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Table 1 DNA Recovery from Two 3 mm Guthrie Card Punches

From: Whole genome microarray analysis, from neonatal blood cards

sample ID

Yield (ng)

Conc (ng/uL)

Specimen Name

Collection Date

1

261.7

26

530011

1995

2

203.37

20

529979

1995

3

162.33

16

529969

2003

4

312.9

31

529943

2003

5

380.43

38

529844

1994

6

212.16

21

529895

1994

7

319.9

32

520908

2001

8

188.41

19

529809

2001

9

246.88

65

MOD881

1993

10

244.55

65

MOD892

1993

11

225.47

23

529874

2003

12

131.31

13

529810

2003

13

391.96

50

MOD919

1998

14

367.67

48

MOD937

1998

15

161.23

16

529852

2000

16

264.59

26

529906

2000

17

255.67

57

MOD920

1995

18

226.43

65

MOD975

1995

19

268.02

69

MOD986

1995

20

234.94

53

MOD001

1995

21

350.78

35

530127

1995

22

339.53

34

530128

1991

23

224.1

22

529236

1992

24

274.61

27

529237

1997

overall average

<260 ng>

<26 ng/uL>

 

<1997>

Microarray samples

<280 ng>

<59 ng/uL>

 

<1995>

  1. DNA concentration was measured via PicoGreen analysis. Samples in red refer to those specimens used for microarray analysis, based on the requirement that their DNA concentration exceed 50 ng/uL to accommodate Illumina hybridization chemistry. Notice that the average DNA yield (in nanograms) for the 8 specimens used for microarray analysis and their average age did not deviate significantly from the average of all 24. However, due to volume variability in the final concentrated DNA sample, the mass concentration of the 8 specimens used for microarray analysis (59 ng/uL) was approximately twice that of the average over the entire set of twentyfour (26 ng/uL).