MTH1 and RGT1 demonstrate combined haploinsufficiency in regulation of the hexose transporter genes in Saccharomyces cerevisiae
© Dietzel et al.; licensee BioMed Central Ltd. 2012
Received: 17 July 2012
Accepted: 4 December 2012
Published: 12 December 2012
The SNF3 gene in the yeast Saccharomyces cerevisiae encodes a low glucose sensor that regulates expression of an important subset of the hexose transporter (HXT) superfamily. Null mutations of snf3 result in a defect in growth on low glucose concentrations due to the inability to relieve repression of a subset of the HXT genes. The snf3 null mutation phenotype is suppressed by the loss of either one of the downstream co-repressor proteins Rgt1p or Mth1p. The relief of repression allows expression of HXT transporter proteins, the resumption of glucose uptake and therefore of growth in the absence of a functional Snf3 sensor.
Strains heterozygous for both the RGT1 and MTH1 genes (RGT1/rgt1Δ MTH1/mth1Δ snf3Δ/snf3Δ) but homozygous for the snf3∆ were found to grow on low glucose. Since null alleles in the heterozygous state lead to suppression, MTH1 and RGT1 display the phenomenon of combined haploinsufficiency. This observed haploinsufficiency is consistent with the finding of repressor titration as a mechanism of suppression of snf3. Mutants of the STD1 homolog of MTH1 did not display haploinsufficiency singly or in combination with mutations in RGT1. HXT gene reporter fusion assays indicated that the presence of heterozygosity at the MTH1 and RGT1 alleles leads to increased expression of the HXT2 gene. Deletion of the HXT2 gene in a heterozygous diploid, RGT1/rgt1Δ MTH1/mth1Δ snf3Δ/snf3Δ hxt2Δ/hxt2Δ, prevented the suppression of snf3Δ.
These findings support the model of relief of repression as the mechanism of restoration of growth on low glucose concentrations in the absence of functional Snf3p. Further, the observation that HXT2 is the gene responsible for restoration of growth under these conditions suggests that the numbers of repressor binding domains found in the regulatory regions of members of the HXT family may have biological relevance and enable differential regulation.
Glucose is the preferred carbon source for many organisms including the budding yeast Saccharomyces cerevisiae. Glucose transport occurs via facilitated diffusion mediated by a group of homologous transmembrane hexose transporters, encoded by the HXT genes . Transport is the rate limiting step in glucose utilization by S. cerevisiae and a complex regulatory network has evolved to maintain optimal transporter activity in response to external nutrient availability [1, 2]. Many signaling pathways are known to converge at the promoters of the HXT genes to either positively or negatively regulate specific transporters in response to their respective inputs. These pathways include: glucose induction, glucose repression, High Osmolarity Glycerol (HOG), Target of Rapamycin (TOR) GCR1/GCR2 pathway and the RAS/PKA pathway [3–8].
Although interpathway crosstalk is likely crucial to optimized HXT regulation, it is clear that the relief of HXT repression via the SNF3/RGT2 glucose induction pathway is the major on/off switch essential to transporter induction. Mutations in several components of this pathway show extreme growth defects on glucose that result from the inability to induce high level expression of the major HXT genes [9–11]. Snf3p and Rgt2p, which display homology to the HXT transporters, are thought to act as sensors of low and high glucose respectively [12–14]. In contrast to the HXT's, both Snf3p and Rgt2p appear to have lost the capacity to transport glucose and both proteins also contain an extra C terminal cytoplasmic domain that is important for the transmission of the signal . Null alleles of snf3 result in the loss of fermentative growth on low levels of glucose (0.05%) while null alleles of both sensors result in the loss of fermentative growth on 2% glucose [11, 15, 16].
Mth1p and Std1p are the only known link between the cytoplasmic membrane bound sensors and the DNA binding repressor Rgt1p in the nucleus where they act to facilitate the repressor function of Rgt1p [11, 17, 18]. Rgt1p appears to regulate a limited set of genes including most of the HXT genes that are critical for growth on glucose [12, 13, 19]. It is not known how glucose specifically activates the sensors, but activation requires the cytoplasmic domains which are thought to facilitate the phosphorylation of the homologous corepressor proteins Mth1p and Std1p by the Yeast Casein Kinase (YCK1, YCK2) [11, 14, 16, 20]. Phosphorylation targets the corepressors for ubiquitination which results in their degradation . Degradation of Mth1p and Std1p is thought to inactivate the repressor function of Rgt1p and allow for its phosphorylation by the Protein kinase A homologues [7, 8, 17, 19]. As a result, the promoters of the HXT genes are then open for transcription.
