Determining the effectiveness of High Resolution Melting analysis for SNP genotyping and mutation scanning at the TP53 locus

Table 1 Oligonucleotide primer sequences used for comparison of HRM and sequencing sensitivity and specificity.

Amplicon	Forward sequence 5'>3'	Reverse sequence 5'>3'	Location	size
3	CGGGACGTGAAAGGTTAGAA	TTTTGGGGTGGAAAATTCTG	promoter	653
39	TGGCCATCTACAAGCAGTCA	ACACGCAAATTTCCTTCCAC	exon5-intron5	211
40	CATGAGCGCTGCTCAGATAG	CAGTTGCAAACCAGACCTCA	exon6	234
41	GTGGAAGGAAATTTGCGTGT	TTGCACATCTCATGGGGTTA	intron6	212
43	TGGCTCTGACTGTACCACCA	TCTACTCCCAACCACCCTTG	intron 7	371
44	CTGGAAGACTCCAGGTCAGG	AGCTGTTCCGTCCCAGTAGA	intron7	383
46	GCGCACAGAGGAAGAGAATC	TGAAAGCTGGTCTGGTCCTT	intron9	452
47	GCAGTGATGCCTCAAAGACA	GCAGGCTAGGCTAAGCTATGA	intron9	280
48	TGACTTTGCCTGATACAGATGC	TAGCTACTGGGGAGGCAGAG	intron9	596
49	GGCCTGCCTAGCCTACTTTT	GTAGCAGGCGCTTGTAGTCC	intron9	578
50B	GACTACAAGCGCCTGCTACC	TTTCATGCAACCATGCTGTT	intron9	614
51	CCCTACAGTTGGGCAAAGTC	CGACTGTGCCTCGTTTCTTT	intron9	491
52A	CCTGGGCGATAGAGTGAGAC	GGCTGGACTCAAACTCTTGG	intron9	134
52B	GTCGCATGCACATGTAGTCC	CTTGAGTTCCAAGGCCTCAT	intron9	635
53	ACTTCTCCCCCTCCTCTGTT	CCTGGGTTTGGATGTTCTGT	exon10-intron10	348
55	TATACTCAGCCCTGCCATGC	GGACTTCAGGTGGCTGTAGG	intron10	603
57	TTTGGGTCTTTGAACCCTTG	GTGGTTTCAAGGCCAGATGT	exon11 (3'UTR)	400
58	GGCCCACTTCACCGTACTAA	AAGCGAGACCCAGTCTCAAA	exon11 (3'UTR)	485
59	AAGGAAATCTCACCCCATCC	AAATGCAGATGTGCTTGCAG	exon11 (3'UTR)	456
60	TTGAGACTGGGTCTCGCTTT	CAGTCTCCAGCCTTTGTTCC		566
61	AAAACTTTGCTGCCACCTGT	ATCCTGCCACTTTCTGATGG		415
62	GCCTCTCACCAAGGATTACG	CCTGGACAGTAGCACCCACT		535

ISSN: 2730-6844