Table 4 Work flow
From: Mapping of QTL affecting incidence of blood and meat inclusions in egg layers
Study | Aim | Material | Method | details |
|---|---|---|---|---|
Genome scan | Search for QTL | Sub data of F2 population (randomly selected 7 half-sib families) | Linkage analyses of 162 microsatellites on 27 chromosomes | 668 F2 hens |
Fine-mapping | Focusing on the QTL area | Whole F2 mapping population (17 half-sib families) | 6 new microsatellite markers + 5 SNPs | 1599 F2 hens |
Sequencing | SNP detection | Lohmann Brown, Hy-Line | Â | 106 indivuals in LB 20 invidivuals in Hy |
Histo-pathology | Tissue analysis | Eggs from a broiler breeding hatchery | Light microscopy study | 480 eggs |
Confirmation | Association studies in independent commercial lines | 1) Lohmann Brown (767 hens) | Mixed models with DMU | whole pure line hen population (767 hens): MCW241 -sub data I: ZO-2 (516 hens) -sub data II: phenotypically extreme 416 hens: panel of 15 SNPs |
| Â | Â | 2) Hy-Line (290 males) | Linear model with least squares, SAS | -all genotyped with MCW241 -all genotyped with a panel of 5 informative SNPs |