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Table 5 Summary of quality control steps and overall assay performance for the telomere (T), reference (S), and telomere length estimates (T/S)

From: Empirical evaluation of humpback whale telomere length estimates; quality control and factors causing variability in the singleplex and multiplex qPCR methods

Criteria Target Assay I Assay II Assay III Assay IV Figure/Table
Serial dilution series       
LDR T Borderline Within range Within range Within range Figure 2
  S Borderline Within range Within range Within range  
NTC T OK Borderline Borderline OK Figures 3, 4
  S OK Overlap* Overlap Overlap*  
Magnitude E T Intermediate Intermediate Intermediate High Figure 1
  S Intermediate Low Intermediate Intermediate  
Interbatch E T Constant Variable Constant Constant Figure 1
  S Constant Variable Constant Constant  
Dilution E T Variable E No change No change No change Table 3
  S Variable E No change No change No change  
Precision T Intermediate High High High Figure 5, Table 4
  S Intermediate Low Moderate High  
Accuracy T High High High High Table 4
  S High Intermediate High Intermediate  
Resolution T Low High High High Table 5
  S Intermediate Intermediate High High  
Precision T/S Intermediate Low Intermediate Intermediate Additional file 3: Figure S2
Accuracy T/S Low Low High High Additional file 3: Figure S2
"Unknown" samples       
Outliers T/S Few Many Many Few Additional file 1: Table S1
Resolution T/S Intermediate Low Low High Additional file 1: Table S1
Quantitative method T/S High Low Intermediate High Additional file 5: Table S2
Inter-assay correlation T/S Low Low Low Intermediate Additional file 4: Figure S3
Assay evaluation   Intermediate Worst Intermediate Best