Genetic divergence and the genetic architecture of complex traits in chromosome substitution strains of mice
© Spiezio et al.; licensee BioMed Central Ltd. 2012
Received: 30 November 2011
Accepted: 18 May 2012
Published: 18 May 2012
The genetic architecture of complex traits strongly influences the consequences of inherited mutations, genetic engineering, environmental and genetic perturbations, and natural and artificial selection. But because most studies are under-powered, the picture of complex traits is often incomplete. Chromosome substitution strains (CSSs) are a unique paradigm for these genome surveys because they enable statistically independent, powerful tests for the phenotypic effects of each chromosome on a uniform inbred genetic background. A previous CSS survey in mice and rats revealed many complex trait genes (QTLs), large phenotypic effects, extensive epistasis, as well as systems properties such as strongly directional phenotypic changes and genetically-determined limits on the range of phenotypic variation. However, the unusually close genetic relation between the CSS progenitor strains in that study raised questions about the impact of genetic divergence: would greater divergence between progenitor strains, with the corresponding changes in gene regulation and protein function, lead to significantly more distinctive phenotypic features, or alternatively would epistasis and systems constraints, which are pervasive in CSSs, limit the range of phenotypic variation regardless of the extent of DNA sequence variation?
We analyzed results for an extensive survey of traits in two new panels of CSSs where the donor strains were derived from inbred strains with more distant origins and discovered a strong similarity in genetic and systems properties among the three CSS panels, regardless of divergence time.
Our results argue that DNA sequence differences between host and donor strains did not substantially affect the architecture of complex traits, and suggest instead that strong epistasis buffered the phenotypic effects of genetic divergence, thereby constraining the range of phenotypic variation.
The phenotypic consequences of inherited mutations and genetic engineering as well as resilience to systems perturbations and response to natural and artificial selection depend heavily on the genetic architecture of complex traits . This architecture includes features such as the number of genes, the nature of DNA sequence differences, the effects of dominance, the extent of epistasis, and the range of pleiotropic actions . But in most cases, we have little genome-wide sense of these features because most studies do not have adequate statistical power. However, new genetic resources, innovations in genotyping, sequencing and phenotyping technologies, and novel analytical methods now enable rigorous studies. Adequately powered studies have recently been reported in yeast, Arabidopsis, cotton, Drosophila, chickens, beetles and other species, with discovery of many complex trait genes (also known as quantitative trait loci, or QTLs) and their interactions [see for example refs. [3–7], but mammalian studies remain especially challenging [8, 9].
Chromosome substitution strains (CSSs), also known as consomic strains, provide a special opportunity to characterize the architecture of complex traits in model organisms . CSSs are made by introgressing individual chromosomes from a donor strain onto a host strain background. The first complete CSS panel in mammals was derived by replacing each chromosome in the C57BL/6J (B6) inbred strain with the corresponding chromosome from the A/J strain . Additional mammalian CSS panels have subsequently been made for mice  and rats . Although their genetic constitution is highly unusual, CSSs have many unique attributes for gene discovery, functional studies, and systems analysis [14–16]. In particular, by controlling the phenotypic noise of background genetic variation, CSSs have considerable power to identify QTLs and to characterize other genetic features such as epistasis and systems properties such as phenotypic buffering (so-called ‘ceiling and floor’ effects) that are often lost in the background noise of segregating populations [17–23].
With the C57BL/6J-ChrA/J CSS panel, we studied the architecture of complex traits in laboratory mice, focusing on gene number, the nature of their interactions, and related systems properties . This study found at least 466 QTLs among 90 blood, bone and metabolic traits. The picture of genetic architecture was definitive and unexpected. On average, 8 of the 22 CSSs in the mouse panel differed significantly from the host strain for each multigenic trait, with an average phenotypic effect of 76% of the parental difference. Epistasis was detected for 98% of the 41 multigenic traits where the parental strains also differed significantly. The median cumulative phenotypic effect was 803% (range 164% to 1,397%), demonstrating striking non-additivity across these 41 traits. In addition, chromosome substitution led to phenotypic changes that were with few exceptions in the direction of the phenotypic state of the donor strain. Finally, the genetics of the parental strains defined physiological boundaries that largely constrained the range of phenotypic variation. Similar results were found with a rat CSS panel , suggesting that these results may be a general feature of inbred strains of mammals, and perhaps of segregating crosses and natural populations, rather than idiosyncrasies of particular traits, strains or species.
