Table 8 Primer combinations for amplifying the complete mitochondrial sequences
From: Coexistence of diploid, triploid and tetraploid crucian carp (Carassius auratus) in natural waters
Primers | Nucleotide sequence (5'→3') | Primers | Nucleotide sequence (5'→3') |
|---|---|---|---|
cytb-F1 | AATGACTTGAAGAACCACCGT | cytb-F2-2 | TTCTTTCCACCCATACTTT |
cytb-R1 | CTCCGATCTTCGGATTACAAGAC | cytb-R2-2 | AGGAACCAGATGCCAGTA |
CR-F | ACCCCTGGCTCCCAAAGC | 12SrRNA-F | ACAAAGCATAGCACTGAAGATG |
CR-R | ATCTTAGCATCTTCAGTG | 12SrRNA-R | TTTGCATGGATGTCTTCTCG |
12SrRNA-R-R1 | GAAACAGTGCTTGAAGGAGG | 16SrRNA-F2 | CGCCTGTTTACCAAAAACATCG |
16SrRNA-F2-R1 | AGCCCTCGTTTAGCCATT | 16SrRNA-R2 | CCGGTCTGAACTCAGATCA |
ND1-F | TCGACGAGGGGGTTTACGAC | ND2-2F | TCCTGGTGCTTCCTTTAC |
ND1-R | AGTAGTTCCTAGTCCTAGGC | ND2-2R | AGCGGTTCCTACTATTCC |
ND2-2R-F | GGCCTCGATCCTACAAAC | COI-F2 | TCCTCCTTCTCCTATCACT |
COI-F2-F | CGTCCATTCCGACAGTAA | COI-R2 | CTGGGACTGCGTCTATTT |
COII-F | AATTGCTCTACCATCCCT | ATPase-F | AAAGCGTTGGCCTTTTAAGC |
COII-R | GCTCATTTATGTCCTCCT | CR-F-F | AAGGAGGACCCAAGAACG |
CoNd3-2F | CTAAGCCTATACCTACAAGAA | ND4-2F | TTTCTTTACGCTTCTTCC |
CoNd3-2R | AACATAAGAGTGCGGAGA | ND4-2R | GCAAATTGACCCTGTTAT |
ND56-2F-2 | GCTACACTTATCCCAACC | ND56-2R-2-F | TGCCCTCTATGTAACCTGAT |
ND56-2R-2 | TAACCCGATGTCTCCTAC | ND56-4F-F | TTCCTCGTGAGATGTCGT |
ND56-4F | CTAGCCAACATGACGAGC | Â | Â |
ND56-4R | TGAGAATGCGGTTGAAAT | Â | Â |