a three-generation pedigree structure in addition to the phenotyped animals, and QTL effects were fitted using the IBD relationship matrix estimated for the given genome position. IBD relationship matrices were estimated using a reversible jump Monte Carlo Markov chain method every 4 cM along linkage groups using the software package LOKI 2.4.6  and sampling over 20,000 iterations. No assumptions were made regarding IBD status of founder alleles originating from the same parental line, i.e. all IBD probabilities of founder alleles were set to 0, irrespectively of founder parental line origin. This was set to describe expected allele heterogeneity within these outbred parental lines, with known admixture history (one of the two parental lines being a synthetic line). Variance components were estimated using a residual maximum likelihood (REML) method with ASREML 2.0 software .
A QTL detection test was computed at each of the scanned positions using a likelihood ratio test (LRT) of −2(logikelihood-H1 − loglikelihood-H0).
It was suggested that this statistic followed a chi-squared distribution from 1 DF (for one specific position) to 2 DF when testing over a genetic interval . The maximum LRT recorded for each scanned chromosome was compared with chromosome-wise thresholds obtained by simulation (see below) to assess QTL detection. The sum of estimated variance components as estimated from a polygenic additive model (H0) in a population excluding RYR1 and PRKAG3 mutation carriers was used to compute reference trait standard deviation in summary statistics of Table 1. The proportion of variance explained by each QTL effect was estimated as the ratio between the variance component associated with the IBD relationship matrix at most significant QTL position and the sum of variance components estimated from the same model (Additional File 5). In both cases, variance estimates refer to total phenotypic variance after correction for fixed effects and covariates used.
We checked total variance inflation or deflation between significant QTL models and polygenic (null hypothesis) models. Out of the significant and suggestive QTLs reported, we noted five cases where QTL model total variance deviated from additive model total variance by more than 2% (namely QTL for LL-a* (+5%), LL-b* (+3%) on SSC6, QTL for ADG on SSC1 (+3%) and QTL for F-FOM-B on SSC3 (−3%) and SSC5 (−4%)). In all other significant and suggestive QTL models, the sum of variance components was within 2% of the sum of variance components estimated under a polygenic model. We checked the sampling error of estimated QTL variance component, as estimated from the square root of the diagonal element of the average information matrix . For all significant QTLs, this sampling error reported as a proportion of estimated variance component (component/sampling error ratio), ranged from 1.73 to 2.82 over 28 significant QTLs, with an average value of 2.07.
Significance thresholds and confidence intervals
We inferred chromosome-wise significance thresholds from the distribution of QTL detection statistics observed on phenotypes simulated under the null hypothesis. We ran QTL detection using the same marker genotypes (IBD coefficients) and pedigree on sets of simulated phenotypes modelled from our pedigree structure as carrying polygenic additive variation only (h2 = 0.3). However, to save computing, we ran QTL autosome scans on a large number (5,000) of simulated phenotype datasets but using a smaller subset of animals (305 animals; 4 half-sib families). We also scanned QTLs on four representative test chromosomes (SSC1, SSC6, SSC15, and SSC18) over fewer (1,000) full-sized simulated datasets modelled from the population structure used for a typical meat quality trait record (SF-cook, n = 760). A summary of selected quantile values from the distribution of maximum LRT detected on these datasets simulated under the null hypothesis is tabulated for each chromosome in Additional File 6. Chromosome-wise significance thresholds were found to vary to some extent according to linkage group size, but were not influenced by dataset size (Additional File 6), consistent with the expected distribution of nominal test statistic. Empirical simulation-based chromosome-wise thresholds were found to be close to corresponding quantiles from a χ2 2DF distribution, the upper bound theoretical estimate proposed for a genetic interval  (1% quantiles were found ranging from 7.7 to 9.5 over the 18 autosomes, compared with a 1% quantile from χ2 2DF of 9.21).
