Table 1 The oligonucleotide sequence of primers used in PCR
From: Sp1 and KLF15 regulate basal transcription of the human LRP5 gene
Name | Sequence(5'→3') | Strand | Location |
|---|---|---|---|
LRP5F | GCCTGACTGAGGAGCTGAAG | sense | -2381 ~ -2361 |
LRP5R | GCTGCCTCCATGTTGTCC | antisense | + 105 ~ +125 |
LRP5R/HindIII | CCCAAGCTT GCTGCCTCCATGTTGTCC | antisense | +105 ~ +125 |
-1901 F/KpnI | GACGGTACC AGCTACGATCACACCACTAC | Sense | -1901 ~ -1881 |
-1594 F/KpnI | GACGGTACC GGCTGCCATGAGATCAGGTG | Sense | -1594 ~ -1574 |
-1442 F/KpnI | GACGGTACC AGGCTGTGCTGGTAGAGCTG | sense | -1442 ~ -1422 |
-1309 F/KpnI | GACGGTACC CTGTACCTTCTACCACCAGC | Sense | -1309 ~ -1279 |
-1167 F/KpnI | GACGGTACC TTGGTAGCAGCTCAAGTGTC | Sense | -1167 ~ -1147 |
-1085 F/KpnI | GACGGTACC TTCTTCATGGCCTGTCAATG | Sense | -1085 ~ -1065 |
-953 F/KpnI | GACGGTACC AATCTGGAGGAACACACACG | Sense | -953 ~ -933 |
-359 F/KpnI | GACGGTACC TGAAACTGCCTCGCTGTGTG | Sense | -359 ~ -339 |
-219 F/KpnI | GACGGTACC GCCAAGCCTCCCGGAGGCAC | Sense | -219 ~ -199 |
-167 F/KpnI | GACGGTACC CCGCGGGGACCTGCGCCGCT | Sense | -167 ~ -147 |
-72 F/MluI | CGACGCGT GGCGGCGGGGGGCGGAGAAG | Sense | -72 ~ -52 |
-53 F/MluI | CGACGCGT GCGGCGCGGCTTCCGCTCCCG | Sense | -53 ~ -33 |
-40 F/KpnI | GACGGTACC CGCTCCCGCGCGCCCAGCTC | Sense | -40 ~ -20 |
-72MT1/MluI | CGACGCGT GGCGGCTTT GGGCGGAGAAG | Sense | -72 ~ -52 |
-72MT2/MluI | CGACGCGT GGCGGCGGGGGGTT GAGAAGCGGCG | Sense | -72 ~ -52 |
-72MT12/MluI | CGACGCGT GGCGGCTTT GGGTT GAGAAGCGGCG | Sense | -72 ~ -52 |