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Table 4 Basic characteristics of telomere lengths in humpback whales determined by different assays and quantitative methods

From: Empirical evaluation of humpback whale telomere length estimates; quality control and factors causing variability in the singleplex and multiplex qPCR methods

    T/S     SD    CV%    
Assay Method N Av Min Max dMinMax Av Min Max Av Min Max SD/(dMinMax)%
I Pfaffl LR 58 1.05 0.38 1.89 1.52 0.16 0.01 0.71 19.0 1.8 52.0 10.9
  Pfaffl RG 60 1.29 0.38 2.42 2.04 0.22 0.01 0.47 15.8 1.6 47.8 11.0
  Ruijter 60 1.36 0.36 2.60 2.24 0.25 0.02 0.81 17.1 4.7 41.2 11.2
  ddCq 58 1.19 0.29 2.17 1.88 0.23 0.01 0.80 17.9 1.6 49.5 12.4
II Pfaffl LR 54 0.84 0.15 1.73 1.57 0.19 0.01 0.71 21.4 3.3 72.1 12.1
  Pfaffl RG 49 0.77 0.15 1.80 1.65 0.31 0.03 1.86 35.5 6.0 131.0 19.1
  Ruijter 51 0.73 0.15 1.51 1.36 0.16 0.01 0.58 21.8 3.3 72.1 11.9
  ddCq 54 3.38 0.07 20.46 20.39 1.21 0.02 9.76 30.3 3.6 93.2 5.9
III Pfaffl LR 54 0.80 0.24 1.39 1.15 0.20 0.00 1.22 25.1 0.2 89.1 17.1
  Pfaffl RG 55 0.87 0.19 1.62 1.43 0.31 0.04 1.12 49.6 4.5 372.4 21.6
  Ruijter 56 0.82 0.17 1.40 1.23 0.21 0.01 1.25 26.4 0.6 89.1 17.3
  ddCq 54 0.73 0.19 1.94 1.75 0.22 0.00 1.39 30.6 0.1 111.9 12.3
IV Pfaffl LR 59 1.58 0.44 2.87 2.43 0.14 0.01 0.42 8.7 1.3 29.6 5.7
  Pfaffl RG 59 1.61 0.45 3.15 2.70 0.14 0.02 0.51 9.0 0.6 30.8 5.3
  Ruijter 59 1.54 0.45 2.62 2.16 0.13 0.01 0.44 8.8 1.3 29.6 6.2
  ddCq 60 1.53 0.45 2.93 2.48 0.12 0.01 0.31 8.2 1.3 27.2 4.9
  1. N = number of samples after outlier removal; T/S = Telomere length; Av = average; Min = minimum T/S; Max = maximum T/S; dMinMax = difference between minimum and maximum T/S; SD = standard deviation of T/S estimates; CV% = the coefficient of variation of T/S estimates in percent; SD/(dMinMax) = the ratio in percent between SD and the difference between minimum and maximum T/S. Pfaffl LR = the Pfaffl method with baseline corrected in LinRegPCR; Ruijter = the Ruijter method; ddCq = the comparative Cq method; Pfaffl RG = the Pfaffl method with baseline corrected in Rotor-Gene or ABI software. See text for details.