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Table 4 Basic characteristics of telomere lengths in humpback whales determined by different assays and quantitative methods

From: Empirical evaluation of humpback whale telomere length estimates; quality control and factors causing variability in the singleplex and multiplex qPCR methods

   

T/S

   

SD

  

CV%

   

Assay

Method

N

Av

Min

Max

dMinMax

Av

Min

Max

Av

Min

Max

SD/(dMinMax)%

I

Pfaffl LR

58

1.05

0.38

1.89

1.52

0.16

0.01

0.71

19.0

1.8

52.0

10.9

 

Pfaffl RG

60

1.29

0.38

2.42

2.04

0.22

0.01

0.47

15.8

1.6

47.8

11.0

 

Ruijter

60

1.36

0.36

2.60

2.24

0.25

0.02

0.81

17.1

4.7

41.2

11.2

 

ddCq

58

1.19

0.29

2.17

1.88

0.23

0.01

0.80

17.9

1.6

49.5

12.4

II

Pfaffl LR

54

0.84

0.15

1.73

1.57

0.19

0.01

0.71

21.4

3.3

72.1

12.1

 

Pfaffl RG

49

0.77

0.15

1.80

1.65

0.31

0.03

1.86

35.5

6.0

131.0

19.1

 

Ruijter

51

0.73

0.15

1.51

1.36

0.16

0.01

0.58

21.8

3.3

72.1

11.9

 

ddCq

54

3.38

0.07

20.46

20.39

1.21

0.02

9.76

30.3

3.6

93.2

5.9

III

Pfaffl LR

54

0.80

0.24

1.39

1.15

0.20

0.00

1.22

25.1

0.2

89.1

17.1

 

Pfaffl RG

55

0.87

0.19

1.62

1.43

0.31

0.04

1.12

49.6

4.5

372.4

21.6

 

Ruijter

56

0.82

0.17

1.40

1.23

0.21

0.01

1.25

26.4

0.6

89.1

17.3

 

ddCq

54

0.73

0.19

1.94

1.75

0.22

0.00

1.39

30.6

0.1

111.9

12.3

IV

Pfaffl LR

59

1.58

0.44

2.87

2.43

0.14

0.01

0.42

8.7

1.3

29.6

5.7

 

Pfaffl RG

59

1.61

0.45

3.15

2.70

0.14

0.02

0.51

9.0

0.6

30.8

5.3

 

Ruijter

59

1.54

0.45

2.62

2.16

0.13

0.01

0.44

8.8

1.3

29.6

6.2

 

ddCq

60

1.53

0.45

2.93

2.48

0.12

0.01

0.31

8.2

1.3

27.2

4.9

  1. N = number of samples after outlier removal; T/S = Telomere length; Av = average; Min = minimum T/S; Max = maximum T/S; dMinMax = difference between minimum and maximum T/S; SD = standard deviation of T/S estimates; CV% = the coefficient of variation of T/S estimates in percent; SD/(dMinMax) = the ratio in percent between SD and the difference between minimum and maximum T/S. Pfaffl LR = the Pfaffl method with baseline corrected in LinRegPCR; Ruijter = the Ruijter method; ddCq = the comparative Cq method; Pfaffl RG = the Pfaffl method with baseline corrected in Rotor-Gene or ABI software. See text for details.
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BMC Genomic Data

ISSN: 2730-6844

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