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Table 2 Analysis of CEN5-GFP dots and spindle lengths in wild-type and chl1 cells treated with 0.2 M HU for 4 hours at 30°C

From: Functional characterization of the Saccharomyces cerevisiae protein Chl1 reveals the role of sister chromatid cohesion in the maintenance of spindle length during S-phase arrest

Strain % cells with
localized and
split dots
% cells with
localized and
unsplit dots
% cells with
other dots
(Y+G, G, G+G)
Average spindle length
Average distance between Y+Y dots
CHL1 16 78 5.4 1.04 ± 0.35 0.68 ± 0.23
chl1 37 44 19 1.50 ± 0.64 1.33 ± 0.71
  1. Cells from Figure 3C were analyzed for the localization of CEN5-GFP dots on the spindle, average spindle lengths and sister centromere separation. Y and Y+Y refer respectively to unsplit and split sister kinetochores on the spindle. G and G+G refer to unsplit and split sister kinetochores, respectively, not on the spindle. Y+G refers to split kinetochores in which one sister lies on and the other outside the spindle. 60-100 cells were analyzed in each case. The statistical significance of the spindle length comparisons was validated by the p-value of ≤ 0.001.