Although a set of key proteins have been identified and a working model of the glucose induction pathway exists, many questions remain about how these components interact and function to regulate HXT gene expression in response to external glucose levels. During complementation analyses of repressor mutants it was discovered that diploid strains homozygous for the snf3 null mutation and heterozygous at both the MTH1 and RGT1 loci (snf3Δ/snf3Δ MTH1/mth1 RGT1/rgt1) were able to grow on low glucose. Haploinsufficiency arises when a reduction in copy number results in an observable phenotype . The appearance of a phenotype in the presence of recessive alleles of different genes has been termed combined haploinsufficiency . Combined haploinsufficiency arises when reduction in dosage of one gene displays a mutant phenotype only in the presence of an accompanying reduction in dosage of a second gene. This genetic phenomenon is rare and typically implies that the proteins involved form a complex and that loss of a critical concentration of the complex is responsible for the observed phenotype. This observation indicates that the levels of both of these proteins are in a critical balance with the number of promoters that they regulate. The impacts of this finding on the current model of the glucose induction pathway are discussed.
Results and discussion
MTH1 and RGT1 display combined haploinsufficiency
The MTH1 gene encodes a co-repressor protein that is able to bind to the repressor RGT1 stabilizing binding of the complex to the regulatory regions of the HXT genes. In the presence of a glucose signal mediated by either Snf3p or Rgt2p Mth1p is phosphorylated and targeted for ubiquitination and Rgt1-mediated repression is inactivated. Deletion of either MTH1 or RGT1 restores expression of the HXT genes as the repressor complex cannot be established. Loss of either of these genes is recessive to the wild type, indicating that in a diploid situation only a single functioning repressor gene is necessary.
In the course of analysis of missense mutations in rgt1 and mth1 we observed suppression of the snf3Δ growth defect on low glucose in strains that were heterozygous at these loci (snf3Δ/snf3Δ RGT1/rgt1 MTH1/mth1). This finding suggested one of two possibilities for the mechanism of suppression: non-allelic non complementation or combined haploinsufficiency. In non-allelic non complementation the appearance of a phenotype is due to the creation of an aberrant regulatory complex that in this case would presumably be leading to activation of expression. On the other hand, combined haploinsufficiency occurs when the decrease in gene dosage of two genes with interacting gene products results in a dynamic reduction in the level of the complex itself and expression of the mutant phenotype. Suppression in this case would be mediated by relief of repression due to the decrease in repressor complex availability. Combined haploinsufficiency is a rare genetic phenomenon that implies physical interaction of the gene products . These two phenomena can be distinguished by investigation of the phenotype of null mutations. If suppression still exists in the presence of heterozygous null mutations then loss of repressor complex rather than formation of an aberrant regulatory complex is the mechanism by which growth is restored to snf3Δ strains.
To test this possibility, complete null alleles were created of both mth1 and rgt1 in the snf3Δ background (UCD2875 and UCD2876, respectively). Both mutations independently suppressed the snf3Δ growth defect in haploid strains as expected and both acted as fully recessive mutations in homozygous diploids also as expected. However heterozygous diploids at both of these loci (RGT1/rgt1Δ snf3Δ/snf3Δ and MTH1/mthΔ snf3Δ/snf3Δ) (UCD2881) failed to complement and growth on low glucose occurred indicating that combined haploinsufficiency is the genetic phenomenon underlying apparent suppression of the snf3Δ phenotype.
Loss of the MTH1 homolog STD1 does not suppress loss ofSNF3
STD1 has been reported to be somewhat functionally redundant to MTH1 and to be transcriptionally regulated by the RGT1 repressor complex similar to the HXT genes [19, 23]. This “feed forward” regulation has been proposed to act to quickly restore the repressor complex as glucose levels drop . Therefore the role of STD1 in suppression of the snf3 growth defect was evaluated.
