Haploid cells were grown overnight in YPD rich medium. 106 cells were plated onto selective medium lacking uracil. Colonies were counted after 3 days. The mean frequency and standard deviation obtained from at least 4 independent cultures are shown.
aOnly 2 independent cultures were tested.
bCultures were grown in supplemented minimal medium containing raffinose. Where indicated, galactose was added to 1.5% and culture was grown for 1.3 doublings prior to plating onto selective medium. Diluted cultures were also plated to YPD to score viable cell density. Ura+ frequency was computed per viable cell plated. A paired t-test indicates that the frequencies were not different (2-tailed, P > 0.1).