Figure 3
From: Loss of nonsense mediated decay suppresses mutations in Saccharomyces cerevisiae TRA1
Suppression by upf1Δ is specific for mutations within the FATC domain of Tra1. A. upf1Δ suppression of tra1-F3744A. Serial dilutions of CY4353 (TRA1 UPF1), CY4350 (tra1-F3744A UPF1), CY5932 (TRA1 upf1Δ), and CY6030 (tra1-F3744A upf1Δ) were spotted onto YPD at 30°C or 37°C, or YPD containing 6% ethanol at 30°C. B. upf1Δ suppression of tra1-SRR3413. Serial dilutions of BY4742 (TRA1 UPF1), CY5932 (TRA1 upf1Δ), CY2200 (tra1-SRR3413 UPF1), and CY6111 (tra1-SRR3413 upf1Δ) were spotted onto YPD at 30°C or 37°C, or YPD containing 6% ethanol at 30°C. C. upf1Δ does not suppress ada2Δ. Serial dilutions of CY4353 (ADA2 UPF1), BY4282 (ada2Δ UPF1), CY5932 (ADA2 upf1Δ), and CY5979 (ada2Δ upf1Δ) haploids were spotted onto the indicated YPD plates. D. upf1Δ does not suppress eaf3Δ or eaf7Δ. Serial dilutions of CY4353 (WT), CY5932 (upf1Δ), BY7143 (eaf3Δ UPF1), CY5980 (eaf3Δ upf1Δ), BY2940 (eaf7Δ UPF1), and CY5976 (eaf7Δ upf1Δ) haploids were spotted onto YPD at 30°C or YPD containing 6% at 35°C.