Mapping of five candidate sex-determining loci in rainbow trout (Oncorhynchus mykiss)

BMC Genomic Data

Table 5 Primer sequences for amplifying non-coding regions of candidate sex-determining genes in rainbow trout.

Gene	Cloning/Library screening primers	Marker position	Mapping primers
Sox6	F: TTCACAGGCAGCAAGACCAG R: AACAGCGCTGTGGAGTTCAG	4^th intron	MF: TTCACAGGCAGCAAGACCAG MR: AACAGCGCTGTGGAGTTCAG
			SF: CTAGAGCTGCGGATGTTGTAAC SR: TGAGCCCACTGGCAGGTGTTC
Amh	F: CATCACTTTCACCAGTCACTC R: TCGGTACTGCGTCTCACTG	4^th intron	MF: CATCACTTTCACCAGTCACTC MR: TCGGTACTGCGTCTCACTG
D ax1	LF: CTCCGGTCACCGCAGGTTAC LR: AGGATCCGTTGCAACATGC	5' UTR	TF: CGAGCAGCACCCGATGTAG TR: CCTCGCGAGTGGCCAT
	F: ACCTACAGCACCGAATATCAC R: GTTGTTGCCTTAGCTCAAGC		P: CACGTGGTGCGCG P: CACGTGGCGCGCG
Dmrt1	F: AGGAACCACGGCTACGTGT R: CAACCTCCTGACTGGACAG	1^st intron	MF: CAGAAATGCAAACTGATCGC MR: CAACCTCCTGACTGGACAG

F and R, forward and reverse primers used for amplifying genomic fragments that were later cloned and sequenced; LF and LR, forward and reverse primers used for library screening; MF and MR, forward and reverse primers used for mapping each respective fragment; SF and SR; forward and reverse primers used for genotyping by size polymorphisms; TF and TR: forward and reverse primers used for genotyping by Taqman assay. P: Sequences of the probes used for genotyping by Taqman assay.

ISSN: 2730-6844