Table 2 Primer and enzymes used for genotyping of SNP
SNP | Method | Primer | Enzyme | Alleles | |
|---|---|---|---|---|---|
CDH1_c.2102C>T | RFLP | forward | 5'-CAT AGA CAA CCA GAA CAA AGA C | Eco 57I | A/G |
reverse | 5'-TGG ACC TCT GGG GAG ACT G | (Fermentas Life Sciences) | |||
HAMP_c.366+109G>A | RFLP | forward | 5'-AGA CAC CCA CTT TCC CAT C | Csp 6I | A/G |
reverse | 5'-AGC TCC ACA GTC TCT TCT C | (Fermentas Life Sciences) | |||
PVRL2_c.-1268G>C | RFLP | forward | 5'-AAT GCC AGT CAA TCA CAG TCT C | Hinc II | G/C |
reverse | 5'-GGA TTC TAC ACC CGC TGC TC | (Fermentas Life Sciences) | |||
PVRL2_c.392G>A | RFLP | forward | 5'-TTC CTC AAA CTG TCT TAT CTG G | Hin 6I | A/G |
reverse | 5'-GTG TAG TTG CCC TCG TCC TC | (Fermentas Life Sciences) | |||
CALM3_c.3+1795C>T | RFLP | forward | 5'-TTG AGA GAA AAC CAG CAG AC | Bse YI | C/T |
reverse | 5'-CCA GGC AGC AGT GTT AGA | (New England Biolabs Inc.) | |||
CALM3_c.3+1678C>T | pyro-sequencing | forward | 5'-GAG CCC TCC CTG AGT GCT TC | C/T | |
reverse | 5'-AGC GGC TGC CTG TTC TCC | ||||
sequencing | 5'-AGG ATG GCT GCA CAC | ||||
HAMP_c.86+430G>A | pyro-sequencing | forward | 5'-AAA AGA TGG TGG GAG AGT AAT GG | A/G | |
reverse | 5'-CCT CTG CAC TTG CCT GTA AGA CTT | ||||
sequencing | 5'-CCA AAT AGG TCA AAT AAC A |