Similarity of putative insulin regulatory sequence with known insulin regulatory sequence of rat phosphoenolpyruvate carboxykinase (PEPCK) All the sequences shown are from the coding strand of the respective gene promoter. The 5' end positions are expressed relative to the transcription start site (GenBank accession: M90298 for human liver glucokinase; M24943 for rat liver glucokinase; K03243 for rat PEPCK). Human PEPCK has two copies of insulin regulatory sequences in its promoter and the transcription start site has not been mapped yet (Chiu KC, unpublished data). The underlined A in the variant human liver glucokinase is the position where the G-to-A substitution occurs. The blocked area is conserved between human and rat and also between PEPCK and glucokinase.