Effectiveness of 10 polymorphic microsatellite markers for parentage and pedigree analysis in plateau pika (Ochotona curzoniae)

Table 1 Parameters of the 10 microsatellite loci

Locus	k	N	Hobs	Hexp	PIC	NE-1P	NE-2P	NE-PP	F(null)	NE-I	NE-SI	HW	PD	PE	CPE
EU518185	7	15	0.933	0.834	0.779	0.560	0.383	0.203	-0.076	0.047	0.349	NS	0.895	0.887	0.887
EU518186	11	14	0.857	0.884	0.836	0.452	0.290	0.121	0.000	0.083	0.382	**	0.927	0.880	0.98644
EU518196	11	13	0.846	0.905	0.857	0.413	0.258	0.100	0.002	0.073	0.372	NS	0.906	0.872	0.9982643
EU518194	10	16	0.938	0.875	0.83	0.466	0.301	0.131	-0.050	0.104	0.415	NS	0.932	0.773	0.999606
EU518192	8	14	0.857	0.831	0.775	0.560	0.383	0.198	-0.035	0.021	0.309	NS	0.900	0.760	0.9999054
EU518193	8	15	0.933	0.784	0.723	0.630	0.452	0.262	-0.111	0.137	0.435	NS	0.828	0.745	0.9999759
EU518189	6	16	0.938	0.823	0.767	0.578	0.400	0.218	-0.084	0.057	0.356	NS	0.877	0.662	0.9999918
EU518184	5	16	0.688	0.690	0.623	0.743	0.569	0.383	-0.041	0.037	0.335	NS	0.827	0.463	0.9999956
EU518187	8	14	0.714	0.810	0.753	0.588	0.409	0.218	0.058	0.052	0.351	NS	0.907	0.437	0.9999975
EU518191	8	15	0.600	0.809	0.752	0.593	0.415	0.228	0.143	0.087	0.39	NS	0.803	0.351	0.9999984

Locus: Named from GenBank accession; k: Number of alleles at the locus; N: Number of individuals typed at the locus; Hobs: Observed heterozygosity; Hexp: Expected heterozygosity; PIC: Polymorphic information content; NE-1P: Average non-exclusion probability for one candidate parent; NE-2P: Average non-exclusion probability for one candidate parent given the genotype of a known parent of the opposite sex; NE-PP: Average non-exclusion probability for a candidate parent pair; F(Null): Estimated null allele frequency; PD: Power of discrimination; PE: Power of exclusion; CPE: Combined probability of exclusion; NS = not significant, ** = significant at the 1% level; NE-I: Average non-exclusion probability for identity of two unrelated individuals; NE-SI: Average non-exclusion probability for identity of two siblings.

ISSN: 2730-6844