Comparative analysis of sex chromosomes in Leporinusspecies (Teleostei, Characiformes) using chromosome painting
© Parise-Maltempi et al.; licensee BioMed Central Ltd. 2013
Received: 15 March 2013
Accepted: 1 July 2013
Published: 3 July 2013
The Leporinus genus, belonging to the Anostomidae family, is an interesting model for studies of sex chromosome evolution in fish, particularly because of the presence of heteromorphic sex chromosomes only in some species of the genus. In this study we used W chromosome-derived probes in a series of cross species chromosome painting experiments to try to understand events of sex chromosome evolution in this family.
W chromosome painting probes from Leporinus elongatus, L. macrocephalus and L. obtusidens were hybridized to each others chromosomes. The results showed signals along their W chromosomes and the use of L. elongatus W probe against L. macrocephalus and L. obtusidens also showed signals over the Z chromosome. No signals were observed when the later aforementioned probe was used in hybridization procedures against other four Anostomidae species without sex chromosomes.
Our results demonstrate a common origin of sex chromosomes in L. elongatus, L. macrocephalus and L. obtusidens but suggest that the L. elongatus chromosome system is at a different evolutionary stage. The absence of signals in the species without differentiated sex chromosomes does not exclude the possibility of cryptic sex chromosomes, but they must contain other Leporinus W sequences than those described here.
KeywordsFISH Zoo-FISH Microdissection Sex chromosome evolution
The origin and evolution of sex chromosomes have interested evolutionary biologists for a long time. Although sex chromosomes evolve from an autosomal pair , over time they become different both in gene content and structure . While sex chromosomes in most mammals are ancient, sex chromosomes in some fish, insects and dioecious plants are evolutionarily young [2, 3].
Among vertebrates, fish present an enormous diversity of sex determination mechanisms, contrasting with the much more stable systems found in mammals and birds. The majority of teleost fish are gonochoristic, meaning that they exist as males and females, and the most gonochoristic species have genetic sex determination. Beside simultaneous and sequential hermaphroditism, gonochorism in fish is controlled by many forms of sex determination involving genetic and/or environmental factors, although temperature-dependent sex determination might be rarer than previously thought [4, 5]. Despite a large number of fish species described, only a minor fraction has been thoroughly investigated and their sex determination mechanism unequivocally clarified .
Within the fish species with genetic sex determination many variations have been found, ranging from male or female heterogamety to polygenic sex determination. Multiple sex chromosomes, or autosomal modifiers that enhance or antagonize the sex-determining genes on the gonosomes, are quite frequent [7, 8]. This, together with the fact that even between closely related fish species the sex determination mechanisms can be different [6, 9–13], demonstrates the high evolutionary plasticity of this fundamental process .
Sex chromosomes in the genus Leporinus (Teleostei, Anostomidae) present some features that make this group very interesting for the study of sex determination mechanisms and sex chromosome structure, composition and evolution. In general, the sex chromosomes of the Leporinus genus have morphological similarities. An exception is observed in the Leporinus sp2 species, where the W chromosomes are not differentiated from autosomes. The Leporinus group consists of around 40 species and eight of them show heteromorphic sex chromosomes and the presence of conspicuous large blocks of heterochromatin on sex chromosomes is the characteristic feature of the Leporinus karyotypes. Among this eight species seven (L. conirostris, L. elongatus, L. aff. elongatus, L. macrocephalus, L. obtusidens, L. reinhardti, L. trifasciatus) possess enlarged W chromosomes in comparison to the Z, due the increase of heterochromatin, while one, Leporinus sp2, presents a karyotype with the W chromosome equal in size to its homologous Z and the autosomes. The hypothesis of the accumulation of repetitive elements as the major pathway in the differentiation of sex chromosomes in Anostomidae is widely accepted [14, 15]. In fact, it has been proposed that repetitive elements are different among some species  and that they were probably responsible for the recent origin of a multiple Z1Z1Z2Z2/Z1W1Z2W2 sex chromosome system in L. elongatus. However whether the increase of heterochromatic segments by accumulation of repetitive DNA is the cause or consequence of sex chromosome origin remains, as yet, undiscovered and details of sex chromosome evolution within the genus are unknown.
To study sex chromosome evolution in Leporinus and related Anostomidae species we have obtained whole W chromosome specific probes of three Leporinus species by manual microdissection and used the paints for cross species hybridisation. Our results provide some new views of Anostomidae chromosome evolution and add new evidence on the origin of sex chromosomes in Leporinus.
