Mapping QTL conferring resistance in maize to gray leaf spot disease caused by Cercospora zeina
© Berger et al.; licensee BioMed Central Ltd. 2014
Received: 27 January 2014
Accepted: 15 May 2014
Published: 22 May 2014
Gray leaf spot (GLS) is a globally important foliar disease of maize. Cercospora zeina, one of the two fungal species that cause the disease, is prevalent in southern Africa, China, Brazil and the eastern corn belt of the USA. Identification of QTL for GLS resistance in subtropical germplasm is important to support breeding programmes in developing countries where C. zeina limits production of this staple food crop.
A maize RIL population (F7:S6) from a cross between CML444 and SC Malawi was field-tested under GLS disease pressure at five field sites over three seasons in KwaZulu-Natal, South Africa. Thirty QTL identified from eleven field trials (environments) were consolidated to seven QTL for GLS resistance based on their expression in at least two environments and location in the same core maize bins. Four GLS resistance alleles were derived from the more resistant parent CML444 (bin 1.10, 4.08, 9.04/9.05, 10.06/10.07), whereas the remainder were from SC Malawi (bin 6.06/6.07, 7.02/7.03, 9.06). QTLs in bin 4.08 and bin 6.06/6.07 were also detected as joint QTLs, each explained more than 11% of the phenotypic variation, and were identified in four and seven environments, respectively. Common markers were used to allocate GLS QTL from eleven previous studies to bins on the IBM2005 map, and GLS QTL “hotspots” were noted. Bin 4.08 and 7.02/7.03 GLS QTL from this study overlapped with hotspots, whereas the bin 6.06/6.07 and bin 9.06 QTLs appeared to be unique. QTL for flowering time (bin 1.07, 4.09) in this population did not correspond to QTL for GLS resistance.
QTL mapping of a RIL population from the subtropical maize parents CML444 and SC Malawi identified seven QTL for resistance to gray leaf spot disease caused by C. zeina. These QTL together with QTL from eleven studies were allocated to bins on the IBM2005 map to provide a basis for comparison. Hotspots of GLS QTL were identified on chromosomes one, two, four, five and seven, with QTL in the current study overlapping with two of these. Two QTL from this study did not overlap with previously reported QTL.
KeywordsGray leaf spot Grey leaf spot GLS Cercospora QTL Maize Corn
Gray leaf spot (GLS) is a foliar disease of maize that was highlighted as a threat to maize production in the USA in the 1980s , reported in South Africa in the 1990s , and currently has a worldwide distribution in maize production areas, including South America  and China . One of the main reasons for emergence of the disease in commercial settings is the increasing use of conservation tillage, which allows fungal inoculum to build up on crop residues . Adjacent or subsequent maize crops are then readily infected. Considering the environmental benefits of soil conservation, the two main control measures employed against GLS are the use of fungicides and deployment of maize hybrids with resistance to the disease [6, 7].
GLS disease is characterized by the formation of rectangular lesions on maize leaves, which reduce the photosynthetic potential and ultimately yield of the crop . GLS disease symptoms develop from the lower leaves upwards on a maize plant, reaching greatest intensity after flowering. Assessment of GLS disease on maize therefore often takes into account the progression of GLS lesions on a plant as well as the number and size of lesions [6, 8].
Two species of Cercospora, which belong to the Dothidiomycetes family of fungi characterized by many foliar plant pathogens, are the causal agents of GLS on maize. Cercospora zeae-maydis and Cercospora zeina were first categorized as two types of C. zeae-maydis, however they have now been classified into separate species [10, 11]. GLS lesions formed by each species appear to be identical, although growth in vitro and disease development is slower in C. zeina.
Differences in geographical distribution of the two species have been noted. C. zeae-maydis is the predominant pathogen in the USA, however C. zeina is found in the Eastern corn belt  and there are some regions where the ranges overlap, such as North Carolina . GLS is found on maize throughout sub-Saharan Africa, and C. zeina appears to be the causal agent in Africa, with isolates from Zambia, Zimbabwe, Kenya, Rwanda, Uganda and South Africa being classified into the C. zeina group when subjected to molecular analysis [10, 13, 14].
The identification and characterization of maize germplasm with resistance to GLS has been an ongoing goal of maize researchers. Published work indicates that maize resistance to GLS is quantitative in nature, with additive gene action being commonly reported [15–17]. No cases of single gene qualitative resistance to GLS have been reported, and isolates of the pathogen have not been classified into races based on host specificity. However, since the molecular basis of resistance to GLS has not been characterized yet, it still remains possible that quantitative resistance could be effected by so-called weak resistance (R) genes that recognize pathogen effectors in a gene-for-gene manner . A mechanism for this could be tolerance mediated through an R gene, as was recently shown in Arabidopsis pathosystems [19, 20].
Quantitative trait loci (QTL) for resistance to GLS have been reported from several studies using maize populations developed from bi-parental crosses of inbred lines, where one parent showed greater GLS resistance compared to the other. Initial studies in the USA prior to 2008 employed F2, F2:3 or backcross populations to identify QTL, which limited the number of environments (field sites and seasons) that each population could be tested in [21–24]. Population sizes were between 100–200 and relatively low numbers of molecular markers such as RFLP were used. However, QTL were identified on all ten chromosomes of maize, although they were defined to regions of up to 30 cM (centiMorgans).