Thus STD1 did not show combined haploinsufficiency in the presence of heterozygosity at the RGT1 locus. Due to the significant amount of sequence identity (61%) and somewhat overlapping yeast two hybrid interactions with the tail domains of the glucose sensors and also with Rgt1p, the corepressor proteins Mth1p and Std1p have been described in a manner that implies that they are functionally redundant or at least have overlapping functions [23, 24]. In contrast, other studies have concluded that Mth1p is likely to be more specific to the regulation of HXT genes than Std1p [11, 16, 17] but agree that some data suggests enhanced derepression when both genes are deleted. Our analysis suggests that Mth1p alone is primarily responsible for maintaining repression downstream of Snf3p when glucose levels are limiting. In addition, our finding that deleting std1 along with mth1 does not augment adaptation to low glucose and that the deletion of std1 shows no detectable effect on the adaptation of snf3 null strains to low glucose lead us to conclude that Std1 has little effect in regulating Rgt1p function under the low glucose conditions used in this study. However, our observation that the overexpression of STD1 from a strong promoter prevents the suppression of the snf3 growth defect by an mth1 null mutant and that this requires a functional Rgt1p corroborates the conclusion that Std1 can act to prevent growth on low glucose, but implies that it may not normally do so. The discovery that the [GAR+] prion allows growth on alternate substrates in the presence of glucose due to a specific interaction of the Pma1p and Std1p proteins fostering the creation of a stable Std1p-Rgt1p complex  strongly supports the idea that Std1p regulates HXT gene expression under specific physiological conditions.
Low glucose growth curves confirm combined and single haploinsufficiency
While the loss of snf3 function results in the inability to grow on low glucose solid media, it results in a dramatic increase in the time it takes to adapt to low glucose liquid media and a diminished capacity to achieve the same final OD of wild type strains . The spot plate assays suggested that there may be differences in adaptation times due to the single and combined haploinsufficiency observations.
The combined haploinsufficiency phenotype is a result of HXT gene expression
Because Mth1p and Rgt1p are known to regulate the expression of many of the HXT genes, β-galactosidase reporter assays were used to examine the expression of the HXT genes most likely to be relevant to adapting to low glucose conditions. Reporter plasmids with the regions spanning at least −950 up to the start codon of HXT2, HXT3 and HXT4 were transformed into diploid strains with different combinations of mutations of components of the pathway. These strains were grown under fully repressing conditions on galactose, shifted to low glucose plus Antimycin A and then harvested and assayed for β-galactosidase activity.
Yeast strains used in this study
MATa snf3Δ4::HIS3 mth1Δ::HphMX
MATa snf3Δ4::HIS3 rgt1Δ::KanMX
MATa snf3Δ4::HIS3 std1Δ::KanMX
MATα snf3Δ::TRP1 std1Δ::KanMX
MATa/MATα snf3Δ4::HIS3/snf3Δ::TRP1 mth1Δ::HphMX/MTH1
MATa/MATα snf3Δ4::HIS3/snf3Δ::TRP1 rgt1Δ::KanMX/RGT1
MATa/MATα snf3Δ4::HIS3/snf3Δ::TRP1 mth1Δ::HphMX/MTH1 rgt1Δ::KanMX/RGT1
MATa/MATα snf3Δ4::HIS3/snf3Δ::TRP1 mth1Δ::HphMX/mth1Δ::HphMX
MATa/MATα snf3Δ4::HIS3/snf3Δ::TRP1 rgt1Δ::KanMX/rgt1Δ::KanMX
MATa/MATα snf3Δ4::HIS3/snf3Δ::TRP1 mth1Δ::HphMX/mth1Δ::HphMX rgt1Δ::KanMX/rgt1Δ::KanMX
MATa/MATα snf3Δ4::HIS3/snf3Δ::TRP1 std1Δ::KanMX/std1Δ::KanMX
MATa/MATα snf3Δ4::HIS3/snf3Δ::TRP1 mth1Δ::HphMX/mth1Δ::HphMX std1Δ::KanMX/std1Δ::KanMX
MATα snf3Δ::TRP1 hxt2∆ ::KanMX
MATa snf3Δ::TRP1 hxt2∆ ::KanMX
MATa snf3Δ::TRP1 hxt2∆ ::KanMX rgt1:HphMX
MATα snf3Δ::TRP1 hxt2∆ ::KanMX mth1::HphMX
MATα snf3Δ::TRP1/ snf3∆ ::TRP1 hxt2∆ ::KanMX/ hxt2∆ ::KanMX rgt1:HphMX/RGT1 mth1::HphMX/MTH1
β-galactosidase activity of HXT 2, 3 and 4 promoter fusions to LacZ
