Skip to main content

Table 4 PCR sequence verification for 16 single-feature polymorphisms (SFPs)

From: Single-feature polymorphism discovery by computing probe affinity shape powers

SFPs1

Tissue2

Genotype3

Shape4

Weight5

PCR6

Map info7

Contig16609_at10

3

U

4

85.45

+

NA

Contig4329_at3

4

B

4

70.77

+

6H,L, 67.7 cM

Contig4329_at6

2

B

4

71.33

+

6H,L, 67.7 cM

Contig5339_s_at8

4

B

4

65.08

+

6H,L, 70 cM

Contig7178_s_at8

4

U

3.67

50.82

+

6H,L, 70 cM

Contig4107_x_at11

4

U

2

23.01

+

NA

Contig9366_at10

3

U

2.67

39.79

+

NA

Contig159_at1

4

U

2

15.03

+

NA

Contig159_at6

3

U

1

3.78

+

NA

Contig9298_at7

2

U

1

3.64

+

NA

Contig14687_at7

3

B

3

37.18

+

6H,L, 71.1 cM

HVSMEg0016A12r2_s_at5

4

B

4

75.24

+

6H,S, 49.4 cM

HVSMEg0016A12r2_s_at7

3

B

2

16.13

+

6H,S, 49.4 cM

HVSMEn0016F09r2_s_at7

1

U

1

4.48

+

6H,L, 71.1 cM

Contig2856_x_at6

3

U

1.67

8.16

+

6H,L, 60.2 cM

Contig8825_at7

3

B

1

5.48

-

3H,L, 55.6 cM

  1. 1Indicates the contig and probe that the SFP was detected.
  2. 2The tissue column indicates the number of tissues out of four where the SFP was discovered.
  3. 3Genotype represents the SFP detected in Bowman (B) or in Bowman-cul2 (U).
  4. 4The shape power and 5weight score were calculated as described in the Materials and Methods section.
  5. 6The SFP was verified (+) or not verified (-) by sequencing PCR products.
  6. 7SFP map information was derived from barley EST Assembly #35 at the HarvEST website http://harvest.ucr.edu/[50]. Map locations on chromosome 6H or others were designated as long (L) or short (S) arm, at length cM, unless mapping information was not available (NA).