Table 5 Primer sequences used for the generation of sugarcane retrotransposons-based markers
From: A novel linkage map of sugarcane with evidence for clustering of retrotransposon-based markers
Primer | Sequence 5′→3′ a |
|---|---|
Short arm oligonucleotide (AS) | TGGGATCTATACTT - H2N |
Long arm oligonucleotide (LA) | ACTCGATTCTCAACCCGAAAGTATAGATCCCA |
Adapter primer (AP) | ACTCGATTCTCAACCCGAAAG |
scIvana1_SSAP1 (sIvSI) | CAAGCCCTTAATAGCAGAAA |
scIvana1_GagRev (sIvGR) | TCCCTGTATACAACCCTGTC |
scIvana1_LTR1 (sIvLI) | AGTCCTGCTCCCAGTTATCA |
scIvana1_LTR2 (sIvLII) | GTCGCCTGGGTGTGTTATC |
scAle1_LTRr (sAlLr) | ATACATGGGCCACATGGG |
scAle1_RT (sAlRT) | CCTCCCDTCCTCGACCTTC |
scAle1_LTR1 (sAlLI) | CCATGWGRCTAGGCCCATGTGGC |
scAle1_LTR2 (sAlLI) | GGGGTGTTGGAGTGTGATTG |