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Table 2 Precision and accuracy of the telomere and reference gene reactions quantified in terms of the standard deviation of Cq values (Cq SD), as well as the correlation coefficient (R 2 ) and the slope of the regression line between expected and observed amount log DNA as illustrated in Figure 1

From: Empirical evaluation of humpback whale telomere length estimates; quality control and factors causing variability in the singleplex and multiplex qPCR methods

    Pfaffl    Ruijter   
Target Assay Cq SD R2 Slope SD R2 Slope SD
Telomere I 0.330 95.6 1.00 0.021 95.8 1.00 0.021
  II 0.171 98.5 0.99 0.019 99.0 0.99 0.016
  III 0.115 99.4 1.00 0.012 99.4 1.00 0.012
  IV 0.116 99.2 1.00 0.012 99.5 1.01 0.010
Reference I 0.423 95.7 1.06 0.027 95.6 1.00 0.021
  II 0.476 92.0 0.98 0.045 93.4 1.02 0.044
  III 0.264 97.0 0.99 0.027 93.4 0.97 0.039
  IV 0.196 96.4 0.97 0.033 97.6 1.02 0.022