Subsequent studies employed populations of recombinant inbred lines (RIL), such as the intermated B73 X Mo17 (IBM) population, which allowed repeated GLS disease testing in multiple environments coupled with high density molecular marker maps [25–27]. QTL for GLS resistance were located to regions down to 3 cM . Association mapping in a panel of 253 diverse inbred maize lines led to the identification of SNP polymorphisms in a glutathione S-transferase gene that were correlated with resistance to GLS .
Breeding programmes outside the USA have also led to the identification of QTL for GLS resistance. For example a South African inbred line V0613Y, first thought to carry a single major gene for resistance , was shown in parallel field trials at Cedara, KwaZulu-Natal, South Africa and Ohio, USA, to carry two QTL for GLS resistance . The QTL on chromosome four was validated in progeny of a cross with a different susceptible parent . In a separate study, two proprietary lines developed in southern Africa were used to detect different QTL for GLS resistance (on chromosome one and five) when a backcross population was tested at Hillcrest, a nearby site in KwaZulu-Natal, South Africa .
QTL for GLS disease resistance have been reported from field testing in Brazil of two different proprietary maize populations [3, 31]. QTL mapping for GLS resistance has also been carried out in China . A GLS resistance source from a breeding programme in Thailand was used. QTL on chromosomes five and eight were identified in repeated environments, and the QTL on chromosome eight was fine mapped to a region that corresponds to 1.5 Mb on the B73 genome . The field trials were conducted in Yunnan province of China, where C. zeina and not C. zeae-maydis was isolated from all 25 locations sampled .
QTL for resistance to gray leaf spot disease have not been reported to be specific to either C. zeae-maydis or C. zeina, however it is useful to test for QTL in environments where one or other of the species is present. In South Africa, GLS is most prevalent in the subtropical environment of KwaZulu-Natal, and we recently showed that only C. zeina was isolated from multiple sites in this region . We wished to identify sources of resistance to GLS caused by C. zeina in germplasm that had been developed under subtropical conditions in Africa, which could be taken up relatively quickly in local breeding programmes or deployed in hybrid combinations . RIL populations have the advantage of being “immortal” so replicated trials can be planted in multiple sites and seasons, which mitigates the problem that GLS QTL identification can be variable from season to season due to differences in environmental conditions that affect disease severity [21, 22].
The CML444 X SC Malawi RIL population was chosen for this study and planted at multiple sites in KwaZulu-Natal over a period of three years, resulting in the identification of seven QTL for GLS resistance. Two of these QTL corresponded to “hotspots” for the trait reported in previous studies on chromosome four and seven. In contrast, some QTL identified in this study have not been reported before, including one QTL on chromosome six that was identified in seven environments. This study represents one of the most comprehensive analyses of QTL for GLS due to the many field trials employed, and has a further advantage in defining QTL in response to C.zeina, since only this species is currently known to cause GLS in KwaZulu-Natal, South Africa.
Germplasm and field trials
A recombinant inbred line population (RIL, F7:S6) derived from a cross between subtropical white dent inbred lines CML444 and SC Malawi was used . A total of 145 RILs, that produced sufficient seed and were phenotypically homogenous among plants of a RIL, were planted at five locations over three summer rainfall seasons (2008, 2009 and 2010) in KwaZulu-Natal, South Africa. Field trials were situated at Baynesfield Estate, Cedara Agricultural College, Hildesheim Farm, Redgates Farm and Ukulinga Research Farm of the University of KwaZulu-Natal. These field trial sites are within a 100 km radius from the city of Pietermaritzburg. All field sites were exposed to natural infection by C. zeina, and in addition, V5-V7 stage plants were inoculated in the whorls with dried and powdered material from the previous season’s GLS diseased maize plants. GLS disease severity data was recorded for all three seasons at Redgates Farm, two out of the three seasons at Cedara Agricultural College, Baynesfield Estate and Ukulinga Research Farm, and a single season at Hildesheim Farm. An “environment” was defined as a field trial with a particular planting date at a particular location.
For each trial, the RILs were planted in a randomized block with two or three replicates. Each replicate of a RIL was a row of 10 plants. GLS disease severity was scored on a per row basis using a 1–9 scale, where 1 and 9 represent no GLS disease and complete GLS susceptibility, respectively . GLS disease scores were taken twice each season before and after anthesis, and a GLS disease score for each replicate row was obtained by averaging the scores from the time points.
Days to anthesis (DTA) was measured as days from planting to anthesis (pollen release) in 50% of plants in a row at Redgates Farm in the 2010 season. This field trial was distinct from those used for collection of GLS severity data since this represented a control plot sprayed with a fungicide regime and therefore no GLS symptoms were present (data not shown).
