Figure 6
Accumulation of FXR1P82,84 is an early event and coincides with expression of different myogenetic markers. Protein (40 ÎĽg) and RNA (5 ÎĽg) were prepared at the indicated times and subjected to immunoblot (Western) and Northern analyses, respectively. Exposure times for Western analyses were between 15 and 30 sec. Northern blots were exposed for 36 h while the 81 bp insert required prolonged exposure up to 72 h due to the short probe used. hps: hours post-stimulation after serum deprivation.