Although our study provided new insights about complex traits, many important questions remain, such as the extent to which the close genetic similarity between the progenitor strains affected the results, and whether substituted chromosomes from more distantly related inbred strains would result in more extreme phenotypes and distinct genetic architectures. With increased divergence time, DNA sequence differences accumulate, affecting transcription control and protein functions, and as a consequence phenotypic variation typically increases. But extensive epistasis could buffer sequence differences and constrain the range of phenotypic variation. In the extreme case, genetic background often buffers the phenotypic consequences of loss-of-function mutations [16–19].
Progenitors of mouse CSS panels differ substantially in divergence times. The B6 and A/J strains were derived in the early 1900s from closely related founders among Castle’s mice  and have since been inbred for hundreds of generations (jaxmice.jax.org). Although these two strains have many distinct phenotypes (http://www.jax.org/phenome), they are highly similar genetically and their genomes are substantially from the Mus musculus domesticus subspecies and many segments of these genomes are identical by descent from a common founder population [25–27]. By contrast, two new CSS panels were recently reported where chromosomes from more distantly related inbred strains were used as a source of donor chromosomes for substitution onto a B6 inbred genetic background [12, 28]. PWD/Ph was derived from mice that belong to the Mus musculus musculus subspecies and that were trapped in the Czech Republic in 1972 . MSM/Ms belongs to the Mus musculus molossinus subspecies and was established from wild mice trapped in Mishima Japan in 1978 . It is estimated that Mus musculus musculus and Mus musculus domesticus diverged from their common ancestor around 350,000 to 500,000 years ago whereas the divergence time between Mus musculus molossinus and Mus musculus domesticus is estimated roughly around 1 million years [30–33]. Our study design takes advantage of the introgression of chromosomes from genetically distant strains onto the same host strain background as the C57BL/6J-ChrA/J CSSs.
In this report, we tested whether genetic divergence between host and donor progenitors for CSSs affected the genetic architecture of complex traits. This test was based on comparing a panel of traits for these two new panels of CSSs with results for the original C57BL/6J-ChrA/J panel. The A/J strain diverged from the B6 strain hundreds of generations ago, whereas the progenitors of the PWD and MSM strains diverged from B6 millions of generations ago . As a result, gene regulation and protein functions should differ substantially in the more divergent PWD and MSM strains, both of which show a ~4-fold higher rate of DNA sequence differences than the A/J versus B6 comparison [25–27]. For both panels, we found many large-effect QTLs and pervasive epistasis, as well as strong systems properties that together corroborated results of the original study [14, 15]. More importantly, these genetic and phenotypic properties were remarkably similar among the three CSS panels, suggesting that genetic divergence did not have a strong impact on measures of phenotypic variation and that systems properties resulting from pervasive epistasis limited the range of phenotypic variation.
Strains and phenotypes
The panel of C57BL/6J-ChrPWD/Ph CSSs was developed by replacing each B6 chromosome with the corresponding chromosome from the PWD/Ph donor strain. Although the original PWD/Ph panel consisted of 28 strains, only 16 have data presently available in the Mouse Phenome Database (MPD, http://phenome.jax.org/db/q?rtn=projects/projdet&reqprojid=219) . In addition, among these 16 strains, two chromosomes, 11 and X, are each represented with three and two congenic strains because of breeding problems in mice with an intact substituted chromosome. Although absence of a chromosome can result from husbandry issues or from epistasis that compromises fertility or viability, the necessity for two congenic strains rather than a single substituted chromosome is strong evidence for epistasis. In total, 76 traits (38 for males and 38 for females) were downloaded from MPD, including measurements of blood counts and chemistry as well as traits related to bone biology.