Statistical analysis of field trial data
GLS disease scores were analyzed using the analysis of variance (ANOVA) function of the car package in R statistical software [34, 35] to estimate the variance components attributable to environment (field trials), replicates within an environment, genotype and genotype × environment interactions. The PROC GLM procedure of SAS 9.3 (SAS Institute, Cary, NC) was used to calculate least square means of the GLS disease scores for each field trial using a mixed model approach considering replication as random effects and genotypes as fixed effects. These least square mean values were used to perform QTL mapping of the GLS disease phenotype for each field trial. In addition, “joint” GLS QTL for all environments (trials/seasons) were determined by (i) computing the z-score of the least square means of GLS disease scores for each RIL per environment; and (ii) using the mean z-score per RIL across all environments as input data for QTL mapping. Correlations between GLS disease phenotypes in different field trials were calculated using GraphPad Prism 5.04 (GraphPad Software Inc.).
Genetic map enrichment
The genetic map of the CML444 X SC Malawi population which was previously constructed with 79 RFLP and 81 SSR markers,  was scrutinized to identify regions where there were gaps of approximately 20 cM or more. SSR markers in these regions were selected from the Maize Genetics and Genomics database (http://www.maizegdb.org/). DNA was extracted from a pool of three plants per RIL for 145 RILs, and genotyped by polyacrylamide or agarose gel electrophoresis with the following SSR markers: bnlg1811, bnlg615, umc1111, phi073, bnlg1449, bnlg1108, umc1720, bnlg105, dupssr10, umc1155, umc1572, bnlg2191, umc1413, umc1424, umc1562, umc1170, bnlg1375, umc1137, umc1337. Genetic map construction was carried out using the genetic map data from Messmer et al.  plus the additional SSR marker genotypes using MapManager QTX software . As the mapping population consisted of 145 RILs, confidence in markers which were closer than 5 cM to another marker was low and therefore those markers were removed to reduce possible distortion of the map. The final genetic map, named QMap 2.0, was displayed using MapChart .
QTL for GLS disease severity as well as DTA in the CML444 X SC Malawi RIL population were identified for each field trial based on the genetic map QMap 2.0 and applying the Composite Interval Mapping (CIM) utility in Windows QTL Cartographer 2.5_011  using the standard model 6 with a window size of 10 cM and a 1 cM walk speed. Both forward and backward regression analysis was performed. The statistical significance LOD (logarithm of odds) threshold used to declare the presence of QTLs was obtained from 1000 permutations at a genome-wide significance level of 5% for each field trial [39, 40]. The 1- and 2-LOD support intervals were used to define each QTL region. Epistatic interactions between QTL were assessed by using the Multiple Interval Mapping (MIM) utility in Windows QTL Cartographer as previously described .
The maize core bin positions of QTL for GLS from literature were retrieved to compare with consensus QTL identified in the current study. Most publications designate GLS QTL into maize core bins [3, 4, 7, 25–27]. A maize genome bin has been defined as one of the 100 designated chromosomal segments between two core RFLP markers (http://www.maizegdb.org/cgi-bin/bin_viewer.cgi). The positions of these bin markers have been placed onto high density maize maps, such as the IBM2 2005 neighbours frame map (3287 well-ordered markers), as well as the B73 genome sequence which enables most studied QTLs to be placed into maize core bins based on common markers . The maize bin positions of GLS QTL from five studies prior to 2008 [21–24, 30] were taken from the analysis by Balint-Kurti et al. . The study of Juliatti et al.  could not be included in the comparison as there was insufficient information to place the proprietary SSR markers on the IBM2 2005 map.
Gray leaf spot disease assessment of maize RIL population
Analysis of variance of gray leaf spot (GLS) disease scores from a population consisting of 145 RILs from a CML444 X SC Malawi cross scored over eleven environments in KwaZulu-Natal, South Africa
Replications within environment
Genotype x environment
Genetic map construction
Identification of QTL for GLS resistance
QTL for gray leaf spot (GLS) resistance and days to anthesis (DTA) identified in the CML444 X SC Malawi RIL population $
264.7 - 281.0
264.3 - 285.7
270.9 - 285.6
267.5 - 289.7
29.6 - 46.2
25.3 - 50.1
201.8 - 219.6
207.9 - 219.6
0.0 - 8.2
0.0 - 13.3
121.9 - 133.8
116.5 - 136.6
122.6 - 132.9
120.9 - 136.0
122.9 - 136.1
120.4 - 140.5
127.9 - 139.3
127.9 - 142.3
141.0 - 151.7
135.3 - 154.7
142.7 - 160.5
139.2 - 164.3
142.3 - 161.4
139.0 - 162.3
147.3 - 166.1
143.7 - 174.0
147.5 - 162.1
143.1 - 169.6
150.7 - 162.0
147.7 - 168.5
148.3 - 163.9
143.2 - 174.4
10.0 - 28.7
9.0 - 30.7
20.4 - 29.7
20.1 - 30.8
22.1 - 36.5
20.4 - 40.5
34.4 - 41.1
33.0 - 43.0
30.8 - 42.8
30.8 - 44.6
72.2 - 86.3
67.2 - 87.4
83.0 - 97.3
75.6 - 97.6
92.9 - 101.3
77.8 - 105.5
115.0 - 122.8
110.7 - 124.5
119.8 - 123.6
119.8 - 124.7
120.3 - 123.6
120.0 - 124.4
23.4 - 40.5
16.6 - 45.5
114.0 - 127.9
110.9 - 129.8
113.4 - 129.2
108.7 - 129.9
122.3 - 134.4
120.4 - 138.0
148.8 - 161.4
146.2 - 167.2
189.7 - 202.0
187.5 - 217.2
141.6 - 149.8
138.0 - 153.1
The thirty QTL identified in eleven environments were consolidated into seven QTL that were detected in two or more environments and were placed in the same maize core bins based on common markers with the IBM2 2005 map (QTL in bin 1.10, 4.08, 6.06/6.07, 7.02/7.03, 9.04/9.05, 9.06, 10.06/10.07) plus six remaining QTL that were identified in one environment each (Table 2).
