Figure 5

Relative quantities of expression of NCF1 and its pseudogene. The relative proportion of gene expression of NCF1 and NCF1Ψs (GTGT or GT containing transcripts) was measured by genotyping the cDNA samples from LCLs of 14 human individuals at the signature GT deletion. Two pseudogenes collectively contribute to lesser amount of transcript than from one NCF1 gene copy alone (0.3-0.7):1 [NCF1Ψ/NCF1, the [GT]/[GTGT] ratio) (yellow bars). The [GT]/[GTGT] ratios in genomic DNA are shown by the green bars. Three independent experiments were performed in duplicates.