Hybridisation using Leporinus elongatus Wprobe (WLe)
FISH experiments with WLe probes in L. friderici, L. striatus, L. lacustris, Schizodon borelii and S. isognathus individuals did not produce any result (even under low stringency wash conditions) (data not shown).
Hybridisation using Leporinus macrocephalus Wprobe (WLm)
Hybridisation using Leporinus obtusidens Wprobe (WLo)
Discussion and conclusion
The W chromosome of Leporinus species is easily recognised, being the largest chromosome of the karyotype due to the accumulation of large heterochromatic blocks, which facilitate the identification of the chromosome during microdissection procedures. While the sex chromosomes of the Leporinus species in general are highly differentiated, the other chromosomes show the typical metacentric- submetacentric pattern . The morphologic similarity among sex chromosomes found in the Leporinus species led to the hypothesis of a common origin, in which an initial accumulation of heterochromatic segments was the first step in the differentiation of sex chromosomes . An exception was found in Leporinus sp2, where the size of the W chromosome was similar to other elements of the karyotype . These authors proposed that the sex chromosomal system in Leporinus sp2 originated independently from the ZW system previously described for other Leporinus species.
Our hybridisation experiments show that WLe hybridizes solely to the W chromosome in L. elongatus, while in L. macrocephalus and L. obtusidens the Z chromosomes were also partly labelled. The WLm and WLo probes reveal a similar result in cross species experiments, the most intense signals being those located at heterochromatic regions of the q arms. This pattern is possibly due the distribution of repetitive elements.
The use of repetitive elements to map the dynamics of sex chromosomes in Anostomidae has been successfully employed. The repetitive element LeSpeI was described as a participant of the sex chromosome differentiation process in L. elongatus. This sequence was also mapped in L. macrocephalus and L. obtusidens, revealing homologies and highlighting their importance in sex chromosome differentiation . However, the attempt to find a relationship between LeSpeI and a putative cryptic sex differentiation process in Leporinus friderici, L. striatus, L. lacustris, Schizodon borelii and S. isognathus by mapping this sequence in these species without sex chromosomes, failed because no positive hybridisation signals were observed .
The Leporinus genus has been divided hypothetically into two different groups according to cytogenetic and molecular records [18, 22]. Our data reinforce the idea of the subdivision of Leporinus genus in at least two lineages: one comprising species with differentiated sex chromosomes and the other without sex chromosome differentiation. The W specific probes retrieved from L. elongatus, L. macrocephalus and L. obtusidens systematically cross hybridised with other W chromosomes, showing the similarity of these sequences. Based on this fact, it is possible to hypothesize that the sex chromosomes of L. elongatus, L. macrocephalus and L. obtusidens had a common origin. According to our results, the hypothesis of cryptic sex chromosomes sharing Leporinus W sequences in the other anostomids (Leporinus friderici, L. striatus, L. lacustris, Schizodon borelii and S. isognathus) can be discarded, consistent with previous studies on repetitive sequences. Furthermore, those sex chromosomes could have originated from the same autosomes, but in a different process of differentiation.
A fact to note in our results is that the probes of the W chromosome from L. elongatus paint the Z chromosome of L. macrocephalus males and females and the Z chromosome of L. obtusidens male but do not paint the L. elongatus Z chromosome present in males and females. This reinforces the hypothesis proposed by Parise-maltempi et al.  about the presence of a new system of multiple sex chromosomes in L. elongatus. The sex chromosomes of L. elongatus are probably in a different stage of differentiation from those of the other Leporinus species in which repetitive sequences shared between the Z and W chromosomes are not shared in L. elongatus chromosome Z.
Cross-species chromosome painting is a powerful tool to delineate syntenic chromosome regions among closely and distantly related species and to reconstruct chromosomal phylogeny [23–25]. In fish specifically, studies have brought new insights into the evolution of sex chromosome systems, and showed that in many cases, closely related groups have taken quite different evolutionary paths [9, 10, 26–29]. As in Leporinus species analysed in this report, some other fish groups also share a common origin of sex chromosomes [12, 30–32].
Our data indicate that in spite of the often reported high plasticity of sex determination mechanisms in fish, in the Anostomidae family, and especially in some species of the Leporinus genus, the sex systems seem to be conserved and derived from a common ancestral pair. Probably the sex chromosomes are actually different, but the different W chromosomes could have been invaded with the same repetitive sequences. The results shown here add new data to studies using the Leporinus genus as a model, reinforcing the hypothesis that the Leporinus ZW sex chromosomes may be found only in closely related species, and remain undetected in the other species.
Cell culture and metaphase cell preparation
Leporinus elongatus chromosomes were obtained from cultured cells. Fibroblast cells collected from fin tissue of L. elongatus were cultured in DMEM medium supplemented with 10% FCS and 50% Amniomax C-100 (Invitrogen) serum at 37°C.