LacZ Fusion Reporter Assay*
β-Galactosidase Activity (nmole/min/mg protein)
snf3/snf3 rgt1/RGT1 mth1/MTH1
β-galactosidase activity expressed from the HXT2 promoter fusions of strains following shift to low glucose or galactose
LacZFusion Reporter Assay*
β-Galactosidase Activity (nmole/min/mg protein)
snf3/snf3 mth1/MTH1 rgt1/RGT1
snf3/snf3 mth1/mth1 rgt1/rgt1
Specific derepression of HXT2 is responsible for growth of the heterozygotes
Analysis of the distribution of Rgt1 binding sites in HXT gene promoter regions revealed a direct connection between the number of binding sites and the level of repression. Addition of binding sites to a reporter gene construct increased repression 10 to 50 fold . These authors speculated that Rgt1-mediated repression of genes containing less than five Rgt1-binding sites likely required the presence of another repressor. The HXT2 promoter was shown to carry only three Rgt1 binding sites while the promoters of the HXT1 and HXT3 genes carried 13 and 15 putative binding sites for Rgt1p-mediated repression respectively . Our findings suggest that the combination of both Rgt1 and Mth1 as co-repressors is required for full HXT2 repression. When considered in combination with the previous reports that 2 micron plasmids containing only the promoters of genes regulated by this complex can suppress the snf3 growth defect , the clear conclusion is that this pathway has evolved a fine balance between the number of promoter binding domains and repressor complex availability. This balance allows for effective gene repression when glucose is not present yet efficient and prompt derepression when glucose is available.
These observations also suggest that repressor binding site number may represent yet another mechanism to differentially regulate the different hexose transporters. The Hxt2 transporter has been shown to exist in forms with differing affinity for substrate  although under some conditions it appears to be a simple high affinity transporter . Hxt2p has also been shown to be expressed early in wine fermentation when sugar substrates are in high concentration, but then is quickly degraded [30–32]. These observations suggest that the HXT2 protein may be an important transporter for the transition between substrate concentrations and that the affinity of the transporter may be impacted by other factors within the cell. It is also possible that under very high substrate conditions the Hxt2 transporter plays an important regulatory role and that putative regulatory role rather than level of transporter protein expression is the key factor enabling growth of the heterozygous strain. A rapid induction of HXT2 may therefore confer a distinct growth advantage to the cells during adaptation to differing substrate concentrations. Differences in numbers of repressor binding sites could play a role in allowing expression of some transporters to occur under otherwise limiting conditions.
This information adds unique insight to the model that has formed over the past decade since the roles of the corepressor proteins were first elucidated and this intriguing mechanism of transcriptional regulation began to be understood [11, 16, 17]. One of the most striking features of this pathway is the apparent large expense of cellular energy required to utilize a signaling system based on the complete degradation of the corepressor proteins Mth1p and Std1p. The observation of combined haploinsufficiency indicates that one mechanism by which this system conserves energy is that just enough corepressor protein is available to maintain repression of the target genes of the pathway. As a result, the system is extremely sensitive and responsive to subtle changes in corepressor numbers. Keeping the level of corepressor protein to the minimum amount necessary to maintain repression allows for quick induction of gene expression when the preferred carbohydrate returns.
