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Figure 4 | BMC Genetics

Figure 4

From: High resolution analysis of the human transcriptome: detection of extensive alternative splicing independent of transcriptional activity

Figure 4

Lim Domain 7 splicing alteration. Lim domain 7 (LMO7) was selected from a set of genes that displayed little difference at the gene level between samples A and B, but had event ratios that were significantly higher than 3 fold between these samples, indicating a splicing alteration. (A) Using the SpliceArrayâ„¢ portal (http://portal.splicearray.com) and the associated UCSC browser feature, the event is illustrated with the variant sequence used to identify the event (red), the reference sequence (black), the probe target regions (blue), and the PCR primers (arrowheads) used to validate the event. (B) RT-PCR results for the event validation using the primers indicated in A. R = reference and V = variant (C) Protein domain analysis was done using CDART [38] and shows the C terminal Lim domain is missing from the splice variant. (Note that Event ID 4008.010 = Entrez Gene ID.ID1 and probe sets are additionally labeled with F, T, B, C, D, or E.)

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