The cells were harvested after colcemid treatment (0.05 ug/mL) for 2 hours, suspended in hypotonic solution (0.075M KCl) and then incubated at 37°C for 20 min. Cells were pelleted and resuspended three times in fresh ice cold fixative (3:1 methanol:acetic acid), then kept at −20°C until use.
The chromosomes of other Anostomidae species (L. friderici, L. striatus, L. lacustris, Schizodon borelii and S. isognathus) were obtained by direct cytological preparations from kidney samples according to the methods given by Foresti et al. .
Chromosome microdissection and DNA amplification
Chromosome suspensions were dropped onto moist, 1% SDS- cleaned coverslips. The coverslips were washed in 1x PBS solution for 1 minute and incubated in a trypsin solution (35 mL 1x PBS and 5 mL trypsin) for 30 seconds, washed again in 1x PBS and stained with Giemsa 1% in PBS.
The microdissection was performed using an Eppendorf TransferMan NK2 (Eppendorf) coupled to a Zeiss Axiovert 40 CFL microscope, with glass needles made with a Nikon puller and sterilized by UV radiation. About 8 chromosomes were separately placed in 9uL DNAse-free ultrapure water and were then amplified using the GenomePlex Single Cell Whole Genome Amplification Kit (WGA4-Sigma).
Fluorescent in situhybridisation (FISH)
The probes were labelled using the GenomePlex (WGA3 Reamplification KIT-Sigma) following the manufacturer’s protocol, except changing the kit dNTP mix for a ½ T dNTP mix and adding a biotin 16-dUTP in the reaction.
Slides were dehydrated through an ethanol series; aged for 1h at 60°C, denatured in 70% formamide/0.6x saline-sodium citrate (SSC) at 65°C for 2 minutes and dehydrated again. 3 μL of biotinylated probe combined with L. elongatus Cot 1 (2 μg) was precipitated in ethanol and resuspended in 13 μL of hybridisation buffer (40% deionized formamide (v/v), 10% dextran sulfate, 2x SSC, 0.05 M phosphate buffer, pH 7.3). This mixture was denatured for 10 min at 65°C, pre-annealed at 37°C for 1 hour and applied to each slide. Hybridisation was carried out at 37°C for 12 hours for the same species and over three nights for cross species experiments. It was used Cot1 once that Fish genomes possess a high amount of repetitive elements and FISH with chromosome specific probes gives a large amount of background signal if no suppression of repetitive elements is used. Post-hybridisation washes were performed in 40% formamide/1.8x SSC twice for 5 min each, followed by 2x SSC twice for 5 min each and 4x SSC with 0.05% Tween-20 (4xT) once for 4 min at 42°C for the same species and 45°C for cross species. Probe detection was carried out using 200 μL of diluted (1:500) Cy3-streptavidin antibody (Amersham) per slide at 37°C for 30 min. After detection, slides were washed in 4xT three times for 3 min each at 42°C and mounted in Vectashield mounting medium with 4′,6- diamidino-2-phenylindole (DAPI; Vector Laboratories).
Images were captured and processed using the CytoVision Genus system (Applied Imaging, USA) and a Cohu CCD camera mounted on an Olympus BX-60 microscope.
The authors are grateful to Fundação de Amparo a Pesquisa do Estado de São Paulo – FAPESP and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – CAPES for the financial support. They also thank Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis (IBAMA) for the licensing of collection to ELS.
