Genotyping human ancient mtDNA control and coding region polymorphisms with a multiplexed Single-Base-Extension assay: the singular maternal history of the Tyrolean Iceman

Table 2 Multiplex Single Base Extension results for the HB50 bone extracts of the Iceman's DNA using the K1 multiplex.

Sample
rCRS	MP	T	T	T	C	C	T	G	C	T	A	T
HB50	K1	C 16224C	C 16311C	C 16362C	C497T	C498 elC	C1189C	G5913A	T8137T	C11299C	G12308G	C12705C

For the sake of consistency with Table 1, alleles read from the forward DNA strand, relative to the revised Cambridge Reference Sequence (rCRS) [23, 24], unlike Figures 2 and 3 that show the state according to the actual strand targeted by the SBE assay. These results are identical to the cloned singleplex results shown in Table 1, demonstrating consistent genotyping of the Iceman by different methods. As all these results were obtained in a single multiplex reaction, credibility is given to those reactions returning the rCRS. Such results in a singleplex scenario could be explained by contamination of an individual assay by any non hg K mtDNA source.

ISSN: 2730-6844