Yeast strains, genetic techniques, and growth media
The strains of Saccharomyces cerevisiae used in this study are listed in Table 1 above. The MATa deletion set of the Saccharomyces Genome Deletion Project was purchased from Open Biosystems and used to make all complete null alleles that contain the KanMX resistance marker that provides resistance to G418. Genomic DNA was purified by using the MasterPure™ Yeast DNA purification kit (Epicentre). Genomic DNA of the respective null strain from the deletion set was used as the template for PCR mediated gene disruption by amplifying approximately 200 base pairs of homology flanking each side of the deleted ORF. For all deletions that contain the HphMX resistance marker specifying resistance to Hygromycin B, the pYC140 plasmid  was used as a template for PCR with primers with at least 40 base pairs of flanking homology just outside of the open reading frame. The linear PCR products were PCR purified (Qiagen) and transformed by the method of Gietz and Woods and selected for with the appropriate antibiotic . All deletions were made in haploid strains and confirmed by colony PCR as described in . Diploids were made by mating the appropriate haploid strains. Standard genetic techniques were used for mating and sporulation.
Yeast strains were grown on Yeast Extract Peptone Media (YP) consisting of 1% yeast extract, 2% Bacto peptone and either 2% glucose, 2% galactose, or 0.05% glucose. Synthetic complete (SC) or synthetic dropout (SD) media were made according to established protocols  with the appropriate amino acid or nitrogen base omitted. Bacto agar at 2% w/v was used for solid media. Antimycin A was added at 1 μg/mL to low glucose (0.05%) media. When needed for selection, G418 was used at 200 μg/ml or Hygromycin B used at 300 μg/mL. Growth on low glucose was assessed by streaking strains to YP 0.05% glucose plus Antimycin A or SD with the necessary dropout to maintain selection for the plasmid plus 0.05% glucose and Antimycin A. Growth was assessed after 3 days of incubation at 30°C.
Construction of overexpression vector for STD1
The complete open reading frame of STD1 was cloned into the p416TEF vector which contains the URA3 selectable marker  by using PCR primers that introduced 5’ EcoRI and 3’XhoI flanking sites. The respective sites were then used to clone into the vector and the open reading frame was sequenced from the vector to ensure the fidelity of the amplification and cloning. The resulting vector, pKD-TEF-STD1 contains the complete open reading frame of STD1 preceded by the strong constitutive TEF promoter and flanked by the CYC1 terminator sequence.
The LacZ reporter fusions to the promoters of HXT2, HXT3, and HXT4 were made in the CEN/ARS vector pRS416 which contains the URA3 selectable marker . The promoter regions spanning at least −950 up to the start codon of HXT2, HXT3 and HXT4 were PCR amplified with 5’ SacI and 3’NotI overhangs and cloned into pRS416 to create pKD-HXT2, pKD-HXT3 and pKD-HXT4. The resulting plasmids were then sequenced to ensure the fidelity of the cloning. The E. coli LacZ gene from the one hybrid reporter vector described in  was amplified by PCR with primers that introduced 5’ NotI and 3’ XmaI overhangs and cloned into the respective sites of pKD-HXT2, pKD-HXT3 and pKD-HXT4 to make pKD-HXT2 LacZ, pKD-HXT3 LacZ and pKD-HXT4LacZ. The resulting plasmids contain at least 950 base pairs of the promoter region up to the start codon and are in frame with the E. coli LacZ gene. All plasmids were transformed into the respective yeast strains as described in .
Reintroduction of HXT2 on a plasmid
A plasmid carrying a copy of HXT2 from YPH500 was constructed in the CEN/ARS vector pRS316, which contains the URA3 yeast selectable marker . The HXT2 gene, along with 562bp of the upstream region, was PCR amplified using HiFi Taq (Invitrogen #11304-011) with primers containing 5’ XhoI (5’-CTCGAGTTTCCGTGAAATAGATTC-3’) and 3’ XbaI (5’-TCTAGATATTAGTAGCCATTAGCC-3’) overhangs. The resulting PCR product was cloned into the MCS of pRS316. The plasmid, now carrying a functional copy of HXT2 under its native promoter, was transformed into previously hxt2 null strains .
Low glucose growth curves
Strains were streaked out from glycerol stocks to YPD media and a separate single colony was used to inoculate three replicate starter cultures in Synthetic Complete (SC) 2% glucose media. Starter cultures were grown for 48 hours and used to inoculate 200 mL of SC 0.05% glucose plus Antimycin A to an OD580 of 0.02. Cultures were grown in 500 mL non baffled Erlenmeyer flasks at 30°C with shaking on an orbital shaker at 200 RPM and the OD monitored at 580 nm. All strains were examined in triplicate and each figure represents one experiment where all strains shown were grown at the same time. Error bars indicate the standard deviation of the average of the three triplicates.