Simulations were performed only once and quantiles drawn from the null hypothesis LRT distribution of the largest set of simulations (5,000) using small datasets for each chromosome were used as chromosome-specific chromosome-wise significance thresholds throughout all traits analyzed in this work, irrespective of the actual number of records in each analysis. We considered only maximum QTL detection statistics across all positions along each linkage group against these chromosome-wise thresholds without considering any secondary peaks. Genome-wise significance levels were adjusted using a Bonferroni correction as suggested previously , and QTLs are reported as significant for a 5% genome-wise significance level (equivalent to a 0.285% chromosome-wise level for independent scanning of 18 autosomes per genome). QTLs detected with an LRT above 1% or 5% chromosome-wise threshold but below the 5% genome-wise threshold are reported as suggestive QTLs. A 1% genome-wise threshold is proposed to support interpretation of the most significant QTL detection tests in figures and tables as drawn from a χ2 2DF distribution and using the same Bonferroni correction for the testing of 18 chromosomes (1% genome-wise threshold corresponding to a 0.058% chromosome-wise threshold).
Confidence intervals are proposed on the basis of a -1 LOD drop-off and are reported as 95% confidence interval map segments where the loglikelihood of the QTL model is higher than loglikelihood (max) − ln(10) . Predicted QTL genotypic effects were obtained from solutions of mixed model equations for QTL models fitted at most significant chromosome position defined by maximum LRT.
Comparison of results with published QTLs
We identified parallels between significant QTLs detected in this study and QTLs reported in the literature for similar traits by searching the online directory of published QTLs, the pig section of the AnimalQTLdb release 12 . However, in most cases, the very large number of QTLs (5,986) described in pigs, and the very large confidence intervals associated with F2 and backcross studies, prevent formal identity confirmations with our detected QTLs.
The authors thank Jean-François Nicolaon and the staff from the France Hybrides nucleus herd of Sichamps for experimental animal care and identification, Rachel Marie, Laurent Letelu, Caroline Cordebois and Najate Aït-Ali for excellent technical assistance, and the Orléans-Viandes abattoir for their assistance in tracking animals and carcasses. This work had the financial support of the Ministère de la Recherche, Appel à projets Post Séquençage Génomique, D116 and the ANR-Agence Nationale de la Recherche - French National Research Agency, under the Programme National de Recherches en Alimentation et nutrition humaine project ANR-06-PNRA-025, GENMASCQ.
- Fujii J, Otsu K, Zorzato F, de LS, Khanna VK, Weiler JE, O'Brien PJ, MacLennan DH: Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia. Science. 1991, 253: 448-451. 10.1126/science.1862346.View ArticlePubMedGoogle Scholar
- Pommier SA, Houde A: Effect of the genotype for malignant hyperthermia as determined by a restriction endonuclease assay on the quality characteristics of commercial pork loins. J Anim Sci. 1993, 71: 420-425.PubMedGoogle Scholar
- Milan D, Jeon JT, Looft C, Amarger V, Robic A, Thelander M, Rogel-Gaillard C, Paul S, Iannuccelli N, Rask L: A mutation in PRKAG3 associated with excess glycogen content in pig skeletal muscle. Science. 2000, 288: 1248-1251. 10.1126/science.288.5469.1248.View ArticlePubMedGoogle Scholar
