Table 3 List of primer sequences used for typing MUC4 polymorphisms and quantitative PCR
Application | SNP/Gene | Primer Sequence (5' →3') | Length (bp) |
|---|---|---|---|
Pyrosequencing PCR | DQ124298:g.243A>G | F: *tggtgctacccccagatttg | |
R: gttgtgtccaccccttacccttat | 195 | ||
P: gtcccctctcccaggta | |||
DQ124298:g.344A>G | F: *gtggccctcagtcactagagt | ||
R: cgaagttgtgaaaggaagacag | 258 | ||
P: ttggggttggggcag | |||
qPCR | MUC4 | F: atgggcttctccagtggagat | 66 |
R: tctcccacactggctgcaa |