- Ohno S: Sex chromosomes and sex-linked genes. 1967, Berlin: SpringerView ArticleGoogle Scholar
- Charlesworth D, Charlesworth B, Marais G: Steps in the evolution of heteromorphic sex chromosomes. Heredity. 2005, 95: 118-128. 10.1038/sj.hdy.6800697.View ArticlePubMedGoogle Scholar
- Vyskot B, Hobza R: Gender in plants: sex chromosomes are emerging from the fog. Trends Genet. 2004, 20: 432-438. 10.1016/j.tig.2004.06.006.View ArticlePubMedGoogle Scholar
- Ospina-Álvarez N, Piferrer F: Temperature-dependent sex determination in fish revisited: prevalence, a single sex ratio response pattern, and possible effects of climate change. PLoS ONE. 2008, 3 (7): 2837-10.1371/journal.pone.0002837.View ArticleGoogle Scholar
- Schultheis C, Böhne A, Schartl M, Volff J, Galiana-Arnoux D: Sex determination diversity and sex chromosome evolution in poeciliid fish. Sex Dev. 2009, 3: 68-77. 10.1159/000223072.View ArticlePubMedGoogle Scholar
- Devlin RH, Nagahama Y: Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Aquaculture. 2002, 208: 191-364. 10.1016/S0044-8486(02)00057-1.View ArticleGoogle Scholar
- Herpin A, Schartl M: Molecular mechanisms of sex determination and evolution of the Y chromosome: Insights from the medaka fish (Oryzias latipes). Mol Cell Endocrin. 2009, 306: 1-2. 10.1016/j.mce.2009.03.018.View ArticleGoogle Scholar
- Hattori RS, Murai Y, Oura M, Masuda S, Majhi SK, Sakamoto T, Fernandino JI, Somoza GM, Yokota M, Strussmann CA: A Y-linked anti-Mullerian hormone duplication takes over a critical role in sex determination. Proc Natl Acad Sci US. 2012, 109 (8): 2955-9. 10.1073/pnas.1018392109.View ArticleGoogle Scholar
- Cioffi MB, Sánchez A, Marchal JA, Kosyakova N, Liehr T, Trifonov V, Bertollo LAC: Cross-species chromosome painting tracks the independent origin of multiple sex chromosomes in two cofamiliar Erythrinidae fishes. BMC Evolutionary Biology. 2011, 11: 1-10. 10.1186/1471-2148-11-1.View ArticleGoogle Scholar
- Takehana Y, Hamaguchi S, Sakaizumi M: Different origins of ZZ/ZW sex chromosomes in closely related medaka fishes, Oryzias javanicus and O. hubbsi. Chrom Res. 2008, 16: 801-811. 10.1007/s10577-008-1227-5.View ArticlePubMedGoogle Scholar
- Tanaka K, Takehana Y, Naruse K, Hamaguchi S, Sakaizumi M: Evidence for different origins of sex chromosomes in closely related Oryzias fishes: substitution of the master sex-determining gene. Genetics. 2007, 177: 2075-2081. 10.1534/genetics.107.075598.PubMed CentralView ArticlePubMedGoogle Scholar
- Ota K, Kobayashi T, Ueno K, Gojobori T: Evolution of heteromorphic sex chromosomes in the order Aulopiformes. Gene. 2000, 23: 25-30.View ArticleGoogle Scholar
- Volff JN, Schartl M: Variability of genetic sex determination in Poeciliid fishes. Genetica. 2001, 111: 101-110. 10.1023/A:1013795415808.View ArticlePubMedGoogle Scholar
- Nakayama I, Foresti F, Tewari R, Schartl M, Chourrout D: Sex chromosome polymorphism and heterogametic males revealed by two cloned DNA probes in the ZW/ZZ fish Leporinus elongatus. Chromosoma. 1994, 103: 31-39. 10.1007/BF00364723.View ArticlePubMedGoogle Scholar
- Parise-Maltempi PP, Martins C, Oliveira C, Foresti F: Identification of a new repetitive element in the sex chromosomes of Leporinus elongatus (Teleostei: Characiformes: Anostomidae): new insights into the sex chromosomes of Leporinus. Cytogenet Gen Res. 2007, 116: 218-223. 10.1159/000098190.View ArticleGoogle Scholar
- Silva EL, Busso AF, Parise-Maltempi PP: Characterization and genome organization of a repetitive element associated with the nucleolar organizer region in Leporinus elongatus (Anostomidae: Characiformes). Cytogenet Gen Res. 2013, 139: 22-28. 10.1159/000342957.View ArticleGoogle Scholar
- Galetti PM, Foresti F, Bertollo LAC, Moreira Filho O: Karyotypic similarity in three genera (Leporinus, Leporellus, Schizodon) of the family Anostomidae (Pisces, Teleostei). Brazil J Genet. 1981, 4: 11-15.Google Scholar
- Galetti PM, Lima NRW, Venere PC: A monophyletic ZW sex chromosome system in Leporinus (Anostomidae, Characiformes). Cytologia. 1995, 60: 375-382. 10.1508/cytologia.60.375.View ArticleGoogle Scholar