- Andersson-Eklund L, Marklund L, Lundstrom K, Haley CS, Andersson K, Hansson I, Moller M, Andersson L: Mapping quantitative trait loci for carcass and meat quality traits in a wild boar × Large White intercross. J Anim Sci. 1998, 76: 694-700.PubMedGoogle Scholar
- Bidanel JP, Milan D, Iannuccelli N, Amigues Y, Boscher MY, Bourgeois F, Caritez JC, Gruand J, Le RP, Lagant H: Detection of quantitative trait loci for growth and fatness in pigs. Genet Sel Evol. 2001, 33: 289-309. 10.1186/1297-9686-33-3-289.PubMed CentralView ArticlePubMedGoogle Scholar
- Li WB, Ren J, Zhu WC, Guo BL, Yang B, Liu LT, Ding NS, Ma JW, Li L, Huang LS: Mapping QTL for porcine muscle fibre traits in a White Duroc × Erhualian F(2) resource population. J Anim Breed Genet. 2009, 126: 468-474. 10.1111/j.1439-0388.2009.00805.x.View ArticlePubMedGoogle Scholar
- Ma J, Ren J, Guo Y, Duan Y, Ding N, Zhou L, Li L, Yan X, Yang K, Huang L: Genome-wide identification of quantitative trait loci for carcass composition and meat quality in a large-scale White Duroc × Chinese Erhualian resource population. Anim Genet. 2009, 40: 637-647. 10.1111/j.1365-2052.2009.01892.x.View ArticlePubMedGoogle Scholar
- Nii M, Hayashi T, Mikawa S, Tani F, Niki A, Mori N, Uchida Y, Fujishima-Kanaya N, Komatsu M, Awata T: Quantitative trait loci mapping for meat quality and muscle fiber traits in a Japanese wild boar × Large White intercross. J Anim Sci. 2005, 83: 308-315.PubMedGoogle Scholar
- de Koning DJ, Pong-Wong R, Varona L, Evans GJ, Giuffra E, Sanchez A, Plastow G, Noguera JL, Andersson L, Haley CS: Full pedigree quantitative trait locus analysis in commercial pigs using variance components. J Anim Sci. 2003, 81: 2155-2163.PubMedGoogle Scholar
- Edwards DB, Ernst CW, Raney NE, Doumit ME, Hoge MD, Bates RO: Quantitative trait locus mapping in an F2 Duroc × Pietrain resource population: II. Carcass and meat quality traits. J Anim Sci. 2008, 86: 254-266.View ArticlePubMedGoogle Scholar
- Malek M, Dekkers JC, Lee HK, Baas TJ, Prusa K, Huff-Lonergan E, Rothschild MF: A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. II. Meat and muscle composition. Mamm Genome. 2001, 12: 637-645. 10.1007/s003350020019.View ArticlePubMedGoogle Scholar
- Rohrer GA, Thallman RM, Shackelford S, Wheeler T, Koohmaraie M: A genome scan for loci affecting pork quality in a Duroc-Landrace F population. Anim Genet. 2006, 37: 17-27. 10.1111/j.1365-2052.2005.01368.x.View ArticlePubMedGoogle Scholar
- Sanchez MP, Iannuccelli N, Basso B, Bidanel JP, Billon Y, Gandemer G, Gilbert H, Larzul C, Legault C, Riquet J: Identification of QTL with effects on intramuscular fat content and fatty acid composition in a Duroc × Large White cross. BMC Genet. 2007, 8: 55-PubMed CentralView ArticlePubMedGoogle Scholar
- Thomsen H, Lee HK, Rothschild MF, Malek M, Dekkers JC: Characterization of quantitative trait loci for growth and meat quality in a cross between commercial breeds of swine. J Anim Sci. 2004, 82: 2213-2228.PubMedGoogle Scholar
- Tribout T, Iannuccelli N, Druet T, Gilbert H, Riquet J, Gueblez R, Mercat MJ, Bidanel JP, Milan D, Le RP: Detection of quantitative trait loci for reproduction and production traits in Large White and French Landrace pig populations. Genet Sel Evol. 2008, 40: 61-78.PubMed CentralPubMedGoogle Scholar
- Sanchez MP, Iannuccelli N, Basso B, Foury A, Billon Y, Gandemer G, Gilbert H, Mormede P, Bidanel JP, Larzul C: Microsatellite mapping of quantitative trait loci affecting meat quality, stress hormones and production traits in Duroc × Large White F2 pigs. animal. 2010, 1-8.Google Scholar
- Harmegnies N, Davin F, De SS, Buys N, Georges M, Coppieters W: Results of a whole-genome quantitative trait locus scan for growth, carcass composition and meat quality in a porcine four-way cross. Anim Genet. 2006, 37: 543-553. 10.1111/j.1365-2052.2006.01523.x.View ArticlePubMedGoogle Scholar
