High resolution analysis of the human transcriptome: detection of extensive alternative splicing independent of transcriptional activity

Table 3 Event analysis of genes grouped by overall gene expression.

Gene Expression Level (median of F and T)		Event level (B/E ratio)
Fold Change Set	Total Genes	Total Events	Subset of events with ≥3 Fold Change
x ≥ 3 or ≤ -3	1844	13323	7883
-1.2 ≤ x ≤ 1.2	4434	32917	3012

Genes were classified into two groups: genes that were not differentially expressed between samples A and B (fold changes within the range of -1.2 ≤ x ≤ 1.2), and genes with a greater than 3-fold change (x ≤ -3 or x ≥ 3). In order to determine the differential splicing occurring for each gene, the ratio of the long form probe (B) and the short form probe (E) was calculated, and events were selected where the ratio was differentially expressed between samples A and B by greater than 3-fold.

ISSN: 2730-6844