Figure 1From: Male-like sexual behavior of female mouse lacking fucose mutarotaseTargeted Disruption of the FucM Gene. (A) Schematic diagram for the targeted disruption of the FucM gene. The structures of the targeting vector, wild-type, and disrupted FucM alleles are shown. The solid boxes represent the exons (a total of six in the wild-type). The restriction sites used for constructing the targeting vector and for analysis by Southern blot are EcoRV, BssHI, ScaI, and KpnI. A puromycin expressing cassette was inserted between the EcoRV and ScaI sites, eliminating exons 2-4 and the EcoRV site of the WT gene. (B) Southern analysis for genomic DNA from the wild-type (+/+), heterozygous (+/-) and FucM null (-/-) mice. The expected sizes of the wild-type and disrupted FucM alleles are 10 and 16 Kb, respectively, which were detected by Southern blotting with EcoRV digestion and hybridization using the probe 1 shown in (A). (C) The wild-type (primer 1 and 2) and disrupted alleles (primer 3 and 4) were also detected by PCR.Back to article page