- Taniguchi M, Hayashi T, Nii M, Yamaguchi T, Fujishima-Kanaya N, Awata T, Mikawa S: Fine mapping of quantitative trait loci for meat color on Sus scrofa chromosome 6: analysis of the swine NUDT7 gene1. J Anim Sci. 2010, 88: 23-31. 10.2527/jas.2009-1814.View ArticlePubMedGoogle Scholar
- Taniguchi M, Hayashi T, Nii M, Yamaguchi T, Fujishima-Kanaya N, Awata T, Mikawa S: Overexpression of NUDT7, a candidate quantitative trait locus for pork color, downregulates heme biosynthesis in L6 myoblasts. Meat Sci. 2010, 86: 728-732. 10.1016/j.meatsci.2010.05.045.View ArticlePubMedGoogle Scholar
- Duan YY, Ma JW, Yuan F, Huang LB, Yang KX, Xie JP, Wu GZ, Huang LS: Genome-wide identification of quantitative trait loci for pork temperature, pH decline, and glycolytic potential in a large-scale White Duroc × Chinese Erhualian resource population. J Anim Sci. 2009, 87: 9-16.View ArticlePubMedGoogle Scholar
- Sanchez MP, Riquet J, Iannuccelli N, Gogue J, Billon Y, Demeure O, Caritez JC, Burgaud G, Feve K, Bonnet M: Effects of quantitative trait loci on chromosomes 1, 2, 4, and 7 on growth, carcass, and meat quality traits in backcross Meishan × Large White pigs. J Anim Sci. 2006, 84: 526-537.PubMedGoogle Scholar
- Ovilo C, Perez-Enciso M, Barragan C, Clop A, Rodriquez C, Oliver MA, Toro MA, Noruera JL: A QTL for intramuscular fat and backfat thickness is located on porcine chromosome 6. Mamm Genome. 2000, 11: 344-346. 10.1007/s003350010065.View ArticlePubMedGoogle Scholar
- Grindflek E, Szyda J, Liu Z, Lien S: Detection of quantitative trait loci for meat quality in a commercial slaughter pig cross. Mamm Genome. 2001, 12: 299-304. 10.1007/s003350010278.View ArticlePubMedGoogle Scholar
- Mohrmann M, Roehe R, Knap PW, Looft H, Plastow GS, Kalm E: Quantitative trait loci associated with AutoFOM grading characteristics, carcass cuts and chemical body composition during growth of Sus scrofa. Anim Genet. 2006, 37: 435-443. 10.1111/j.1365-2052.2006.01492.x.View ArticlePubMedGoogle Scholar
- Sato S, Oyamada Y, Atsuji K, Nade T, Sato S, Kobayashi E, Mitsuhashi T, Nirasawa K, Komatsuda A, Saito Y: Quantitative trait loci analysis for growth and carcass traits in a Meishan × Duroc F2 resource population. J Anim Sci. 2003, 81: 2938-2949.PubMedGoogle Scholar
- Gilbert H, LeRoy P, Milan D, Bidanel JP: Linked and pleiotropic QTLs influencing carcass composition traits detected on porcine chromosome 7. Genet Res. 2007, 89: 65-72. 10.1017/S0016672307008701.View ArticlePubMedGoogle Scholar
- Sato S, Hasebe H, Sato S, Asahi Y, Hayashi T, Kobayashi E, Sugimoto Y: High-resolution physical mapping and construction of a porcine contig spanning the intramuscular fat content QTL. Anim Genet. 2006, 37: 113-120. 10.1111/j.1365-2052.2005.01397.x.View ArticlePubMedGoogle Scholar
- Markljung E, Braunschweig MH, Karlskov-Mortensen P, Bruun CS, Sawera M, Cho IC, Hedebro-Velander I, Josell A, Lundstrom K, von SG: Genome-wide identification of quantitative trait loci in a cross between Hampshire and Landrace II: meat quality traits. BMC Genet. 2008, 9: 22-PubMed CentralView ArticlePubMedGoogle Scholar
- Cherel P, Glenisson J, Figwer P, Pires J, Damon M, Franck M, Le RP: Updated estimates of HAL n and RN- effects on pork quality: fresh and processed loin and ham. Meat Sci. 2010, 86: 949-954. 10.1016/j.meatsci.2010.07.022.View ArticlePubMedGoogle Scholar
- Barnes BR, Marklund S, Steiler TL, Walter M, Hjalm G, Amarger V, Mahlapuu M, Leng Y, Johansson C, Galuska D: The 5'-AMP-activated protein kinase gamma3 isoform has a key role in carbohydrate and lipid metabolism in glycolytic skeletal muscle. J Biol Chem. 2004, 279: 38441-38447. 10.1074/jbc.M405533200.View ArticlePubMedGoogle Scholar