The panel of C57BL/6J-ChrMSM/Ms CSSs was made by replacing each C57BL/6J chromosome with the corresponding chromosome of the donor MSM/Ms strain . This panel consists of 28 strains, with chromosomes 2, 6, 7, 12, 13, and X each represented with two congenic strains that either carried the centromeric (C) or telomeric (T) segments of these chromosomes. CSS-10MSM/Ms is absent from the panel because of breeding problems. As with the PWD panel, the need for congenic strains is strong evidence for epistasis.
In total, 71 traits were analyzed. Detailed assay methods and results are published for organ weights, blood chemistry and bone biology traits  and available at http://molossinus.lab.nig.ac.jp/phenotype/index.html. Briefly, carcasses were weighed at sacrifice (10 weeks of age) to measure lean body weight. Heart, liver, spleen, kidney and testis were dissected, weighed, and indicated as a percentage of total lean weight. To measure fat pad weight, dorsal and inguinal deposits of subcutaneous white adipose tissues, epididymal, gonadal and mesenteric deposits for visceral white adipose tissues, and inter-scapular and sub-scapular brown adipose deposits were dissected, weighed individually, and indicated as a percentage of total fasting body weight. All animal experiments were approved by the Animal Care and Use Committee of National Institute of Genetics, Mishima, Japan.
For all three CSS panels, care was taken to include a broad range of traits and assays so that phenotyping results were not biased inordinately by idiosyncrasies of particular classes of physiological systems and biological traits. For logistical and historical reasons, similar but not identical assays were surveyed in the three CSS panels [11, 12].
Genetic differences between strains
The patterns of sequence divergence among these strains were obtained from [25–27], (see also http://phenome.jax.org/pub-cgi/phenome/mpdcgi?rtn=snps/Perlegen2_matrix) and were augmented with new sequencing data for the MSM/Ms strain (TT and TS, in preparation). The pairwise polymorphism frequencies were 14% for C57BL/6J and A/J, 57% for C57BL/6J and PWD/Ph, and 54% for C57BL/6J and MSM/Ms. These results are similar to results for more complete sequence analysis of these and other closely related strains [26, 27].
To be consistent with published studies , we focused on traits where at least 3 CSSs differed significantly from the host strain, so that the focus was on multigenic rather than monogenic or digenic control of phenotypic variation. Because most trait measurements were conducted on 8 to 12 mice, with some traits measured in only 4 mice, especially in the C57BL/6J-ChrPWD/Ph CSS panel, we did not use the 3 interquantile range (IQR) to filter outliers (cf. re. 15). Unpaired t-tests were used to determine whether a CSS differed significantly from the host strain after Bonferroni correction for testing multiple strains but not for testing multiple traits.
Because differences in sample sizes and statistical power varied among traits and panels, we examined the frequency distribution of phenotypic effects to test whether significant biases were found among the three CSS panels, but the distributions were similar suggesting that sample size variation did not substantially affect results (Additional file 1: Figure).
Two published methods were used to estimate the magnitude of phenotypic effects . Effect sizes were measured first as a function of the phenotypic difference between the CSS donor and host strains (NormUnit), and second as a proportion of the total phenotypic variance, which was defined by the range of the average phenotypic value among the parental strains and CSSs for each trait (Hi_Low). These methods yielded highly similar results (not shown, but see ref. 17). These alternative measures were needed because the genetic composition of each CSS preclude more conventional estimates of variance and effect size [10, 11, 14, 16].
The directionality of QTL effects was classified into two categories: “moving away from” and “moving towards” trait values for the donor strain (cf. 15). A variable r, which was defined as (Thost – TCSS)/(Thost – Tdonor), was used to demonstrate directionality. When r > 0, the CSS shifted the phenotype “towards” the donor strain, whereas when r < 0, the CSS shifted the phenotype “away from” the donor strain. For r > 1, where the CSS shifted the trait value towards the donor strain, results were also tested to determine whether more extreme trait values than the donor strain were found.