Comparison of QTL with previously identified QTL for GLS resistance
The CML444 X SC Malawi QTL identified in this study were compared with GLS resistance QTL identified in eleven previous studies based on the maize core bin positions of the QTL (Table 2) (Additional file 1).
Several of the GLS resistance QTL identified in the CML444 X SC Malawi population cluster with QTL found in previous studies (Additional file 1). The QTL on chromosome 1 (bin 1.10) overlaps with a QTL reported by Zhang et al. . The QTL with the allele associated with GLS resistance derived from CML444 in bin 4.08 was identified in four environments as well as the joint GLS QTL (Table 2, Additional file 1). Both inbred line VP31 and its progenitor VO163Y have a QTL in this bin [7, 24], which also overlap with QTL reported by Bubeck et al.  and Saghai Maroof et al. . The GLS QTL with the SC Malawi allele associated with resistance in bin 7.02/7.03 overlaps with QTL from four studies [3, 21, 23, 26] (Additional file 1). QTL with the resistance alleles from CML444 in bins 9.05 and 10.06 overlap with QTL from B73 and B73rhm, respectively [21, 25].
There were also several QTL for GLS resistance from the CML444 X SC Malawi population that have QTL that do not overlap with QTL reported in previous studies, notably (i) the bin 6.06/6.07 QTL with the SC Malawi allele associated with resistance that accounted for 9.3- 21.8% of the phenotypic variation in seven environments and as a joint GLS QTL; and (ii) the bin 9.06 QTL with the SC Malawi allele associated with resistance identified in three environments (Table 2, Additional file 1). Single environment GLS QTL not previously reported were identified in bin 3.02/3.03, bin 3.09, bin 4.01, bin 7.04, and bin 10.2 (Table 2).
QTL mapping of the CML444 X SC Malawi population in eleven environments in KwaZulu-Natal, South Africa, over three years revealed seven QTL for GLS disease resistance that were observed in at least two environments (GLS QTL in bin 1.10, bin 4.08, bin 6.06/6.07, bin 7.02/7.03, bin 9.04/9.05, bin 9.06, bin 10.06/10.07; Table 2). Detection of QTL for GLS resistance has been reported to be variable from site to site and season to season . For example, Saghai Maroof et al.  identified three QTL that were consistent in F2 and F3 generations and seasons, but another two QTL that were not. The use of a RIL population in our study allowed testing in multiple environments, which gave confidence in the detected QTL. Furthermore, the QTL were identified using an improved genetic map compared to the map reported by , since we included 19 additional gap-filling SSR markers (Figure 2).
Prior to our study, there were two reports of QTL for GLS resistance that were identified under field conditions in South Africa. Lehmensiek et al.  used a bi-parental backcross population from a proprietary source, and identified GLS resistance QTL on chromosome one (bin 1.05/1.06) and chromosome five (bin 5.03-5.06). In another study, QTL for GLS resistance in the inbred line V0613Y were mapped to chromosome four (bin 4.06/4.08) and chromosome two (bin 2.09) . The source of GLS resistance in this inbred line was proposed to be either a terminal-ear maize type or teosinte germplasm developed in a breeding programme in South Africa . These studies were carried out at field sites in KwaZulu-Natal that are within a 100 km radius of Pietermaritzburg and likely to have similar GLS disease pressure, although the diversity and identity of the Cercospora species was not known at the time. In the current study, we identified QTL in a different population derived from subtropical germplasm. SC Malawi was developed in Zimbabwe in the 1960s, whereas CML444 was developed by CIMMYT breeding programmes in Mexico and Zimbabwe . Consensus QTL from our study did not overlap with those reported by Lehmensiek et al. , whereas we did detect a QTL that coincided with the QTL on chromosome four from V0613Y (Additional file 1). This may reflect the uptake of antecedents of V0613Y in breeding programmes in southern Africa.
Some of the GLS resistance QTL identified from the CML444 X SC Malawi population in our study corresponded to clusters or “hotspots” of GLS resistance QTL reported in other populations (Additional file 1). Despite the limitations of comparing QTL positions between different studies which used different genetic maps, population sizes, and statistical tests to define QTL, it has proven useful to compare QTL based on a common framework of maize core bin regions [25, 42]. Previous identification of hotspots of GLS QTL were carried out prior to 2007 and included six populations [28, 43]. Visual inspection of the data of Wisser et al.  for QTL reported in at least three of the six populations revealed GLS QTL hotspots in bin 1.05/1.06, bin 2.05/2.06, bin 4.08, and bins 5.03 and 5.05 . These correspond relatively well with “consensus QTLs” defined by Shi et al.  who also projected QTL from the same studies onto the IBM2005 map, and noted seven “consensus QTLs” (bin 1.06, bin 2.06, bin 3.04, bin 4.06 (two), 4.08, 5.03 and 8.06).