Spot plate assays
Overnight cultures of strains were grown in YP 2% glucose, washed once with sterile water and then re-suspended in sterile water to an OD580 of 1.0. The strains were then subjected to a 10 fold serial dilution and 10 μL of each was spotted on YP or SC plus 0.05% glucose and Antimycin A. Growth was assessed after 3 days of incubation at 30°C.
LacZ reporter assays
Strains with reporter plasmids were grown to mid log phase in SD minus uracil with 2% galactose, harvested by centrifugation and then resuspended in either SD minus uracil with 0.05% and Antimycin A or SD minus uracil with 2% galactose and allowed to grow for 3 hours in the respective media. Approximately 15 OD units were harvested by centrifugation and resuspended in assay buffer and assayed as described in . β-galactosidase units were measured as nmoles of 2-nitrophenyl-β-galactopyranoside released per minute per mg of total protein as determined by Bradford Assay (Biorad). All assays were performed in triplicate and comparisons made between strains that were cultured and assayed at the same time. T-test (LSD) statistical analyses of the LacZ data were performed using SAS (SAS Institute Inc., Cary, NC).
KLD current address: Amyris Biotechnologies, 5885 Hollis Street, Suite 100, Emeryville, CA
This research was supported by the Maynard A. Amerine endowment.
- Bisson LF, Coons DM, Kruckeberg AL, Lewis DA: Yeast sugar transporters. Crit Rev Biochem Mol Biol. 1993, 28: 259-308. 10.3109/10409239309078437.View ArticlePubMedGoogle Scholar
- Ozcan SJ: Function and regulation of yeast hexose transporters. Microbiol Mol Biol Rev. 1999, 63: 554-569.PubMed CentralPubMedGoogle Scholar
- Wendell DL, Bisson LF: Expression of high-affinity glucose transport protein Hxt2p of Saccharomyces cerevisiae is both repressed and induced by glucose and appears to be regulated posttranslationally. J Bacteriol. 1994, 176: 3730-3737.PubMed CentralPubMedGoogle Scholar
- Ozcan S, Johnston M: Three different regulatory mechanisms enable yeast hexose transporter (HXT) genes to be induced by different levels of glucose. Mol Cell Biol. 1995, 15: 1564-1572.PubMed CentralView ArticlePubMedGoogle Scholar
- Tomas-Cobos L, Viana R, Sanz P: TOR kinase pathway and 14-3-3 proteins regulate glucose-induced expression of HXT1, a yeast low-affinity glucose transporter. Yeast. 2005, 22: 471-479. 10.1002/yea.1224.View ArticlePubMedGoogle Scholar
- Tomas-Cobos L, Casadome L, Mas G, Sanz P, Posas F: Expression of the HXT1 low affinity glucose transporter requires the coordinated activities of the HOG and glucose signaling pathways. J Biol Chem. 2004, 279: 22010-22019. 10.1074/jbc.M400609200.View ArticlePubMedGoogle Scholar
- Kim JH, Johnston M: Two glucose-sensing pathways converge on Rgt1 to regulate expression of glucose transporter genes in Saccharomyces cerevisiae. J Biol Chem. 2006, 281: 26144-26149. 10.1074/jbc.M603636200.View ArticlePubMedGoogle Scholar
- Palomino A, Herrero P, Moreno F: Tpk3 and Snf1 protein kinases regulate Rgt1 association with Saccharomyces cerevisiae HXK2 promoter. Nucleic Acids Res. 2006, 34: 1427-1438. 10.1093/nar/gkl028.PubMed CentralView ArticlePubMedGoogle Scholar
- Ozcan S, Freidel K, Leuker A, Ciriacy M: Glucose uptake and catabolite repression in dominant HTR1 mutants of Saccharomyces cerevisiae. J Bacteriol. 1993, 175: 5520-5528.PubMed CentralPubMedGoogle Scholar
