Gene cassette transcription in a large integron-associated array

BMC Genomic Data

Table 1 PCR primers (Sigma Aldrich, Castle Hill, Australia)

Name,	Binding site	Sequence	Usage
YB3 6FAM	DAT722 attC rev	GAATCMCTCTTRARYDSTTTGT	attC PCR
YB4 6FAM	DAT722 attC fwd	TCACMCCTTARYDSGGCGTTA	attC PCR, Promoter isolation
MazG f	Cassette 21 ORF fwd	GTTATCTGAGTTACAAAGTC	Method validation, Promoter isolation
MazG r	Cassette 21 ORF rev	TCCTATCGGTTGTACTTAAC	Method validation, Promoter isolation
VhC 21f	Cassette 21 ORF fwd	TATGCGCCAGAGCAATCTGAACACTAT	Promoter isolation
VhC 21r	Cassette 21 ORF rev	CCGTGAATATTGGCTAGAGCACAAACA	Promoter isolation
VhC 89f	Cassette 89 ORF fwd	ATCAGAAATTGAAGACTTGC	Method validation
VhC 89r	Cassette 89 ORF rev	TGAGACATTACGCAGTTAAA	Method validation
VhC16f	Cassette 16 ORF fwd	AAAAGTCGCTCAGAAGAATA	Promoter isolation
VhC16r	Cassette 16 ORF rev	GCATTACGGATACTTGTCTT	Promoter isolation
VhC17f	Cassette 17 ORF fwd	ACTGGTCAAAATACAACCAT	Promoter isolation
VhC17r	Cassette 17 ORF rev	TACAACATCGAGCTAACAAA	Promoter isolation
VhC18f	Cassette 18 ORF fwd	GGTTTGATAGTTACGCTGAT	Promoter isolation
VhC18r	Cassette 18 ORF rev	CAACCAAATGTGATAATGAA	Promoter isolation
VhC19f	Cassette 19 ORF fwd	GAGTGCAGCAGGTTATTTAT	Promoter isolation
VhC19r	Cassette 19 ORF rev	ATACTGACCGATAACTTTGG	Promoter isolation

ISSN: 2730-6844