Nucleotide sequence alignment of sa MSTN-2 promoter alleles (distal promoter). Sequences for saMSTN-2b and -2a alleles are consensus sequences of 10 different clones of various length at the 5' end, obtained from fish #17G genomic DNA. Partial sequence for saMSTN-2as is a consensus sequence of amplified fragment of two genomic DNAs, #8C and #13C, from the fifth DNA collection, which was obtained by PCR using the 'a'-specific forward primer MSTNb-11 and reverse primer MSTNb-1 and sequenced with primer MSTNb-11. Sequence for saMSTN-2c is the consensus sequence from two clones obtained from amplified genomic DNA of fish #13 from the Ardag collection using the 'c'-specific forward primer MSTNb-14 and reverse primer MSTNb-7, cloned in pGEM-Teasy vector and sequenced with primers T7/SP6, MSTNb-3 and MSTNb-1. The first 19 nucleotides of allele 'c' derived from the cloned fragment of genomic DNA of fish #0661 (sire of cross1) (see Materials and Methods). Sequences corresponding to primers are underlined with single black line and the names are indicated underneath. Repeated sequences are double-underlined in blue. Gaps (-) are indicated in the individual sequences. Sequences highlighted in color are non-identical sequences between a, b, c, and as alleles (yellow, pink, turquoise and green, respectively). Numbers on the left indicate distance from position +1 which has been assigned to the first nucleotide of the translation start codon ATG. GenBank Accession numbers of MSTN-2a, -2b, -2as and -2c are GQ379809, GQ379810, GQ379811, and HQ380026, respectively.