Identification of the TSS of the LRP5 gene and the putative transcription factor binding sites in human LRP5 promoter. (A) The transcription start site (TSS) was determined by primer extension, and the extended product is indicated by the arrow. (B) Nucleotide sequence surrounding the 5' end of human LRP5. The numbering of the sequence is relative to TSS. Distinct DNA binding sites for transcription factors are indicated (underlined). The numbers above the sequence referred to the 5' position of serial deletion constructs. The primers used in ChIP assay are indicated in italic and bold.