A nonsense mutation in the tyrosinase gene causes albinism in water buffalo

BMC Genomic Data

Table 1 Primers used for RT-PCR amplification of B. bubalis tyrosinase mRNA

	Name	Primer Sequence (5´- 3´)	Product Size (bp)	Annealing Temperature
1	ASB.TYR - F1	TGAAAGGGAAGAGTGTGGCTCCAT	411	60 °C
	ASB.TYR - R8	TCGCCTTTCTGTGCAGCGGG	411	60 °C
2	JP.TYR - F2	AGTCTTGGCCCTCCATCTTT	350	60 °C
	JP.TYR - R2	GACTTCAGAGTCCCCAAGCA	350	60 °C
3	ASB.TYR - F6	TCCCCACGGGCACCTATGGC	419	60 °C
	ASB.TYR - R1	GTTGCATAAAGCCTGGCGACTGTT	419	60 °C
4	ASB.TYR - F7	CATGGGAGGGCGCAACCCTG	533	60 °C
	ASB.TYR - R2	AAGGAACCATGTAGGATTCCCGGT	533	60 °C
5	ASB.TYR - F2	AACAGTCGCCAGGCTTTATGCAAC	439	60 °C
	ASB.TYR - R2	AAGGAACCATGTAGGATTCCCGGT	439	60 °C
6	JP.TYR - F4	GATCTGCCAATGATCCCATC	329	60 °C
	JP.TYR - R4	AAGGACAGACCCAACCACAG	329	60 °C
7	ASB.TYR - F3	TAGAACAAGCACAACGAATCTGGC	377	60 °C
	ASB.TYR - R3	AAAGCAAGCACAGGTGGCTTCTAC	377	60 °C
8	ASB.TYR - F4	ACCGGGAATCCTACATGGTTCCTT	225	60 °C
	ASB.TYR - R4	TAAGTCCTCCCAGCACAGCAGTAA	225	60 °C
9	ASB.TYR - F5	AATGTAGCCCTCCTCCTACTCAGGTA	215	60 °C
	ASB.TYR - R5	GGGAACAAGTCATTCCACAATCAAGAGG	215	60 °C
10	JP.TYR - F6	CAATAGAGCTGGGGCAAAAA	247	60 °C
	JP.TYR - R6	TACCAAATGGCATCCTTTCC

ISSN: 2730-6844