To test for epistasis for each trait, the cumulative signed phenotypic effect was estimated and the standard error propagation method was used to estimate the standard error of measurement (SEM) . We concluded that epistasis dominated phenotypic effects if the absolute value of the cumulative effect minus its SEM exceeded 100%. We note that this somewhat unusual test for non-additivity simply extends across the genome a commonly used method for testing epistasis at pairs of loci [28, 29, 32–35].
Results and discussion
C57BL/6J-ChrPWD CSSs – Mus musculus musculus
QTL summary for the three CSS panels
No. of traits
Multigenic traits (n)
Digenic traits (n)
Monogenic traits (n)
No. of multigenic QTLs
QTL number and average phenotypic effect for multigenic traits in the three CSS panels
No. of multigenic traits
Ave. no. QTLs per trait (min, max)
Ave. phenotypic effect (%)(min, max)
75.9 (24.4, 148.4)
153.0 (29.0, 408.6)
47.4 (16.6, 148.4)
Percentage of traits showing non-additive effects (epistasis) and the cumulative phenotypic effects for CSSs with significant effects, non-significant effects, and all CSSs combined
No. of traits
Cumulative phenotypic effect
Combined (all) effects
% traits showing epistasis
Med % (min,max)
% traits showing epistasis
Med % (min,max)
% traits showing epistasis
Med % (min,max)
605.5 (41.0, 1205.8)
168.3 (0.8, 643.4)
790.3 (6.5, 1397.2)
404.0 (16.8, 3176.9)
250.1 (51.4, 1182.5)
751.8 (53.2, 3630.8)
247.3 (90.7, 1073.7)
166.4 (0.3, 523.0)
371.7 (156.2, 1589.4)
Directionality for multigenic traits where the parental strains differed significantly
“away from” donor
“more extreme“ than donor
C57BL/6J-ChrMSM CSSs – Mus musculus molossinus
Among the 71 traits that were surveyed for the 28 CSSs in the C57BL/6J-ChrMSM/Ms panel, 39 were multigenic, and a total of 283 QTLs differed significantly from the host strain (Table 1). We then focused on the 22 traits where the parental MSM/Ms and C57BL/6J strains also differed significantly (Table 2). For these traits, an average of 7.2 CSSs differed significantly from C57BL/6J, at least 163 QTLs were discovered, and the average phenotypic effect of these CSSs was 47.4% of the parental difference.
We next examined the cumulative effects for these traits across the C57BL/6J-ChrMSM/Ms CSS panel (Table 3, Figure 1B). Among the 22 traits, 91% demonstrated strong epistasis for CSSs with significant effects, with a median cumulative effect of 247.3% (range 117.6% to 1,073.7%), for CSSs with non-significant effects, 50% traits showed epistasis, with a median cumulative effect of 166.4%, and for all CSSs, 100% (22) of the traits showed non-additive effects, with a median cumulative effect of 371.7%.
Lastly, we focused on directionality of phenotypic effects (Table 4, Figure 2B). Among the 163 CSSs with statistically significant effects, 88.1% shifted the phenotype ‘towards’ the MSM/Ms donor strain and only 11.9% shifted ‘away from’ the donor strain. Even for those non-significant CSSs, 70% (311 of 447) shifted ‘towards’ and 30% (136 of 447) shifted ‘away from’ the donor strain, again indicating a strong bias towards the donor strain. None of the CSSs had trait values that were more extreme than the donor strain. The parental strains defined both boundaries for 17 (77%) of the 22 traits, and one boundary for 5 traits.
