A second-generation anchored genetic linkage map of the tammar wallaby (Macropus eugenii)

BMC Genomic Data

Table 5 Linkage map end-markers used in genome coverage estimation, their BAC clones and overgo sequences, probe colours, cM and percentage coverage of the chromosome.

Chr	End marker	BAC clone	Overgo sequences	Probe colour (Fig. 1)	cM length covered by end markers FISH-mapped in Fig. 2	% of chr included	chr length (cM)	m%^c	n%^d
	KJW105	MeVIA_123J11	NA^a	Green	321.2	93.76	342.6	3.51	2.73
1	Y148	MeKba_448F6	a: CCAAACTGTAATGAAGGTTCAATG b: GCAGGTTACTTAGCTACATTGAAC	Red
2	MeVIA3N11-345	MeVIA_3N11	NA	Green	138.9	56.24	247.0	18.21	25.55
	MeKba206L23-25864	MeKba_206L23	NA	Red
3	DNHD2	MeKba_175F3	NA	Green	230.5	95.09	242.4	3.18	1.73
	G16-2	MeKba_323D15	a: GAGTTCAAATCCAGTCTCTCTTAC b: CAGGGTTGCATAGTGAGTAAGAGA	Red
4	MeVIA2J14-517	MeVIA_2J14	NA	Green	165.2	88.93	185.8	9.89	1.18
	PA55	MeKba_369C8	a: TCTACAAAATGAGGATAATACTCC b: GAACCCCTGTGAAGTAGGAGTATT	Red
5	PTCHD1	MeKba_555M23	NA	Green	200.2	86.04	232.7	1.89	12.16
	T31-1	MeKba_345M2	a: CTGGGATTCTAAGAGGGTGAGAAG b: TCCCCAAATCCTGGTTCTTCTCAC	Red
6	MeVIA2P1-275	MeVIA_2P1	NA	Green	129.6	96.58	134.2	2.72	0.69
	C2orf54	MeKba_307P14	Na	Red
7	G31-1	MeKba_80D8	a: TATTTCCCTAGGGAGGGACATCTG b: AGGAAGATGGTGCTTGCAGATGTC	Green	107.9	87.17	123.8	1.38	11.45
	KJW220	MeVIA_115N20	NA	Red
X	KJW281	MeVIA_15A6	NA	Green	88.1	25.84 (46.43^b)	189.7^b	70.36	3.81
	MeKba494M2-50126	MeKba_494M2	NA	Red
			TOTALS		1381.6		1698.2

The final column shows the estimated full chromosome lengths, calculated from the previous two columns.
^a NA = not available, as no overgo probes were designed for this marker.
^b The value 46.43 is the estimated % coverage of Xq and 189.7 is the estimated cM size of Xq, as the 25.84% physical size coverage of MEUX may not reflect the linkage length coverage because of a relative large nucleolus organiser region (NOR) on Xp.
^c m% = the percentage of the length of the p arm not covered by the linkage map, for a particular chromosome.
^d n% = the percentage of the length of the q arm not covered by the linkage map, for a particular chromosome.

ISSN: 2730-6844