- Lundström K, Enfalt AC, Tornberg E, Agerhem H: Sensory and technological meat quality in carriers and non-carriers of the RN- allele in Hampshire crosses and in purebred Yorkshire pigs. Meat Science. 2001, 48: 115-124.View ArticleGoogle Scholar
- Sellier P: Genetics of meat and carcass traits. The Genetics of the Pig. Edited by: Rothschild M, Ruvinsky A. 1998, CABI INTERNATIONAL, 436-510.Google Scholar
- Otsu K, Khanna VK, Archibald AL, MacLennan DH: Cosegregation of porcine malignant hyperthermia and a probable causal mutation in the skeletal muscle ryanodine receptor gene in backcross families. Genomics. 1991, 11: 744-750. 10.1016/0888-7543(91)90083-Q.View ArticlePubMedGoogle Scholar
- Lundström K, Karlsson A, Håkansson J, Hansson I, Johansson M, Andersson L, Andersson K: Production, carcass and meat quality traits of F2-crosses between European Wild Pigs and domestic pigs including halothane gene carriers. Animal Science. 1995, 61: 325-331. 10.1017/S1357729800013874.View ArticleGoogle Scholar
- Spach KM, Case LK, Noubade R, Petersen CB, McElvany B, Zalik N, Hickey WF, Blankenhorn EP, Teuscher C: Multiple linked quantitative trait loci within the Tmevd2/Eae3 interval control the severity of experimental allergic encephalomyelitis in DBA/2J mice. Genes Immun. 2010, 11: 649-659. 10.1038/gene.2010.40.PubMed CentralView ArticlePubMedGoogle Scholar
- Fawcett GL, Jarvis JP, Roseman CC, Wang B, Wolf JB, Cheverud JM: Fine-mapping of obesity-related quantitative trait loci in an F9/10 advanced intercross line. Obesity (Silver Spring). 2010, 18: 1383-1392. 10.1038/oby.2009.411.View ArticleGoogle Scholar
- Fridman E, Liu YS, Carmel-Goren L, Gur A, Shoresh M, Pleban T, Eshed Y, Zamir D: Two tightly linked QTLs modify tomato sugar content via different physiological pathways. Mol Genet Genomics. 2002, 266: 821-826. 10.1007/s00438-001-0599-4.View ArticlePubMedGoogle Scholar
- Sinha H, David L, Pascon RC, Clauder-Munster S, Krishnakumar S, Nguyen M, Shi G, Dean J, Davis RW, Oefner PJ: Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast. Genetics. 2008, 180: 1661-1670. 10.1534/genetics.108.092932.PubMed CentralView ArticlePubMedGoogle Scholar
- Larzul C, Le RP, Gueblez R, Talmant A, Gogue J, Sellier P: Effect of the halothane genotype (NN, Nn, nn) on growth, carcass and meat quality traits of pigs slaughtered at 95 kg or 125 kg live weight. J Anim Breed Genet. 1997, 114: 309-320. 10.1111/j.1439-0388.1997.tb00516.x.View ArticlePubMedGoogle Scholar
- Salmi B, Trefan L, Bloom-Hansen J, Bidanel JP, Doeschl-Wilson AB, Larzul C: Meta-analysis of the effect of the halothane gene on 6 variables of pig meat quality and on carcass leanness. J Anim Sci. 2010, 88: 2841-2855. 10.2527/jas.2009-2508.View ArticlePubMedGoogle Scholar
- Monin G, Sellier P: Pork of low technological quality with a normal rate of muscle pH fall in the immediate post-mortem period: The case of the Hampshire breed. Meat Science. 1985, 13: 49-63. 10.1016/S0309-1740(85)80004-8.View ArticlePubMedGoogle Scholar
- Miller SA, Dykes DD, Polesky HF: A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 1988, 16: 1215-10.1093/nar/16.3.1215.PubMed CentralView ArticlePubMedGoogle Scholar
- Rohrer GA, Alexander LJ, Hu Z, Smith TP, Keele JW, Beattie CW: A comprehensive map of the porcine genome. Genome Res. 1996, 6: 371-391. 10.1101/gr.6.5.371.View ArticlePubMedGoogle Scholar
- Kim KS, Larsen N, Short T, Plastow G, Rothschild MF: A missense variant of the porcine melanocortin-4 receptor (MC4R) gene is associated with fatness, growth, and feed intake traits. Mamm Genome. 2000, 11: 131-135. 10.1007/s003350010025.View ArticlePubMedGoogle Scholar