- Vallier LG, Coons D, Bisson LF, Carlson M: Altered regulatory responses to glucose are associated with a glucose transport defect in grr1 mutants of Saccharomyces cerevisiae. Genetics. 1994, 136: 1279-1285.PubMed CentralPubMedGoogle Scholar
- Schmidt MC, McCartney RR, Zhang X, Tillman TS, Solimeo H, Wölfl S, Almonte C, Watkins S: Std1 and Mth1 proteins interact with the glucose sensors to control glucose-regulated gene expression in Saccharomyces cerevisiae. Mol Cell Biol. 1999, 19: 4561-4571.PubMed CentralView ArticlePubMedGoogle Scholar
- Ozcan S, Dover J, Rosenwald AG, Wolfl S, Johnston M: Two glucose transporters in Saccharomyces cerevisiae are glucose sensors that generate a signal for induction of gene expression. Proc Natl Acad Sci USA. 1996, 93: 12428-12432. 10.1073/pnas.93.22.12428.PubMed CentralView ArticlePubMedGoogle Scholar
- Ozcan S, Leong T, Johnston M: Rgt1p of Saccharomyces cerevisiae, a key regulator of glucose-induced genes, is both an activator and repressor of transcription. Mol Cell Biol. 1996, 16: 6419-6426.PubMed CentralView ArticlePubMedGoogle Scholar
- Coons DM, Vagnoli P, Bisson LF: The C-terminal domain of Snf3p is sufficient to complement the growth defect of snf3 null mutations in Saccharomyces cerevisiae: SNF3 functions in glucose recognition. Yeast. 1997, 13: 9-20. 10.1002/(SICI)1097-0061(199701)13:1<9::AID-YEA51>3.0.CO;2-U.View ArticlePubMedGoogle Scholar
- Bisson LF, Neigeborn L, Carlson M, Fraenkel DG: The SNF3 gene is required for high-affinity glucose transport in Saccharomyces cerevisiae. J Bacteriol. 1987, 169: 1656-1662.PubMed CentralPubMedGoogle Scholar
- Lafuente MJ, Gancedo C, Jauniaux JC, Gancedo JM: Mth1 receives the signal given by the glucose sensors Snf3 and Rgt2 in Saccharomyces cerevisiae. Mol Microbiol. 2000, 35: 161-172. 10.1046/j.1365-2958.2000.01688.x.View ArticlePubMedGoogle Scholar
- Flick KM, Spielewoy N, Kalashnikova TI, Guaderrama M, Zhu Q, Chang HC, Wittenberg C: Grr1-dependent inactivation of Mth1 mediates glucose-induced dissociation of Rgt1 from HXT gene promoters. Mol Biol Cell. 2003, 14: 3230-3241. 10.1091/mbc.E03-03-0135.PubMed CentralView ArticlePubMedGoogle Scholar
- Polish JA, Kim JH, Johnston M: How the Rgt1 transcription factor of Saccharomyces cerevisiae is regulated by glucose. Genetics. 2005, 169: 583-594. 10.1534/genetics.104.034512.PubMed CentralView ArticlePubMedGoogle Scholar
- Kaniak A, Xue Z, Macool D, Kim JH, Johnston M: Regulatory network connecting two glucose signal transduction pathways in Saccharomyces cerevisiae. Eukaryot Cell. 2004, 3: 221-231. 10.1128/EC.3.1.221-231.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Moriya H, Johnston M: Glucose sensing and signaling in Saccharomyces cerevisiae through the Rgt2 glucose sensor and casein kinase I. Proc Natl Acad Sci U S A. 2004, 101: 1572-1577. 10.1073/pnas.0305901101.PubMed CentralView ArticlePubMedGoogle Scholar
- Deutschbauer AM, Jaramillo DF, Proctor M, Kumm J, Hillenmeyer ME, Davis RW, Nislow C, Giaver G: Mechanisms of haploinsufficiency revealed by genome-wide profiling in yeast. Genetics. 2005, 169: 1915-1925. 10.1534/genetics.104.036871.PubMed CentralView ArticlePubMedGoogle Scholar
- Hawley RS, Gilliland WD: Sometimes the result is not the answer: the truths and the lies that come from using the complementation test. Genetics. 2006, 174: 5-15. 10.1534/genetics.106.064550.PubMed CentralView ArticlePubMedGoogle Scholar
- Johnston M, Kim JH: Glucose as a hormone: receptor-mediated glucose sensing in the yeast Saccharomyces cerevisiae. Biochem Soc Trans. 2005, 33: 247-252. 10.1042/BST0330247.View ArticlePubMedGoogle Scholar