- Venere PC, Ferreira IA, Martins C, Galetti PM: A novel ZZ/ZW sex chromosome system for the genus Leporinus. (Pisces, Anostomidae, Characiformes). Genetica. 2004, 121: 75-80.View ArticlePubMedGoogle Scholar
- Marreta ME, Faldoni FLC, Parise-Maltempi PP: Cytogenetic mapping of the W chromosome in the genus Leporinus (Teleostei, Anostomidae) using a highly repetitive DNA sequence. J Fish Biol. 2012, 80: 630-637. 10.1111/j.1095-8649.2011.03199.x.View ArticlePubMedGoogle Scholar
- Silva EL, Borba RS, Parise-Maltempi PP: Chromosome mapping of repetitive sequences in Anostomidae species: implications for genomic and sex chromosome evolution. Molecular Cytogenetics. 2012, 5: 45-52. 10.1186/1755-8166-5-45.PubMed CentralView ArticlePubMedGoogle Scholar
- Ferreira IA, Oliveira C, Venere P, Galetti PM, Martins C: 5S rDNA variation and its phylogenetic inference In the genus Leporinus (Characiformes: Anostomidae). Genetica. 2007, 129: 253-257. 10.1007/s10709-006-0005-6.View ArticlePubMedGoogle Scholar
- Nanda I, Benisch P, Fetting D, Haaf T, Schmid M: Synteny conservation of chicken macrochromosomes 1–10 in different avian lineages revealed by cross-species chromosome painting. Cytogenet Gen Res. 2011, 132: 165-181. 10.1159/000322358.View ArticleGoogle Scholar
- Ferguson-Smith MA, Trifonov V: Mammalian karyotype evolution. Nat Rev Gene. 2007, 8 (12): 950-62. 10.1038/nrg2199.View ArticleGoogle Scholar
- Graphodatsky AS, Trifonov VA, Stanyon R: The genome diversity and karyotype evolution of mammals. Mol Cytogenet. 2011, 4: 22-10.1186/1755-8166-4-22.PubMed CentralView ArticlePubMedGoogle Scholar
- Phillips RB, Konkol NR, Reed KM, Stein D: Chromosome painting supports lack of homology among sex chromosomes in Oncorhynchus, Salmo and Salvelinus (Salmonidae). Genetica. 2001, 111: 119-123. 10.1023/A:1013743431738.View ArticleGoogle Scholar
- Takehana Y, Naruse K, Hamaguchi S, Sakaizumi M: Evolution of ZZ/ZW and XX/XY sex-determination systems in the closely related medaka species, Oryzias hubbsi and O. dancena. Chromosoma. 2007, 116: 463-470. 10.1007/s00412-007-0110-z.View ArticlePubMedGoogle Scholar
- Takehana Y, Demiyah D, Naruse K, Hamaguchi S, Sakaizumi M: Evolution of different Y chromosomes in two medaka species, Oryzias dancena and O. latipes. Genetics. 2007, 175: 1335-1340.PubMed CentralView ArticlePubMedGoogle Scholar
- Henning F, Moysés CB, Calcagnotto D, Meyer A, Almeida-Toledo LF: Independent fusions and recent origins of sex chromosomes in the evolution and diversification of glass knife fishes (Eigenmannia). Heredity. 2011, 106: 391-400. 10.1038/hdy.2010.82.PubMed CentralView ArticlePubMedGoogle Scholar
- Diniz D, Laudicina A, Cioffi MB, Bertollo LAC: Microdissection and whole chromosome painting: Improving sex chromosome analysis in Triportheus (Teleostei, Characiformes). Cytogenet Gen Res. 2008, 122: 163-168. 10.1159/000163094.View ArticleGoogle Scholar
- Vicari MR, Artoni RF, Moreira-Filho O, Bertollo LAC: Diversification of a ZZ/ ZW sex chromosome system in Characidium fish (Crenuchidae, Characiformes). Genetica. 2008, 134: 311-317. 10.1007/s10709-007-9238-2.View ArticlePubMedGoogle Scholar
- Pansonato-Alves JCP, Vicari MR, Oliveira C, Foresti F: Chromosomal diversification in populations of Characidium cf. gomesi (Teleostei, Crenuchidae). J Fish Biol. 2011, 78: 183-194. 10.1111/j.1095-8649.2010.02847.x.View ArticlePubMedGoogle Scholar
- Foresti F, Oliveira C, Almeida-Toledo LF: A method for chromosome preparations from large specimens of fishes using in vitro short treatment with colchicine. Experientia. 1993, 49: 810-813. 10.1007/BF01923555.View ArticleGoogle Scholar
- Rens W, O'Brien PC, Yang F, Graves JA, Ferguson-Smith MA: Karyotype relationships between four distantly related marsupials revealed by reciprocal chromosome painting. Chromosome Res. 1999, 7: 461-74. 10.1023/A:1009249813617.View ArticlePubMedGoogle Scholar
- Rens W, Moderegger K, Skelton H, Clarke O, Trifonov V, Ferguson-Smith MA: A procedure for image enhancement in chromosome painting. Chromosome Res. 2006, 14: 497-503. 10.1007/s10577-006-1056-3.View ArticlePubMedGoogle Scholar
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