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Table 8 Estimated reagent and sequencing costs

From: Automated DNA mutation detection using universal conditions direct sequencing: application to ten muscular dystrophy genes

Reagent

Cost/well

Extended cost Plate 1

Extended cost Plate 2 or 3

Assay

$0.03

  

Fast Start

$0.34

  

dNTPs

$0.04

  

10× Buffer

$0.02

  

Sub Total

$0.43

  

X6/5*

 

$0.52

 

X8/7#

  

$0.49

Plastic ware

$0.06

$0.06

$0.06

QIAxcel system

$0.25

$0.25

$0.25

AmPure

$0.07

$0.07

$0.07

Total PCR

reagents

 

$0.90/patient PCR

$0.87/patient PCR

Big Dye

$0.90

$1.80/patient PCRΘ

$1.80/patient PCR

Buffer

$0.06

$0.12/patient PCR

$0.12/patient PCR

Plate

$0.01

$0.02/patient PCR

$0.02/patient PCR

CleanSeq

$0.30

$0.60/patient well

$0.60/patient PCR

Plate

$0.01

$0.02/patient PCR

$0.02/patient PCR

Total per patient well

 

$3.46

$3.43

Total per patient

 

$442.88

$329.28

Add ~20% for waste

 

$532.00

$395.00

  1. * for one negative control per 5 patients on plate 1; # one negative control per 7 patients on plates 2 or 3; Θ for both Forward and Reverse sequence/PCR.
  2. 20% waste value is a conservative estimate of waste established over many years and used by our clinical and research labs in calculating material waste. It includes remaining reagents in large cocktail batches and possible manual repeats.