Genetic interactions between mRNA export factors and components of the transcription machinery. (A) Suppression of rat8-2 by depletion of the TFIIH component Tfb1p. rat8-2 single mutant and rat8-2 P.
-TFB1 double mutant were spotted onto YPD or YPD containing 10 mg/L of doxycycline plates and incubated at the indicated temperatures for 4 days. (B) Synthetic sickness of rat8-2 and toa2 mutations. Serial dilutions of single and double mutants strains were spotted onto YPD plates and incubated at different temperatures for 4 days. (C) Mutation in toa1 impairs growth of rat8-2 mutants. Strains analyzed were segregants from the cross CSY550 (rat8-2) x MY1870 (toa1-2). Cells were spotted onto a YPD plate and incubated for 3 days at the indicated temperatures. (D) Synthetic lethality between rat8-2 and sua7-4. Double mutants rat8-2 sua7:: LEU2 [pSUA7/URA3] carrying the wild type SUA7 gene or the mutant allele sua7-4 in a HIS3/CEN plasmid were spotted on YPD or 5′FOA plates and incubated at 30 °C for 4 days