Identification of the complete 5' end region of a novel muscle transcript of the ankyrin 1 gene following 5' RACE-PCR, cloning and sequencing. a. Newly identified transcription start site (TSS), start codon (ATG) and stop codon (TGA) are in bold and underlined. Alternative, previously published TSS and ATG (from GenBank: AF222766.1) are underlined. The previously known exon1 is shaded and the internal primer is also underlined. b. Protein sequence of the complete 5'end region of a novel muscle transcript. Recognised protein sequence is shaded.