- Lakshmanan J, Mosley AL, Ozcan S: Repression of transcription by Rgt1 in the absence of glucose requires Std1 and Mth1. Curr Genet. 2003, 44: 19-25. 10.1007/s00294-003-0423-2.View ArticlePubMedGoogle Scholar
- Brown JC, Lindquist S: A heritable switch in carbon source utilization driven by an unusual yeast prion. Genes Dev. 2009, 23: 2320-2332. 10.1101/gad.1839109.PubMed CentralView ArticlePubMedGoogle Scholar
- Kim JH, Polish J, Johnston M: Specificity and regulation of DNA binding by the yeast glucose transporter gene repressor Rgt1. Mol Cell Biol. 2003, 23: 5208-5216. 10.1128/MCB.23.15.5208-5216.2003.PubMed CentralView ArticlePubMedGoogle Scholar
- Theodoris G, Bisson LF: DDSE: downstream targets of the SNF3 signal transduction pathway. FEMS Microbiol Lett. 2001, 197: 73-77. 10.1111/j.1574-6968.2001.tb10585.x.View ArticlePubMedGoogle Scholar
- Reifenberger E, Boles E, Ciriacy M: Kinetic characterization of individual hexose transporters of Saccharomyces cerevisiae and their relation to the triggering of mechanisms of glucose repression. Eur J Biochem. 1997, 245: 324-333. 10.1111/j.1432-1033.1997.00324.x.View ArticlePubMedGoogle Scholar
- Maier A, Volker B, Boles E, Fuhrmann GF: Characterization of glucose transport in Saccharomyces cerevisiae with plasma membrane vesicles (countertransport) and intact cells with single Hxt1, Hxt2, Hxt3, Hxt4, Hxt6, Hxt7 or Gal2 transporters. FEMS Yeast Res. 2002, 2: 539-550.PubMedGoogle Scholar
- Karpel JE, Place WR, Bisson LF: Analysis of the major hexose transporter genes in wine strains of Saccharomyces cerevisiae. Am J Enol Vitic. 2008, 59: 265-275.Google Scholar
- Luyten K, Riou C, Blondin B: The hexose transporters of Saccharomyces cerevisiae play different roles during enological fermentation. Yeast. 2002, 19: 713-726. 10.1002/yea.869.View ArticlePubMedGoogle Scholar
- Perez M, Luyten K, Michel R, Riou C, Blondin D: Analysis of Saccharomyces cerevisiae hexose carrier expression during wine fermentation: both low and high affinity Hxt transporters are expressed. FEMS Yeast Res. 2005, 5: 351-361. 10.1016/j.femsyr.2004.09.005.View ArticlePubMedGoogle Scholar
- Hansen J, Felding T, Johannesen PF, Piskur J, Christensen CL, Olesen K: Further development of the cassette-based pYC plasmid system by incorporation of the dominant hph, nat and AUR1-C gene markers and the lacZ reporter system. FEMS Yeast Res. 2003, 4: 323-327. 10.1016/S1567-1356(03)00178-8.View ArticlePubMedGoogle Scholar
- Gietz RD, Woods RA: Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Methods Enzymol. 2002, 350: 87-96.View ArticlePubMedGoogle Scholar
- Amberg DC, Burke DJ, Strathern JN: Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual. 2005, Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
- Mumberg D, Muller R, Funk M: Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene. 1995, 156: 119-122. 10.1016/0378-1119(95)00037-7.View ArticlePubMedGoogle Scholar
- Sikorski RS, Hieter P: A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 1989, 122: 19-27.PubMed CentralPubMedGoogle Scholar
- Ramakrishnan V, Theodoris G, Bisson LF: Loss of IRA2 suppresses the growth defect on low glucose caused by the snf3 mutation in Saccharomyces cerevisiae. FEMS Yeast Res. 2007, 7: 67-77. 10.1111/j.1567-1364.2006.00159.x.View ArticlePubMedGoogle Scholar
- Rupp S: LacZ assays in yeast. Methods Enzymol. 2002, 350: 112-131.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.