PPP1R13L expression in the developing eyelid. Immunostaining for PPP1R13L (green) showed expression of PPP1R13L at E14.5-P0.5 (A, C, E, G) in the palpebral epidermis, palpebral conjunctiva, bulbar conjunctiva, corneal epithelium, lens epithelium and retina. DAPI (blue) was used as a nuclear stain. As eyelid closure begins, at E15.5 (C) PPP1R13L is also expressed in the leading edge of the migrating eyelid. As eyelid closure reaches completion at E16.5 (E), expression is maintained in the eyelid junction. At P0.5 (G), PPP1R13L is expressed in the developing meibomian gland (G arrow) and hair follicle (G arrowhead). Scale bars = 50 μm.