DNA sequence polymorphisms within the bovine guanine nucleotide-binding protein Gs subunit alpha (Gsα)-encoding (GNAS) genomic imprinting domain are associated with performance traits

Table 1 Summary statistics for the GNAS domain SNPs analysed in this study across 848 Holstein-Friesian sires

SNP	Open reading frame (ORF) model position of SNP	SNP location/dbSNP accession number	Genotypes	Genotype frequencies	Minor allele frequency	Deviations from HWE (P-value)
rs43101491	Upstream of the GNAS gene	58,215,520	GG	0.005	0.09 (G)	0.24
			GT	0.170
			TT	0.825
rs43101493	Upstream of the GNAS gene	58,214,963	AA	0.660	0.19 (G)	0.19
			AG	0.300
			GG	0.040
rs43101486	Upstream of the GNAS gene	58,203,762	AA	0.020	0.14 (A)	0.56
			AG	0.240
			GG	0.740
rs43101485	Upstream of the GNAS gene	58,203,464	CC	0.043	0.19 (C)	0.36
			CG	0.303
			GG	0.654
rs41694646	Intron 2 of the GNAS gene	58,183,623	CC	0.090	0.32 (C)	0.35
			CG	0.450
			GG	0.460
rs41694656	Intron 1 of the putative NESP55 gene	58,281,228	AA	0.747	0.14 (G)	0.25
			AG	0.229
			GG	0.024

Genotype frequency, minor allele frequency (MAF) and significance of deviation for Hardy-Weinberg equilibrium (HWE) based on P-values obtained from χ2-test results are given for all seven SNPs. dbSNP database accession numbers for each SNP were provided from GenBank [[53]; http://www.ncbi.nlm.nih.gov/projects/SNP] and the Build 4.0, release 60, of the B. taurus genome assembly in the ENSEMBL database http://www.ensembl.org.

ISSN: 2730-6844