Effects of genetic divergence on phenotypic variation
Features of genetic architecture and systems properties in the three CSS panels
Percentage of traits that are multigenic
Ave. no. of QTLs per trait
Ave. phenotypic effect (%)
Percentage of traits showing epistasis
Median cum. effect
“away” from donor
Interestingly, several lines of evidence suggest that CSSs that were derived from more divergent strain combinations were not consistently more extreme than those from more recently derived progenitors (Table 5). First, the average number of CSSs that differed from the host strain across the panel of traits was similar for A/J and MSM, and both were somewhat greater than the number for PWD. Second, the percentage of traits showing epistasis was similar among the three CSS panels (range 86% to 100%). Third, the average phenotypic effect was significantly greater for A/J versus B6 than for MSM versus B6, but significantly less than PWD versus B6, i.e. PWD > A/J > MSM. Fourth, the median cumulative phenotypic effect was similar for A/J versus B6 and PWD versus B6, and both were roughly twice the effect for MSM versus B6, i.e. A/J = PWD > MSM. Finally, the phenotypic shift “away from donor” was significantly stronger for PWD versus B6 than for the two other CSS versus B6 comparisons, i.e. PWD > A/J = MSM. Thus we found no consistent evidence that genetic divergence systematically affected genetic architecture or systems properties.
The incomplete nature of the PWD and MSM CSS panels has implications for several measures of genetic architecture and systems properties. In particular, the number of QTLs as well as their cumulative phenotypic effect may be under-estimated because the missing CSSs may carry QTLs affecting traits of interest, whereas estimates of average phenotypic effect size as well as trends in systems properties such as directionality or boundaries of phenotypic effects should not be affected. If the four missing strains from the PWD panel and the one strain missing from the MSM panel were similar to those that were studied, we estimate 6.7 QTLs (=5.5 x 22/18) for PWD and 8.1 QTLs (= 7.2 x 22/21) for MSM respectively for CSSs that have at least one QTL with a significant phenotypic effect. Thus complete panels would probably have provided even more consistent results across the three CSS panels. Similarly, pairs of congenic strains rather than a complete substituted chromosome should not significantly affect the results because they maintain the CSS paradigm of single large genome segments isolated on a defined genetic background.
Finally, we note that while ‘ceiling-floor’ effects might constrain the range of phenotypic variation for individual traits, these effects have negligible impact on measures of phenotypic variation across the CSS panels. Indeed, the ‘percentage of traits’ was the only measure that was ‘saturated’ with all three panels approaching 100%; the four other measures could have yielded more extreme results, i.e. additional ‘CSSs with significant effects’ could have been discovered, and the ‘average effect size’, the ‘median cumulative phenotypic effect’, and the ‘phenotypic shift’ could each have been greater.
Although theoretical aspects of the relation between functional differences and genetic divergence have not been studied extensively, the reasonable expectation is that increasing genetic divergence should lead to more dramatic phenotypic and systems differences. The present study not only corroborated results of the original CSS survey [14, 36], but also showed that differences in regulatory elements and protein functions related to genetic divergence between progenitor strains did not lead to more extreme phenotypic variation. We analyzed CSS panels that were derived from two genetically distant Mus musculus subspecies, Mus musculus musculus (PWD/Ph; and Mus musculus molossinus (MSM/Ms; ), respectively. Genotyping surveys as well as complete genome sequencing reveals a ~4-fold higher rate of DNA sequence variants between the more divergent strains (PWD and MSM versus B6) as compared to the more closely related strains (A/J versus B6) [25–27]. Surveys of a broad variety of traits in all three panels revealed large phenotypic effects and pervasive epistasis for the majority of traits. Overall, the phenotypic features for CSSs derived from these more genetically divergent MSM and PWD inbred strains were remarkably similar to those derived from the A/J inbred strain (Table 5).
To account for phenotypic features that are largely independent of the extent of genetic divergence, we propose that organisms are composed of non-random combinations of allelic variants that provide not only the necessary conditions for viability, fertility and other essential aspects of organismal biology, but resilience to environmental and genetic perturbations. Under these conditions, genetic divergence is less important than favorable combinations of genetic variants. Studies of spontaneous, induced and engineered mutations, modifier genes, and genetic background effects support the hypothesis that epistasis buffers organisms from the adverse phenotypic effects of deleterious mutations and naturally-occurring genetic variants [17–19].