Our study was able to extend the analyses of Wisser et al.  and Shi et al. , since there have been seven subsequent studies with different germplasm sources including our own (Additional file 1). We defined QTL hotspots as bins with QTL in four or more of thirteen populations, namely bin 1.05/1.06, bin 2.03/2.04, bin 4.08, bin 5.03/5.04 and bin 7.02/7.03 (Additional file 1). Our analysis confirms the previous demarcation of GLS QTL hotspots, and adds the hotspot on chromosome seven. The CML444 X SC Malawi population did not show QTL overlapping the bin 1.05/1.06 or bin 5.03/5.04 QTL hotspots, but did overlap with bin 4.08 and bin 7.02/7.03 hotspots.
Hotspot analysis by comparing QTLs from different studies has value in identifying common genomic regions across a germplasm collection with a significant effect on a trait of interest. For example, a hotspot may correspond to a common GLS resistance allele or may be a region that has multiple alleles/genes that can confer GLS resistance. Candidates for the latter are R genes, which are often found in clusters . Wisser et al.  pointed out R genes in GLS resistance hotspots in bin 1.05/1.06, bin 4.08 and bin 5.03. However, hotspot analysis has some caveats. For example, a hotspot might be observed if a particular inbred line with rare alleles is used as a parent in a large proportion of the populations under study. This may be the case for the inbred line B73. Common alleles may also be hidden in QTL mapping studies when they are present in both parents of most populations, since the alleles would appear to be rare.
Different breeding programmes have produced germplasm with GLS resistance QTL in the same QTL hotspot. For example, the hotspot on chromosome one has been reported from populations with diverse origins [3, 22, 23, 30], including a Mo17 allele (bin 1.05) . This may represent a cluster of R genes or alleles. The QTL in bins 4.06/4.08 from line V0613Y from South Africa , which was mapped to bin 4.08 in a progeny line VP31 , overlaps with a QTL from the ADENT inbred line . V0613Y and ADENT were developed in different breeding programmes on different continents [21, 29]. Interestingly, B73 carries a susceptible allele in this bin region in the ADENT X B73rhm population, whereas it confers a resistant allele in the Val14 X B73 population [21, 22].
The QTL in bin 4.05 appears to contain alleles for GLS resistance from different sources. Balint-Kurti et al.  report a QTL from B73 that was only observed in the IBM population, and not the “Stuber” population. They propose that although these populations have the same parents, there are opposing effects at closely linked loci that cancel out the QTL in the “Stuber” population which has fewer recombination events. Interestingly, Zwonitzer et al.  identified a GLS resistance allele from line Ki14 and not the other parent B73 in this bin (Additional file 1). There is a similar case in bin 8.05, where there is a resistant allele from B73 in the B73 X ADENT population , but a CML52 allele conferring resistance from the B73 X CML52 population .
From the above results from different inbred lines, there is substantial evidence for allelic series at QTL loci for GLS resistance and susceptibility, as have been reported for studies with NAM populations for other maize diseases such as southern corn leaf blight .
Two independent studies [4, 26] identified different alleles for GLS resistance when they used different parents from the Suwan-1 population released in 1975 by Kasetsart University in Thailand, indicating that there are multiple sources of resistance in this population. Zwonitzer et al.  reported GLS resistance alleles from line Ki14 (derived from the Suwan-1 population) on chromosomes four, seven and ten, whereas Zhang et al.  did not report GLS resistance alleles on these chromosomes, but detected GLS resistance alleles from line Y32 (derived from the Suwan-1 population) on chromosome five and eight not present in the former study (Additional file 1).
The proprietary germplasm of Pozar et al.  has GLS resistance alleles that overlap with QTL in bin 1.05/1.06 from line 061 , as well as QTL in bin 7.02/7.03 reported from this line and line NC250A  (Additional file 1). This may indicate that these lines were used in the development of the proprietory parental lines .
There appears to be a relative paucity of GLS resistance QTL on chromosomes three, six, eight, nine and ten (Additional file 1). QTL identified in the CML444 X SC Malawi population that are most likely to be unique were the GLS resistance QTL in bins 6.06/6.07 and 9.06 (Table 2, Additional file 1). The QTL in bin 6.06/6.07 was identified in seven environments and explained on average 13% of the variation in the data, with the resistance allele derived from SC Malawi (Table 2). The QTL in bin 9.06, with resistance also derived from the more susceptible parent, explained 12% of the phenotypic variation. There were no significant epistatic effects between any pairwise combination of QTL (data not shown), as was observed in a GLS QTL study of another RIL population .
