values between TS and WS white-throated sparrow groups across the genome. The ZAL2/2m data point represents FST within the inversion interval between the arrangements and is the only value to show significant population structure (p < 0.01). The ZAL2/2m† data point represents the FST outside the inversion on white-throated sparrow chromosome 2. Note that the chromosomes labeled as Chr refer to the predicted orthologous chromosome in chicken. Also note the negative FST values are an artifact of the method used to calculate this statistic using sequence comparisons and are not significantly different from 0.