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Table 2 Precision and accuracy of the telomere and reference gene reactions quantified in terms of the standard deviation of Cq values (Cq SD), as well as the correlation coefficient (R 2 ) and the slope of the regression line between expected and observed amount log DNA as illustrated in Figure 1

From: Empirical evaluation of humpback whale telomere length estimates; quality control and factors causing variability in the singleplex and multiplex qPCR methods

   

Pfaffl

  

Ruijter

  

Target

Assay

Cq SD

R2

Slope

SD

R2

Slope

SD

Telomere

I

0.330

95.6

1.00

0.021

95.8

1.00

0.021

 

II

0.171

98.5

0.99

0.019

99.0

0.99

0.016

 

III

0.115

99.4

1.00

0.012

99.4

1.00

0.012

 

IV

0.116

99.2

1.00

0.012

99.5

1.01

0.010

Reference

I

0.423

95.7

1.06

0.027

95.6

1.00

0.021

 

II

0.476

92.0

0.98

0.045

93.4

1.02

0.044

 

III

0.264

97.0

0.99

0.027

93.4

0.97

0.039

 

IV

0.196

96.4

0.97

0.033

97.6

1.02

0.022