Table 1 Comparison of the key steps of gDNA extraction protocols from the three different starting materials
DNA isolation step | Starting material | ||
|---|---|---|---|
3.2 mm DBS | 100 μl WB | 0.5 g UC | |
1. DNA binding column | Silica-based | Silica-based | Silica-based |
2. Use of carrier RNA* | Yes | Yes | No |
3. Duration of lysis step | 1 h | 1 h | ~12 h |
4. Elution volume | 60 μl | 100 μl | 1 ml |
5. Solution used for elution | Nuclease free water | Nuclease free water | Nuclease free water |