Previous authors have observed that there may a link between flowering time and GLS resistance with later maturing plants showing more resistance [21, 23, 25]. CML444 is a later maturing genotype than SC Malawi as reported in , and this was confirmed in the current study in KwaZulu-Natal, where DTA for CML444 was on average five days later than SC Malawi (data not shown). Interestingly, QTL for DTA measured in this study in bins 1.07 and 4.09 (Table 2) corresponded to flowering time QTLs reported for field trials in Mexico and Zimbabwe in . However, these QTL did not overlap with GLS resistance QTLs identified in this study, although the DTA QTL in bin 4.09 is close to the main effect GLS QTL in bin 4.08 observed in multiple environments (Figure 1, Table 2).
C. zeina and not C. zeae-maydis was the pathogen causing GLS in KwaZulu-Natal during the course of this study of the CML444 X SC Malawi population ( and data not shown). Although there are no reports of differential response in maize lines to these two species of fungus , we can conclude that the QTL observed in this population are effective against C. zeina prevalent in South Africa. The only other study where the causal agent is most likely to be C. zeina exclusively is that of Zhang et al.  in Yunnan province of China . It would be interesting to test whether the QTL identified in the CML444 X SC Malawi population are also identified in environments where C. zeae-maydis is the causal agent of GLS. This would be an important consideration for breeding initiatives in different countries. Future work in our group will address this question by conducting field trials of the same maize RIL populations in paired environments where only C. zeae-maydis or only C. zeina are found. Species-specific PCR diagnostic assays developed in our group that can be applied directly from GLS lesions will be used to confirm the prevalent species of fungus in each environment [8, 10].
QTL mapping of the CML444 X SC Malawi RIL population at five sites over three years in KwaZulu-Natal, South Africa allowed the identification of seven QTLs for resistance to GLS caused by C. zeina. The seven QTL were observed in at least two environments and were positioned in common bins in the maize genome. Although analysis of variance indicated that environment had the greatest effect on phenotypic variation, the study succeeded in identifying GLS QTL at high confidence since data was collected from a large number of field trials. Comparison of the QTL from this study with QTL reported from previous studies indicated that QTL from the CML444 X SC Malawi population in bin 4.08 and bin 7.02/7.03 coincided with hotspots of GLS QTL in the maize genome. The QTL in bin 4.08 has previously been reported from the inbred line V0613Y from South Africa . Two novel QTL (bin 6.06/6.07 and 9.06) were discovered in this study, which interestingly had alleles for increased resistance to GLS derived from the susceptible parent SC Malawi.
The financial assistance of the Technology Innovation Agency (TIA), the National Research Foundation (NRF) and the Genomics Research Institute of the University of Pretoria (UP), South Africa, towards this research is hereby acknowledged. Opinions expressed and conclusions arrived at, are those of the authors and are not necessarily to be attributed to TIA, NRF or UP. We thank J-M. Ribaut for assistance with the CML444 X SC Malawi genetic map, and P. Le Roux for assistance with the statistical analysis. We thank the anonymous reviewers for valuable comments and suggestions.
- Latterell FM, Rossi AE: Gray leaf spot of corn - a disease on the move. Plant Disease. 1983, 67: 842-847. 10.1094/PD-67-842.View ArticleGoogle Scholar
- Gevers HO, Lake JK, Hols T: Diallel cross analysis of resistance to gray leaf spot in maize. Plant Disease. 1994, 78: 379-383. 10.1094/PD-78-0379.View ArticleGoogle Scholar
- Pozar G, Butruille D, Silva H, McCuddin Z, Penna J: Mapping and validation of quantitative trait loci for resistance to Cercospora zeae-maydis infection in tropical maize (Zea mays L.). Theor Appl Genet. 2009, 118: 553-564. 10.1007/s00122-008-0920-2.PubMedView ArticleGoogle Scholar
- Zhang Y, Xu L, Fan X, Tan J, Chen W, Xu M: QTL mapping of resistance to gray leaf spot in maize. Theor Appl Genet. 2012, 125: 1797-1808. 10.1007/s00122-012-1954-z.PubMedView ArticleGoogle Scholar
- Ward JMJ, Stromberg EL, Nowell DC, Nutter FW: Gray leaf spot: a disease of global importance in maize production. Plant Disease. 1999, 83: 884-895. 10.1094/PDIS.1922.214.171.1244.View ArticleGoogle Scholar
