Sequence analysis of sa MSTN-2 gene. Comparison between saMSTN-2 gene and the published saMSTN-2 cDNA sequences. MSTN-2 (2R-17G) refers to the nucleotide sequence of saMSTN-2 gene based on 2R-17G allele. Deduced amino acid sequence is shown in single-letter code. Sequences corresponding to primers are underlined and the names are indicated underneath. Exons are in uppercase and introns in lowercase letters. Exon1: 328 bp; Intron1: 938 bp; Exon2: 371 bp; Intron2: 338 bp; Exon3: 381 bp (until stop codon TGA); +1 refers to the first nucleotide of the translation start codon. The two nucleotides that are different from the published sequence are highlighted. GenBank accession number for saMSTN-2 gene sequence (MSTN-2 variant 2R-17G) is GQ379805. AY046314, MSTN-2 cDNA sequence .