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Table 2 Similarity of turbot EST-linked and anonymous sequences against model teleost genomes

From: An Expressed Sequence Tag (EST)-enriched genetic map of turbot (Scophthalmus maximus): a useful framework for comparative genomics across model and farmed teleosts

Model species Gac Tni Ola Tru Dre
Total hits E < 10−5 (%) 246 (54.4) 179 (39.4) 186 (41.0) 194 (42.7) 91 (20.5)
 Unique hitsa (%) 210 (48.0) 151 (33.3) 146 (32.9) 162 (35.7) 69 (15.5)
 Multiple hitsb (%) 36 (7.9) 28 (6.3) 38 (8.4) 32 (7.2) 22 (5.0)
EST-linked sequences (E)
 Unique hitsa (%) 121 (60.8) 98 (49.2) 88 (44.2) 93 (46.7) 50 (25.1)
 Unique_L–Lc (%) 98 (49.2) 60 (30.2) 70 (35.2) 66 (33.2) 44 (22.1)
 Multiple hitsb (%) 25 (12.6) 21 (10.6) 32 (16.1) 25 (12.6) 19 (9.6)
Anonymous sequences (A)
 Unique hitsa (%) 89 (36.3) 53 (26.6) 57 (22.4) 69 (27.1) 19 (7.8)
 Unique_L–Lc (%) 78 (31.8) 40 (16.3) 53 (21.6) 66 (26.9) 17 (6.9)
 Multiple hitsb (%) 11 (4.3) 7 (2.9) 7 (2.7) 7 (2.9) 3 (1.2)
Unique hits summary
 Mean size alignment bp 119.0 106.4 110.3 109.0 107.1
 Mean size A-Ed bp 100.0–133.0 91.8–115.0 94.4–119.4 94.7–119.8 84.0–114.0
 Maximum size A-Ed bp 257–504 252–401 215–422 222–438 181–295
 Mean E-value 1.4E-07 4.2E-07 3.6E-07 4.0E-07 6.1E-07
Minimun E-value 1.0E-140 1.0E-116 1.0E-120 1.0E-119 2.0E-62
 Identity % Mean 89.8 89.9 89.0 89.5 88.2
 Identity % Range 79.4–100 80.4–100 79.9–96.7 79.4–100 80.3–96.2
 Retained at E ≤ 10−10 (%) 182 (87%) 122 (81%) 110 (75%) 129 (80%) 50 (72%)
  1. BLASTn matches of 454 turbot microsatellite and SNP flanking sequences (199 EST-linked to 255 anonymous ones) against the stickleback (Gac: Gasterosteous aculeatus), spotted green puperfish (Tni: Tetraodon nigroviridis), medaka (Ola: Oryzias latypes), fugu (Tru: Takifugu rubripes) and zebrafish (Dre: Danio rerio) genomes at E < 10−5 threshold, most of which retained at E ≤ 10−10. aUnique or bMultiple hits: turbot sequences yielding a single significant match or ≥2 significant matches, respectively, against model species genomes. cUnique_L–L: linked markers at the turbot genetic map having unique significant matches with chromosome assignment on the model species genomes. dA-E: size alignment figures in base pairs (bp) for Anonymous (A) to EST-linked (E) markers.