- Munkvold GP, Martinson CA, Shriver JM, Dixon PM: Probabilities for profitable fungicide use against gray leaf spot in hybrid maize. Phytopathology. 2001, 91: 477-484. 10.1094/PHYTO.2001.91.5.477.PubMedView ArticleGoogle Scholar
- Asea G, Vivek BS, Bigirwa G, Lipps PE, Pratt RC: Validation of consensus quantitative trait loci associated with resistance to multiple foliar pathogens of maize. Phytopathology. 2009, 99: 540-547. 10.1094/PHYTO-99-5-0540.PubMedView ArticleGoogle Scholar
- Korsman J, Meisel B, Kloppers F, Crampton B, Berger D: Quantitative phenotyping of grey leaf spot disease in maize using real-time PCR. Eur J Plant Pathol. 2012, 133: 461-471. 10.1007/s10658-011-9920-1.View ArticleGoogle Scholar
- Wang J, Levy M, Dunkle LD: Sibling species of cercospora associated with gray leaf spot of maize. Phytopathology. 1998, 88: 1269-1275. 10.1094/PHYTO.19126.96.36.1999.PubMedView ArticleGoogle Scholar
- Meisel B, Korsman J, Kloppers F, Berger DK: Cercospora zeina is the causal agent of grey leaf spot disease of maize in southern Africa. Eur J Plant Pathol. 2009, 124: 577-583. 10.1007/s10658-009-9443-1.View ArticleGoogle Scholar
- Crous PW, Groenewald JZ, Groenewald M, Caldwell P, Braun U, Harrington TC: Species of Cercospora associated with grey leaf spot of maize. Stud Mycol. 2006, 55: 189-197.PubMedPubMed CentralView ArticleGoogle Scholar
- Carson ML, Goodman MM, Williamson SM: Variation in aggressiveness among isolates of cercospora from maize as a potential cause of genotype-environment interaction in gray leaf spot trials. Plant Disease. 2002, 86: 1089-1093. 10.1094/PDIS.2002.86.10.1089.View ArticleGoogle Scholar
- Dunkle LD, Levy M: Genetic relatedness of African and United States populations of Cercospora zeae-maydis. Phytopathology. 2000, 90: 486-490. 10.1094/PHYTO.2000.90.5.486.PubMedView ArticleGoogle Scholar
- Okori P, Fahleson J, Rubaihayo PR, Adipala E, Dixelius C: Assessment of genetic variation among East African Cercospora zeae-maydis. African Crop Science. 2003, 11: 75-85.Google Scholar
- Coates ST, White DG: Inheritance of resistance to gray leaf spot in crosses involving selected resistant inbred lines of corn. Phytopathology. 1998, 88: 972-982. 10.1094/PHYTO.19188.8.131.522.PubMedView ArticleGoogle Scholar
- Sibiya J, Tongoona P, Derera J, Rij N: Genetic analysis and genotype X environment (G X E) for grey leaf spot disease resistance in elite African maize (Zea mays L.) germplasm. Euphytica. 2012, 185: 349-362. 10.1007/s10681-011-0466-2.View ArticleGoogle Scholar
- Huff CA, Ayers JE, Hill RR: Inheritance of resistance in corn (Zea mays) to gray leaf spot. Phytopathology. 1988, 78: 790-794. 10.1094/Phyto-78-790.View ArticleGoogle Scholar
- Poland JA, Balint-Kurti PJ, Wisser RJ, Pratt RC, Nelson RJ: Shades of gray: the world of quantitative disease resistance. Trends Plant Sci. 2009, 14: 21-29. 10.1016/j.tplants.2008.10.006.PubMedView ArticleGoogle Scholar
- Roux F, Gao L, Bergelson J: Impact of initial pathogen density on resistance and tolerance in a polymorphic disease resistance gene system in Arabidopsis thaliana. Genetics. 2010, 185: 283-291. 10.1534/genetics.109.112383.PubMedPubMed CentralView ArticleGoogle Scholar
- Van der Linden L, Bredenkamp J, Naidoo S, Fouche-Weich J, Denby KJ, Genin S, Marco Y, Berger DK: Gene-for-gene tolerance to bacterial wilt in arabidopsis. Mol Plant Microbe Interact. 2013, 26: 398-406. 10.1094/MPMI-07-12-0188-R.PubMedView ArticleGoogle Scholar
- Bubeck DM, Goodman MM, Beavis WD, Grant D: Quantitative trait loci controlling resistance to gray leaf spot in maize. Crop Sci. 1993, 33: 838-847. 10.2135/cropsci1993.0011183X003300040041x.View ArticleGoogle Scholar
- Saghai Maroof AS, Yue YG, Xiang ZX, Stromberg EL, Rufener GK: Identification of quantitative trait loci controlling resistance to gray leaf spot disease in maize. Theoret Appl Genetics. 1996, 93: 539-546. 10.1007/BF00417945.View ArticleGoogle Scholar
- Clements MJ, Dudley JW, White DG: Quantitative trait loci associated with resistance to gray leaf spot of corn. Phytopathology. 2000, 90: 1018-1025. 10.1094/PHYTO.2000.90.9.1018.PubMedView ArticleGoogle Scholar
- Gordon SG, Bartsch M, Matthies I, Gevers HO, Lipps PE, Pratt RC: Linkage of molecular markers to Cercospora zeae-maydis resistance in maize. Crop Sci. 2004, 44: 628-636. 10.2135/cropsci2004.0628.Google Scholar
- Balint-Kurti PJ, Wisser R, Zwonitzer JC: Use of an advanced intercross line population for precise mapping of quantitative trait loci for gray leaf spot resistance in maize. Crop Sci. 2008, 48: 1696-1704. 10.2135/cropsci2007.12.0679.View ArticleGoogle Scholar