Issues that emerge from this work include developing a theory that accounts for the ways that functional differences scale with genetic divergence and obtaining experimental evidence for the molecular and systems properties that buffer organisms from environmental and genetic perturbations.
In this study, we tested whether genetic divergence leads to more distinct phenotypes, or alternatively whether epistasis constrains patterns of phenotypic variation. The expectation was that various measures of phenotypic differences and several systems properties would be quite distinct in that the new CSS panels have 4-fold difference in DNA sequence divergence versus the original panel. We compared patterns of phenotypic variation among three panels of chromosome substitution strains (CSSs) of mice, and the remarkably similar results across the panels suggest that epistasis, which is a pervasive feature in these strains, constrained the patterns of phenotypic variation. These results contribute to the growing body of evidence that biological systems tend to be composed predominately of epistatic networks of genes action rather than collections of genetic variants with independent and additive effects.
This work was supported by NIH NCRR grant RR12305 to JHN and by KAKENHI (Grant-in-Aid for Scientific Research) on Priority Areas “Comparative Genomics & Comprehensive Genomics" from the Ministry of Education, Culture, Sports, Science and Technology of Japan to TT and TS.
- Hensen TF: The evolution of genetic architecture. Annu Rev Ecol Evol Syst. 2006, 37 (1): 123-157. 10.1146/annurev.ecolsys.37.091305.110224.View Article
- Flint J, Mackay TFC: Genetic architecture of quantitative traits in mice, flies, and humans. Genome Res. 2009, 19: 723-733. 10.1101/gr.086660.108.PubMed CentralView ArticlePubMed
- Wright D, Rubin CJ, Martinez Barrio A, Schutz K, Kerje S, Brandstrom H, Kindmark A, Jensen P, Andersson L: The genetic architecture of domestication in the chicken: effects of pleiotropy and linkage. Mol Ecol. 2010, 19: 5140-5156. 10.1111/j.1365-294X.2010.04882.x.View ArticlePubMed
- Cubillos FA, Billi E, Zorgo E, Parts L, Fargier P, Omholt S, Blomberg A, Warringer J, Louis EJ, Liti G: Assessing the complex architecture of polygenic traits in diverged yeast populations. Mol Ecol. 2011, 20: 1401-1413. 10.1111/j.1365-294X.2011.05005.x.View ArticlePubMed
- Saha S, Wu J, Jenkins J, McCarty J, Hayes R, Stelly D: Delineation of interspecific epistasis on fiber quality traits in Gossypium hirsutum by ADAA analysis of intermated G. barbadense chromosome substitution lines. TAG Theor Appl Genet. 2011, 122: 1351-1361. 10.1007/s00122-011-1536-5.View Article
- Fox CW, Czesak ME, Wallin WG: Complex genetic architecture of population differences in adult lifespan of a beetle: nonadditive inheritance, gender differences, body size and a large maternal effect. J Evol Biol. 2004, 17: 1007-1017. 10.1111/j.1420-9101.2004.00752.x.View ArticlePubMed
- Ehrenreich IM, Torabi N, Jia Y, Kent J, Martis S, Shapiro JA, Gresham D, Caudy AA, Kruglyak L: Dissection of genetically complex traits with extremely large pools of yeast segregants. Nature. 2010, 464: 1039-1042. 10.1038/nature08923.PubMed CentralView ArticlePubMed
- Schadt EE: Molecular networks as sensors and drivers of common human diseases. Nature. 2009, 461: 218-223. 10.1038/nature08454.View ArticlePubMed
