Figure 4

Bisulfite sequencing results for normal dog 1, and dogs 5, 12, and 19 with NHL. The corresponding DNA sequence is above with each CpG dinucleotide marked in bold. Open circles denote unmethylated CpG dinucleotides and black are methylated. Five clones for each dog were submitted for sequencing. Note the relatively lower methylation density for the normal dog. The high frequency of methylation at the HpyCh4IV cut-site in all dogs, including the normal dog, suggests that this CpG behaves as a genomic CpG dinucleotide and is routinely methylated. Note the minimal methylation in the right half each set of clones where the Sp1 sites reside.