Table 1 Typing of Amh, Dax1 and Dmrt1 genes and alleles by sequence polymorphisms in rainbow trout crosses.
From: Mapping of five candidate sex-determining loci in rainbow trout (Oncorhynchus mykiss)
Gene | Accession numbers | Parent | Site of polymorphism | Polymorphism | Method of genotyping |
|---|---|---|---|---|---|
Amh | FJ609190 | Arlee | 82–85 of fifth intron | AAT(T) | PCR-RFLP Tsp509I |
FJ609189 | OSU | 82–85 of fourth intron | AAT(G) | ||
Dax1 | FJ617280 | HC | -10 of 5' UTR | G | Taqman assay |
FJ617279 | OSU | -10 of 5' UTR | A | ||
Dmrt1 | -- | HC | 743 of first intron | GTA(C) | PCR-RFLP Rsa1 |
FJ617281 | OSU | 743 of first intron | GTA(A) |