- Lander ES: Initial impact of the sequencing of the human genome. Nature. 2011, 470: 187-197. 10.1038/nature09792.View ArticlePubMed
- Nadeau JH, Singer JB, Matin A, Lander ES: Analysing complex genetic traits with chromosome substitution strains. Nat Genet. 2000, 24: 221-225. 10.1038/73427.View ArticlePubMed
- Singer JB, Hill AE, Burrage LC, Olszens KR, Song J, Justice M, O'Brien WE, Conti DV, Witte JS, Lander ES, et al: Genetic dissection of complex traits with chromosome substitution strains of mice. Science. 2004, 304: 445-448. 10.1126/science.1093139.View ArticlePubMed
- Takada T, Mita A, Maeno A, Sakai T, Shitara H, Kikkawa Y, Moriwaki K, Yonekawa H, Shiroishi T: Mouse inter-subspecific consomic strains for genetic dissection of quantitative complex traits. Genome Res. 2008, 18: 500-508. 10.1101/gr.7175308.PubMed CentralView ArticlePubMed
- Malek RL, Wang H-y, Kwitek AE, Greene AS, Bhagabati N, Borchardt G, Cahill L, Currier T, Frank B, Fu X, et al: Physiogenomic resources for rat models of heart, lung and blood disorders. Nat Genet. 2006, 2: 234-239. 10.1038/nchembio0506-234.View Article
- Shao H, Burrage LC, Sinasac DS, Hill AE, Ernest SR, O'Brien W, Courtland H-W, Jepsen KJ, Kirby A, Kulbokas EJ, et al: Genetic architecture of complex traits: Large phenotypic effects and pervasive epistasis. Proc Natl Acad Sci. 2008, 105: 19910-19914. 10.1073/pnas.0810388105.PubMed CentralView ArticlePubMed
- Yazbek SN, Buchner DA, Geisinger JM, Burrage LC, Spiezio SH, Zentner GE, Hsieh C-W, Scacheri PC, Croniger CM, Nadeau JH: Deep congenic analysis identifies many strong, context-dependent QTLs, one of which, Slc35b4, regulates obesity and glucose homeostasis. Genome Res. 2011, 21: 1065-1073. 10.1101/gr.120741.111.PubMed CentralView ArticlePubMed
- Belknap J: Chromosome substitution strains: some quantitative considerations for genome scans and fine mapping. Mamm Genome. 2003, 14: 723-732. 10.1007/s00335-003-2264-1.View ArticlePubMed
- Nadeau JH: Modifier genes in mice and humans. Nat Rev Genet. 2001, 2: 165-174.View ArticlePubMed
- Dowell RD, Ryan O, Jansen A, Cheung D, Agarwala S, Danford T, Bernstein DA, Rolfe PA, Heisler LE, Chin B, et al: Genotype to phenotype: a complex problem. Science. 2010, 328: 469-10.1126/science.1189015.PubMed CentralView ArticlePubMed
- Boone C, Bussey H, Andrews BJ: Exploring genetic interactions and networks with yeast. Nat Rev Genet. 2007, 8: 437-449. 10.1038/nrg2085.View ArticlePubMed
- Bergman A, Siegal ML: Evolutionary capacitance as a general feature of complex gene networks. Nature. 2003, 424: 549-552. 10.1038/nature01765.View ArticlePubMed
- Hermisson J, Wagner GP: The population genetic theory of hidden variation and genetic robustness. Genetics. 2004, 168: 2271-2284. 10.1534/genetics.104.029173.PubMed CentralView ArticlePubMed
- Jasnos L, Korona R: Epistatic buffering of fitness loss in yeast double deletion strains. Nat Genet. 2007, 39: 550-554. 10.1038/ng1986.View ArticlePubMed
- Musso G, Costanzo M, Huangfu M, Smith AM, Paw J, San Luis B-J, Boone C, Giaever G, Nislow C, Emili A, et al: The extensive and condition-dependent nature of epistasis among whole-genome duplicates in yeast. Genome Res. 2008, 18: 1092-1099. 10.1101/gr.076174.108.PubMed CentralView ArticlePubMed