- Ciobanu D, Bastiaansen J, Malek M, Helm J, Woollard J, Plastow G, Rothschild M: Evidence for new alleles in the protein kinase adenosine monophosphate-activated gamma(3)-subunit gene associated with low glycogen content in pig skeletal muscle and improved meat quality. Genetics. 2001, 159: 1151-1162.PubMed CentralPubMedGoogle Scholar
- Heath SC: Markov chain Monte Carlo segregation and linkage analysis for oligogenic models. Am J Hum Genet. 1997, 61: 748-760. 10.1086/515506.PubMed CentralView ArticlePubMedGoogle Scholar
- Green P, Helms C, Weiffenbach B, Stephens K, Keith T, Bowden D, Smith D, Donis-Keller H: Construction of a linkage map of the human genome, and its application to mapping genetic diseases. Clin Chem. 1989, 35: B33-B37.PubMedGoogle Scholar
- Hawken RJ, Murtaugh J, Flickinger GH, Yerle M, Robic A, Milan D, Gellin J, Beattie CW, Schook LB, Alexander LJ: A first-generation porcine whole-genome radiation hybrid map. Mamm Genome. 1999, 10: 824-830. 10.1007/s003359901097.View ArticlePubMedGoogle Scholar
- Meyers SN, Rogatcheva MB, Larkin DM, Yerle M, Milan D, Hawken RJ, Schook LB, Beever JE: Piggy-BACing the human genome II. A high-resolution, physically anchored, comparative map of the porcine autosomes. Genomics. 2005, 86: 739-752. 10.1016/j.ygeno.2005.04.010.View ArticlePubMedGoogle Scholar
- Otsu K, Phillips MS, Khanna VK, de LS, MacLennan DH: Refinement of diagnostic assays for a probable causal mutation for porcine and human malignant hyperthermia. Genomics. 1992, 13: 835-837. 10.1016/0888-7543(92)90163-M.View ArticlePubMedGoogle Scholar
- Laville E, Sayd T, Terlouw C, Chambon C, Damon M, Larzul C, Leroy P, Glenisson J, Cherel P: Comparison of sarcoplasmic proteomes between two groups of pig muscles selected for shear force of cooked meat. J Agric Food Chem. 2007, 55: 5834-5841. 10.1021/jf070462x.View ArticlePubMedGoogle Scholar
- Sayd T, Morzel M, Chambon C, Franck M, Figwer P, Larzul C, Le RP, Monin G, Cherel P, Laville E: Proteome analysis of the sarcoplasmic fraction of pig semimembranosus muscle: implications on meat color development. J Agric Food Chem. 2006, 54: 2732-2737. 10.1021/jf052569v.View ArticlePubMedGoogle Scholar
- Lander ES, Botstein D: Mapping mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics. 1989, 121: 185-199.PubMed CentralPubMedGoogle Scholar
- George AW, Visscher PM, Haley CS: Mapping quantitative trait loci in complex pedigrees: a two-step variance component approach. Genetics. 2000, 156: 2081-2092.PubMed CentralPubMedGoogle Scholar
- Rowe SJ, Windsor D, Haley CS, Burt DW, Hocking PM, Griffin H, Vincent J, de Koning DJ: QTL analysis of body weight and conformation score in commercial broiler chickens using variance component and half-sib analyses. Anim Genet. 2006, 37: 269-272. 10.1111/j.1365-2052.2006.01424.x.View ArticlePubMedGoogle Scholar
- Gilmour AR, Gogel BJ, Cullis BR, Thompson R: ASReml User Guide Reference 2.0. 2006, VSN International LtdGoogle Scholar
- Xu S, Atchley WR: A random model approach to interval mapping of quantitative trait loci. Genetics. 1995, 141: 1189-1197.PubMed CentralPubMedGoogle Scholar
- Meuwissen TH, Goddard ME: Multipoint identity-by-descent prediction using dense markers to map quantitative trait loci and estimate effective population size. Genetics. 2007, 176: 2551-2560. 10.1534/genetics.107.070953.PubMed CentralView ArticlePubMedGoogle Scholar
- Hu ZL, Reecy JM: Animal QTLdb: beyond a repository. A public platform for QTL comparisons and integration with diverse types of structural genomic information. Mamm Genome. 2007, 18: 1-4. 10.1007/s00335-006-0105-8.View ArticlePubMedGoogle Scholar
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