Figure 1

Sensitivities of mcm2 alleles to genotoxic agents. The growth of phosphorylation site mutations in mcm2 was compared on plates containing genotoxic agents. The mec2-1 strain [24] was utilized to control for plate quality. A) The growth of mcm2_AA and MCM2 strains was compared on YPD and YPD containing 0.03% MMS, 400 μM 5-FU or 200 mM HU by spotting 10-fold serial dilutions of the strains followed by growth at 30°C for the indicated times. B) Similarly, the growth of mcm2_AA and MCM2 strains was compared on YPD with and without phleomycin. C) The growth of mcm2_EEmcm2_S164Emcm2_S170E and MCM2 on YPD with 0.03% MMS, 400 μM 5-FU or 200 mM HU.