- Beck JA, Lloyd S, Hafezparast M, Lennon-Pierce M, Eppig JT, Festing MF, Fisher EM: Geneologies of mouse inbred strains. Nat Genet. 2000, 24: 23-25. 10.1038/71641.View ArticlePubMed
- Frazer KA, Eskin E, Kang HM, Bogue MA, Hinds DA, Beilharz EJ, Gupta RV, Montgomery J, Morenzoni MM, Nilsen GB, et al: A sequence-based variation map of 8.27 million SNPs in inbred mouse strains. Nature. 2007, 448: 1050-1053. 10.1038/nature06067.View ArticlePubMed
- Keane TM, Goodstadt L, Danecek P, White MA, Wong K, Yalcin B, Heger A, Agam A, Slater G, Goodson M, et al: Mouse genomic variation and its effect on phenotypes and gene regulation. Nature. 2011, 477: 289-294. 10.1038/nature10413.PubMed CentralView ArticlePubMed
- Yang H, Wang JR, Didion JP, Buus RJ, Bell TA, Welsh CE, Bonhomme F, Yu AH-T, Nachman MW, Pialek J, et al: Subspecific origin and haplotype diversity in the laboratory mouse. Nat Genet. 2011, 43: 648-655. 10.1038/ng.847.PubMed CentralView ArticlePubMed
- Gregorová S, Divina P, Storchova R, Trachtulec Z, Fotopulosova V, Svenson KL, Donahue LR, Paigen B, Forejt J: Mouse consomic strains: Exploiting genetic divergence between Mus m. musculus and Mus m. domesticus subspecies. Genome Res. 2008, 18: 509-515. 10.1101/gr.7160508.PubMed CentralView ArticlePubMed
- Gregorova S, Forejt J: PWD/Ph and PWK/Ph inbred mouse strains of Mus m. musculus subspecies–a valuable resource of phenotypic variations and genomic polymorphisms. Folia Biol. 2000, 46: 31-41.
- Moriwaki K, Miyashita N, Mita A, Gotoh H, Tsuchiya K, Kato H, Mekada K, Noro C, Oota S, Yoshiki A, et al: Unique intred strain MSM/Ms established from the Japanese wild mouse. Exp Anim. 2009, 58: 123-134. 10.1538/expanim.58.123.View ArticlePubMed
- Yonekawa H, Moriwaki K, Gotoh O, Watanabe J, Hayashi J-i, Miyasita N, Petras Ml, Tagashira Y: Relationship between laboratory mice and the subspecies mus musculus domesticus based on restriction endonuclease cleavage patterns of mitochondrial DNA. Japanese J Genet. 1980, 55: 289-296. 10.1266/jjg.55.289.View Article
- She JX, Bonhomme F, Boursot P, Thaler L, Catzeflis F: Molecular phylogenies in the genus Mus: Comparative analysis of electrophoretic, scnDNA hybridization, and mtDNA RFLP data. Biol J Linn Soc. 1990, 41: 83-103. 10.1111/j.1095-8312.1990.tb00823.x.View Article
- Geraldes A, Basset P, Smith KL, Nachman MW: Higher differentiation among subspecies of the house mouse (Mus musculus) in genomic regions with low recombination. Mol Ecol. 2011, 20: 4722-4736. 10.1111/j.1365-294X.2011.05285.x.PubMed CentralView ArticlePubMed
- Svenson K, Forejt J, Donahue L, Paigen B: Multi-system survey of mouse physiology in C57BL/6J-Chr#PWD/Ph/ForeJ chromosome substitution panel. 2006, http://phenome.jax.org/db/q?rtn=projects/details&sym=Svenson2,
- Peters LL, Shavit JA, Lambert AJ, Tsaih S-W, Li Q, Su Z, Leduc MS, Paigen B, Churchill GA, Ginsburg D, et al: Sequence variation at multiple loci influences red cell hemoglobin concentration. Blood. 2010, 116: e139-e149. 10.1182/blood-2010-05-283879.PubMed CentralView ArticlePubMed
- Boell L, Gregorova S, Forejt J, Tautz D: A comparative assessment of mandible shape in a consomic strain panel of the house mouse (Mus musculus) - implications for epistasis and evolvability of quantitative traits. BMC Evol Biol. 2011, 11: 309-10.1186/1471-2148-11-309.PubMed CentralView ArticlePubMed
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.