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Table 3 β-galactosidase activity expressed from the HXT2 promoter fusions of strains following shift to low glucose or galactose

From: MTH1 and RGT1 demonstrate combined haploinsufficiency in regulation of the hexose transporter genes in Saccharomyces cerevisiae

Relevant Genotype

                LacZFusion Reporter Assay*

 

                β-Galactosidase Activity (nmole/min/mg protein)

 

Glucose Shift

Galactose Shift

Wild Type

196.7±4.6b

21.5±2.7c,a

snf3/snf3

1.2±0.3c,c

1.6±0.3c,d

snf3/snf3 mth1/MTH1 rgt1/RGT1

9.2±1.0c,a

8.8±0.3c,b

snf3/snf3 rgt1/RGT1

4.2±0.7c,b

3.4±0.23c,c

snf3/snf3 mth1/MTH1

1.2±0.1c,c

1.1±0.41c,d

snf3/snf3 std1/std1

1.8±1.2c,c

1.4±0.67c,d

snf3/snf3 mth1/mth1

678±158.5a

589.8±49.6a

snf3/snf3 rgt1/rgt1

309.8±25.9b

321.5±12.98b

snf3/snf3 mth1/mth1 rgt1/rgt1

563.6±167.7a

574.9±113.5a

  1. *Strains were grown to mid log phase in SC-URA + 2% galactose and then transferred to either SC-URA with 2% galactose or to SC-URA with 0.05% glucose plus Antimycin A and harvested and assayed 3 hours after the shift. Values and standard deviation and represent the average of at least three transformants cultured separately with the same reporter construct. Different letters denote statistical significance at p < 0.05. The first set of letters represents differences among values of the entire data set. The second set of letters denotes statistical significance among activity values at or below 20 units.