- Zwonitzer JC, Coles ND, Krakowsky MD, Arellano C, Holland JB, McMullen MD, Pratt RC, Balint-Kurti PJ: Mapping resistance quantitative trait loci for three foliar diseases in a maize recombinant inbred line population - evidence for multiple disease resistance?. Phytopathology. 2010, 100: 72-79. 10.1094/PHYTO-100-1-0072.PubMedView ArticleGoogle Scholar
- Chung CL, Poland J, Kump K, Benson J, Longfellow J, Walsh E, Balint-Kurti P, Nelson R: Targeted discovery of quantitative trait loci for resistance to northern leaf blight and other diseases of maize. Theor Appl Genet. 2011, 123: 307-326. 10.1007/s00122-011-1585-9.PubMedView ArticleGoogle Scholar
- Wisser RJ, Kolkman JM, Patzoldt ME, Holland JB, Yu J, Krakowsky M, Nelson RJ, Balint-Kurti PJ: Multivariate analysis of maize disease resistances suggests a pleiotropic genetic basis and implicates a GST gene. PNAS. 2011, 108: 7339-7344. 10.1073/pnas.1011739108.PubMedPubMed CentralView ArticleGoogle Scholar
- Gevers HO, Lake JK: GLS1 - a major gene for resistance to grey leaf spot in maize. S Afr J Sci. 1994, 90: 377-379.Google Scholar
- Lehmensiek A, Esterhuizen AM, van Staden D, Nelson SW, Retief AE: Genetic mapping of gray leaf spot (GLS) resistance genes in maize. Theor Appl Genet. 2001, 103: 797-803. 10.1007/s001220100599.View ArticleGoogle Scholar
- Juliatti FC, Pedrosa MG, Silva HD, da Silva JVC: Genetic mapping for resistance to gray leaf spot in maize. Euphytica. 2009, 169: 227-238. 10.1007/s10681-009-9943-2.View ArticleGoogle Scholar
- Liu KJ, Xu XD: First report of gray leaf spot of maize caused by Cercospora zeina in China. Plant Disease. 2013, 97: 1656-View ArticleGoogle Scholar
- Messmer R, Fracheboud Y, Banziger M, Vargas M, Stamp P, Ribaut JM: Drought stress and tropical maize: QTL-by-environment interactions and stability of QTLs across environments for yield components and secondary traits. Theor Appl Genet. 2009, 119: 913-930. 10.1007/s00122-009-1099-x.PubMedView ArticleGoogle Scholar
- Fox J, Weisberg S: An R companion to Applied Regression, 2nd edn. 2011, Thousand Oaks, USA: Sage PublicationsGoogle Scholar
- R Development Core Team: R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing. 2014, Vienna, Austria, ISBN 3-900051-07-0, URL http://www.R-project.org/Google Scholar
- Manly KF, Cudmore RH, Meer JM: Map Manager QTX, cross-platform software for genetic mapping. Mammalian Genome. 2001, 12: 930-932. 10.1007/s00335-001-1016-3.PubMedView ArticleGoogle Scholar
- Voorrips RE: MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered. 2002, 93: 77-78. 10.1093/jhered/93.1.77.PubMedView ArticleGoogle Scholar
- Wang S, Basten CJ, Zeng Z-B: Windows QTL Cartographer 2.5_011. Department of Statistics, North Carolina State University, Raleigh, NC. 2012, http://statgen.ncsu.edu/qtlcart/WQTLCart.htm,Google Scholar
- Doerge RW, Churchill GA: Permutation tests for multiple loci affecting a quantitative character. Genetics. 1996, 142: 285-294.PubMedPubMed CentralGoogle Scholar
- Basten CJ, Weir BS, Zeng Z-B: QTL Cartographer version 2.5_002. North Carolina State University, Raleigh, NC, USA. 2007Google Scholar
- Balint-Kurti PJ, Krakowsky MD, Jines MP, Robertson LA, Molnar TL, Goodman MM, Holland JB: Identification of quantitative trait loci for resistance to southern leaf blight and days to anthesis in a maize recombinant inbred line population. Phytopathology. 2006, 96: 1067-1071. 10.1094/PHYTO-96-1067.PubMedView ArticleGoogle Scholar
- Wisser RJ, Balint-Kurti PJ, Nelson RJ: The genetic architecture of disease resistance in maize: a synthesis of published studies. Phytopathology. 2006, 96: 120-129. 10.1094/PHYTO-96-0120.PubMedView ArticleGoogle Scholar
- Shi L, Li X, Hao Z, Xie C, Ji H, Lu X, Zhang S, Pan G: Comparative QTL mapping of resistance to gray leaf spot in maize based on bioinformatics. Agr Sci China. 2007, 6: 1411-1419. 10.1016/S1671-2927(08)60002-4.View ArticleGoogle Scholar
- Luo S, Zhang Y, Hu Q, Chen J, Li K, Lu C, Liu H, Wang W, Kuang H: Dynamic nucleotide-binding site and leucine-rich repeat-encoding genes in the grass family. Plant Physiology. 2012, 159: 197-210. 10.1104/pp.111.192062.PubMedPubMed CentralView ArticleGoogle Scholar
- Kump KL, Bradbury PJ, Wisser RJ, Buckler ES, Belcher AR, Oropeza-Rosas MA, Zwonitzer JC, Kresovich S, McMullen MD, Ware D, Balint-Kurti PJ, Holland JB: Genome-wide association study of quantitative resistance to southern leaf blight in the maize nested association mapping population. Nat Genet. 2011, 43: 163-168. 10.1038/ng.747.PubMedView ArticleGoogle Scholar
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