Table 6 Restriction enzymes
SNP | Enzyme | Specificity* 5' → 3' | Primer pair | Units per reaction | Incubation time |
|---|---|---|---|---|---|
ss161109995 | Bsp143 II | PuGC GCPy | 3 | 0.5 | 14 h |
ss161109997 | Bsh1236 I | CGC G | 5 | 0.5 | 14 h |
ss161109998 | Eco72 I | CACG TG | 5 | 5 | 3 h |
ss161110009 | Esp3 I | C GTCTC | 12 | 0.5 | 14 h |
ss161110010 | EcoO109 I | PuGG NCCPy | 15 | 0.5 | 14 h |